High growth methanotrophic bacterial strain

ABSTRACT

A high growth methanotrophic bacterial strain capable of growth on a C1 carbon substrate has been isolated and characterized. The strain has the unique ability to utilize both methane and methanol as a sole carbon source and has been demonstrated to possess a functional Embden-Meyerhof carbon flux pathway. The possession of this pathway conveys an energetic advantage to the strain, making it particularly suitable as a production platform for the production of biomass from a C1 carbon source.

[0001] This application claims the benefit of U.S. ProvisionalApplication No. 60/229,858 filed Sep. 1, 2000.

FIELD OF THE INVENTION

[0002] The invention relates to the field of microbiology. Morespecifically, the invention relates to the use of a novel methanotrophicbacterial strain capable of utilizing a central carbon pathway for moreefficient production of commercially useful products.

BACKGROUND OF THE INVENTION

[0003] Methanotrophic bacteria are defined by their ability to usemethane as their sole source of carbon and energy. Although methanol isan obligate intermediate in the oxidation of methane, the ability togrow on methanol alone is highly variable among the obligatemethanotrophs due to its toxicity (Green, Peter. Taxonomy ofMethylotrophic Bacteria. In: Methane and Methanol Utilizers(Biotechnology Handbooks 5) J. Colin Murrell and Howard Dalton eds. 1992Pleanum Press NY, pp. 23-84). Methane monooxygenase is the enzymerequired for the primary step in methane activation and the product ofthis reaction is methanol (Murrell et al., Arch. Microbiol. (2000),173(5-6), 325-332). This reaction occurs at ambient temperatures andpressures, whereas chemical transformation of methane to methanolrequires temperatures of hundreds of degrees and high pressure(Grigoryan, E. A., Kinet. Catal. (1999), 40(3), 350-363; WO 2000007718;U.S. Pat. No. 5,750,821). It is this ability to transform methane underambient conditions along with the abundance of methane that makes thebiotransformation of methane a potentially unique and valuable process.

[0004] The commercial applications of biotransformation of methane havehistorically fallen broadly into three categories: 1) Production ofsingle cell protein, (Sharpe D. H. BioProtein Manufacture (1989). EllisHorwood series in applied science and industrial technology. New York:Halstead Press) (Villadsen, John, Recent Trends Chem. React. Eng.,[Proc. Int. Chem. React. Eng. Conf.], 2nd (1987), Volume 2, 320-33.Editor(s): Kulkarni, B. D.; Mashelkar, R. A.; Sharma, M. M. Publisher:Wiley East., New Delhi, India; Naguib, M., Proc. OAPEC Symp.Petroprotein, [Pap.] (1980), Meeting Date 1979, 253-77 Publisher: Organ.Arab Pet. Exporting Countries, Kuwait, Kuwait); 2) epoxidation ofalkenes for production of chemicals (U.S. Pat. No. 4,348,476); and 3)biodegradation of chlorinated pollutants (Tsien et al., Gas, Oil, Coal,Environ. Biotechnol. 2, [Pap. Int. IGT Symp. Gas, Oil, Coal, Environ.Biotechnol.], 2nd (1990), 83-104. Editor(s): Akin, Cavit; Smith, Jared.Publisher: Inst. Gas Technol., Chicago, Ill.; WO 9,633,821; Merkley etal., Biorem. Recalcitrant Org., [Pap. Int. In Situ On-Site Bioreclam.Symp.], 3rd (1995), 165-74. Editor(s): Hinchee, Robert E; Anderson,Daniel B.; Hoeppel, Ronald E. Publisher: Battelle Press, Columbus,Ohio.: Meyer et al., Microb. Releases (1993), 2(1), 11-22). Onlyepoxidation of alkenes has experienced little commercial success due tolow product yields, toxicity of products and the large amount of cellmass required to generate products.

[0005] Large-scale protein production from methane, termed single cellprotein or SCP has been technically feasible and commercialized at largescale (Villadsen supra). However, SCP has been less than economicallysuccessful due to the relatively high cost to produce microbial proteincompared to agriculturally derived protein (i.e. soy protein). Singlecell protein is a relatively low value product and therefore economicproduction cannot tolerate heavy bioprocessing costs. For this reasonthe yield of the methanotrophic strain may be critical to the overalleconomic viability of the process. Microbial biomass produced bymethanotrophic bacteria is typically very high in protein content(˜70-80% by weight), which can restrict the direct use of this proteinto certain types of animal feed.

[0006] The conversion of C1 compounds to complex molecules with C—Cbonds is a difficult and capital intensive process by traditionalchemical synthetic routes. Traditionally, methane is first converted tosynthesis gas (mixtures of hydrogen, carbon monoxide and carbondioxide), which is then used to produce other small molecular weightindustrial precursors. Typically these are “commodity” type chemicalssuch as acetate, formaldehyde, or methanol. The basic problem isactivation of the methane molecule which is thermodynamically verydifficult to achieve by chemical means. “Activation” refers to theprocess of making the chemically unreactive methane molecule morereactive.

[0007] Methanotrophic bacteria contain enzymes (methane monooxygenases)which are capable of methane activation at ambient temperatures andpressures. Methane activation consists of oxygen insertion into methaneto form methanol which is much more readily metabolized to more complexmolecules within the cell. Two types of methane monooxygenase are foundin methanotrophic bacteria. A particulate methane monooxygenase (pMMO)has a narrow substrate specificity and is incapable of oxygen insertioninto more complex molecules. Some, but not all methanotrophs may alsocontain a soluble methane monooxygenase (sMMO). This enzyme has been thesubject of much investigation and proprietary claims due to its abilityto oxygenate, or functionalize, a wide variety of aliphatic and aromaticmolecules. This characteristic has been utilized for co-metabolicproduction processes where methanotrophs are fed both methane and a morecomplex molecule to be transformed by the sMMO. Numerous examples arereported of processes requiring both methane and, typically, apetroleum-derived feedstock such as toluene, naphthalene, or decane,where sMMO plays a role. However, the art is silent with respect tousing methanotrophs for net synthesis of chemicals from methane asopposed to these co-metabolic transformations. For net synthesis, onlyinexpensive methane is required along with the ability to geneticallyengineer the strain to produce the desired chemical.

[0008] Methanotrophic cells can further build the oxidation products ofmethane (i.e. methanol and formaldehyde) into more complex moleculessuch as protein, carbohydrate and lipids. For example, under certainconditions methanotrophs are known to produce exopolysaccharides(Ivanova et al., Mikrobiologiya (1988), 57(4), 600-5; Kilbane, John J.,II Gas, Oil, Coal, Environ. Biotechnol. 3, [Pap. IGT's Int. Symp.], 3rd(1991), Meeting Date 1990, 207-26. Editor(s): Akin, Cavit; Smith, Jared.Publisher: IGT, Chicago, Ill.). Similarly, methanotrophs are known toaccumulate both isoprenoid compounds and carotenoid pigments of variouscarbon lengths (Urakami et al., J. Gen. Appl. Microbiol. (1986), 32(4),317-41). Although these compounds have been identified in methanotrophs,they have not been microbial platforms of choice for production as theseorganisms have very poorly developed genetic systems, thereby limitingmetabolic engineering for chemicals.

[0009] A necessary prerequisite to metabolic engineering ofmethanotrophs is a full understanding, and optimization, of the carbonmetabolism for maximum growth and/or product yield. Obligatemethanotrophs are typically thought to channel carbon from methane touseful products and energy via the Entner-Douderoff Pathway whichutilizes the keto-deoxy phosphogluconate aldolase enzyme (Dijkhuizen,L., P. R. Levering, G. E. DeVries 1992. In: Methane and MethanolUtilizers (Biotechnology Handbooks 5) J. Colin Murrell and Howard Daltoneds. 1992 Pleanum Press NY pp 149-181). This pathway is notenergy-yielding as is the case for the Embden-Meyerhof pathway. Thus,utilization of the Entner-Douderoff pathway results in lower cellularproduction yields and a greater proportion of the carbon produced ascarbon dioxide compared to organisms that use the Embden-Meyerhofpathway. Therefore, a more energy efficient carbon processing pathwaywould greatly enhance the commercial viability of a methanotrophicplatform for the generation of materials.

[0010] As noted above, methanotrophic bacteria possess the potential tobe commercially effective production platforms for materials such assingle cell protein, exopolysaccharides, and long chain carbon moleculessuch as isoprenoids and carotenoid pigments. The usefulness ofmethanotrophs for production of a larger range of chemicals isconstrained however, by several limitations including, relatively slowgrowth rates of methanotrophs, limited ability to tolerate methanol asan alternative substrate to methane, difficulty in genetic engineering,poor understanding of the roles of multiple carbon assimilation pathwayspresent in methanotrophs, and potentially high costs due to the oxygendemand of fully saturated substrates such as methane. The problem to besolved therefore is to develop a fast-growing, high yieldingmethanotroph capable of receiving foreign genes via standard geneticprocedures. Full and rapid resolution of central carbon pathways isessential for enabling pathway engineering and carbon flux managementfor new products.

[0011] Applicants have solved the stated problem by providing amethanotrophic bacterial strain capable of efficiently using eithermethanol or methane as a carbon substrate. The strain is alsometabolically versatile in that it contains multiple pathways for theincorporation of carbon from formaldehyde into 3-C units. The discoveryof a phosphofructokinase and fructose 1,6 bisphosphate aldolase in thisstrain suggests that it can utilize the more energetically favorableEmbden-Meyerhof pathway in addition to the Entner-Douderoff pathways.The present strain is shown to be useful for the production of a varietyof materials beyond single cell protein to include carbohydrates,pigments, terpenoid compounds and aromatic compounds. The formation oflarge amounts of carbohydrates from methane or methanol can be carriedout by this strain. This is surprising and also enables this strain tobe used for the production of typical carbohydrate or sugar fermentationend-products such as alcohols, acids and ketones. The present strain wasalso shown to be capable of genetic exchange with donor species such asEscherichia coli via a standard genetic procedure known as bacterialconjugation. In this way, the strain can be engineered for net synthesisfrom methane to produce new classes of products other than thosenaturally produced.

SUMMARY OF THE INVENTION

[0012] The present invention provides a methanotrophic bacterial straincapable of growth on a C1 carbon substrate. The instant bacterial strainmay be further characterized by the ability to grow rapidly andefficiently on either methanol or methane as a sole carbon source. Thisefficiency is due to the presence of a pyrophosphate linkedphosphofructokinase enzyme within an operative Embden-Meyerhof pathway.This is a novel observation for methanotrophic bacteria. Functionally,the utilization of the Emben-Meyerhof pathway and pyrophosphate, insteadof the Entner-Douderoff pathway reaction results in highly favorablecellular energetics which is manifested in higher yields, carbonconversion efficiency and growth rate.

[0013] The present strain also contains an enzyme system capable ofreducing nitrate or nitrite with formation of gaseous nitrogen oxides.This capability is useful for reducing oxygen demand as well as forremoving nitrates and nitrites in methane-containing environments suchas landfills, wastewater treatment systems or anywhere that methane,oxygen and nitrates are present.

[0014] The ability to form large amounts of carbohydrates in the form ofstarch, polyglucose and/or extracellular polysaccharide is also usefulfor the production of carbohydrate-based products. AdditionallyMethylomonas 16a is only capable of growth on methane or methanol and isincapable of proliferating in the human body and thus is completelyharmless and non-pathogenic. These characteristics make the strainideally useful for the production of a wide range of products includinganimal feeds comprising variable carbohydrate/protein ratios.

[0015] The strain is shown to be capable of genetic exchange andexpression of foreign genes. Additionally the present strain may beidentified by the characteristic 16sRNA sequence as set forth in SEQ IDNO:81.

[0016] Additionally the present invention provides methods for theproduction of single cell protein, carbohydrates, and carotenoidpigments, or higher value mixtures of protein, pigments andcarbohydrates. Additionally the strain may be used as a denitrifyingagent for the conversion of nitrate or nitrite to nitrous oxide withmethane or methanol as carbon source.

[0017] Accordingly the invention provides a high growth methanotrophicbacterial strain which:

[0018] (a) grows on a C1 carbon substrate selected from the groupconsisting of methane and methanol; and

[0019] (b) comprises a functional Embden-Meyerhof carbon pathway, saidpathway comprising a gene encoding a pyrophosphate dependentphosphofructokinase enzyme, the gene selected from the group consistingof:

[0020] (a) an isolated nucleic acid molecule encoding the amino acidsequence as set forth in SEQ ID NO:6;

[0021] (b) an isolated nucleic acid molecule that hybridizes with (a)under the following hybridization conditions: 0.1×SSC, 0.1% SDS, 65° C.and washed with 2×SSC, 0.1% SDS followed by 0.1×SSC, 0.1% SDS;

[0022] (c) an isolated nucleic acid molecule comprising a firstnucleotide sequence encoding a polypeptide of at least 437 amino acidsthat has at least 63% identity based on the Smith-Waterman method ofalignment when compared to a polypeptide having the sequence as setforth in SEQ ID NO:6; and

[0023] (d) an isolated nucleic acid molecule that is complementary to(a), (b) or (c).

[0024] Optionally the present strain may comprise at least one geneencoding a fructose bisphosphate aldolase enzyme as part of thefunctional Embden-Meyerhof carbon pathway. Additionally, the presentstrain may optionally contain a functional Entner-Douderoff carbonpathway, where the Entner-Douderoff carbon pathway comprises at leastone gene encoding a keto-deoxy phosphogluconate aldolase.

[0025] In one embodiment the present strain may optionally contain othercarbon flux genes encoding polypeptides selected from the groupconsisting of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18 and 20.

[0026] In another embodiment the present strain may possess adenitrification pathway where the pathway may optionally comprise genesencoding polypeptides having the amino acid sequences selected from thegroup consisting of SEQ ID NO:40, 42, 44, 46, 48, 50, 52, 54, 56, 58 and60.

[0027] In another embodiment the present strain may contain a set ofexopolysaccharide synthesizing enzymes where the exopolysaccharidesynthesizing enzymes may have the amino acid sequences selected from thegroup consisting of SEQ ID NO:22, 24, 26, 28, 30, 32, 34, 36, and 38.

[0028] In a more specific embodiment the present strain may comprisegenes encoding isoprenoid synthesizing enzymes where the enzymes areselected from the group consisting of SEQ ID NO:62, 64, 66, 68, 70, 72,74, 86, and 78.

[0029] In a preferred embodiment the invention provides a method for theproduction of single cell protein comprising:

[0030] a) contacting the present high growth methanotrophic bacterialstrain with a C1 carbon substrate, selected from the group consisting ofmethane and methanol, in a suitable medium for a time sufficient topermit the expression and accumulation of single cell protein; and

[0031] b) optionally recovering the single cell protein.

[0032] It is an additional object of the invention to provide a methodfor the biotransformation of a nitrogen containing compound selectedfrom the group consisting of ammonia, nitrate, nitrite, and dinitrogencomprising, contacting the present high growth methanotrophic bacterialstrain with a C1 carbon substrate selected from the group consisting ofmethane or methanol, in the presence of the nitrogen containingcompound, in a suitable medium for a time sufficient to permit thebiotransformation of the nitrogen containing compound.

[0033] Similarly it is an object of the present invention to provide amethod for the production of a feed product comprising protein,carbohydrates and pigment comprising the steps of:

[0034] a) contacting the high growth methanotrophic bacterial strain ofthe present invention with a C1 carbon substrate in a suitable mediumfor a time sufficient to permit the expression and accumulation of thefeed product; and

[0035] b) optionally recovering the feed product.

[0036] Optionally the relative compositions of protein, carbohydrate andpigment are altered through the up-regulation or down-regulation of anyone of the genes encoding the proteins selected from the groupconsisting of SEQ ID NO:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25,27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61,63, 65, 67, and 69.

[0037] In a preferred embodiment the invention provides a method ofidentifying a high growth methanotrophic bacterial strain comprising:

[0038] (a) growing a sample suspected of containing a high growthmethanotrophic bacterial strain on a suitable growth medium in thepresence of methane as a sole carbon source;

[0039] (b) identifying colonies that grow under the conditions of step(a);

[0040] (c) analyzing the colonies identified in step (b) for thepresence of pyrophosphate dependent phosphofructokinase activity.

BRIEF DESCRIPTION OF THE DRAWINGS SEQUENCE DESCRIPTIONS AND BIOLOGICALDEPOSITS

[0041]FIG. 1 shows the growth of Methylomonas 16a compared to the growthof Methylococcus capsulatus under identical growth conditions.

[0042]FIG. 2 is a plot of optical density vs. methanol concentration fora culture of Methylomonas 16a grown on methanol alone.

[0043]FIG. 3 represents a schematic of the Entner-Douderoff andEmbden-Meyerhof pathways in Methylomonas 16a showing microarrayexpression results numerically ranked in order of decreasing expressionlevel.

[0044]FIG. 4 shows oxygen uptake by a cell suspension of Methylomonas16a, in arbitrary units to detect oxygen consumption.

[0045]FIG. 5 shows oxygen uptake by a cell suspension of Methylomonas16a, in arbitrary units to detect oxygen consumption before and aftersodium nitrite was injected into the incubation.

[0046]FIG. 6 is a plot of the concentration of O₂ and N₂O evolved perhour vs. the concentration of O₂ in the medium of a cell suspension ofMethylomonas 16a under aerobic conditions.

[0047] The invention can be more fully understood from the followingdetailed description and the accompanying sequence descriptions whichform a part of this application.

[0048] The following sequence descriptions and sequences listingsattached hereto comply with the rules governing nucleotide and/or aminoacid sequence disclosures in patent applications as set forth in 37C.F.R. §1.821-1.825. The Sequence Descriptions contain the one lettercode for nucleotide sequence characters and the three letter codes foramino acids as defined in conformity with the IUPAC-IYUB standardsdescribed in Nucleic Acids Research 13:3021-3030 (1985) and in theBiochemical Journal 219 (No. 2):345-373 (1984) which are hereinincorporated by reference. The symbols and format used for nucleotideand amino acid sequence data comply with the rules set forth in 37C.F.R. §1.822. SEQ ID SEQ ID Description Nucleic acid PeptidePhosphoglucomutase: carbon Flux 1 2 Glucose 6 phosphate 3 4 isomerase:Carbon flux Phosphofructokinase pyrophosphate 5 6 dependent: Carbon Flux6-Phosphogluconate 7 8 dehydratase: Carbon flux Glucose 6 phosphate 1 910 dehydrogenase: Carbon Flux Transaldolase: Carbon Flux 11 12Transaldolase: Carbon Flux 13 14 Fructose bisphosphate 15 16 aldoslase:Carbon Flux Fructose bisphosphate 17 18 aldoslase: Carbon Flux KHG/KDPGAldolase: Carbon Flux 19 20 ugp: Exopolysaccharaide 21 22 gumD:Exopolysaccharaide 23 24 wza: Exopolysaccharaide 25 26 epsB:Exopolysaccharaide 27 28 epsM: Exopolysaccharaide 30 20 waaE:Exopolysaccharaide 31 32 epsV: Exopolysaccharaide 33 34 gumH:Exopolysaccharaide 35 36 glycosyl 37 38 transferase: ExopolysaccharaidenirF: Denitrification 39 40 nirD: Denitrification 41 42 nirL:Denitrification 43 44 nirG: Denitrification 45 46 nirH: Denitrification47 48 nirJ: Denitrification 49 50 nasA: Denitrification 51 52 norC:Denitrification 53 54 norB: Denitrification 55 56 norZ: Denitrification57 58 norS: Denitrification 59 60 dxs: Terpenoid synthesis 61 62 dxr:Terpenoid synthesis 63 64 ispF: Terpenoid synthesis 65 66 ispD:Terpenoid synthesis 67 68 pyrG: Terpenoid synthesis 69 70 IspA:Terpenoid synthesis 71 72 IspE: Terpenoid synthesis 73 74 crtN:Terpenoid synthesis 75 76 crtN1: Terpenoid synthesis 77 78 Particulatemonooxygenase 79 80 16sRNA for Methylomonas 16a 81 —

[0049] Applicants made the following biological deposits under the termsof the Budapest Treaty on the International Recognition of the Depositof Micro-organisms for the Purposes of Patent Procedure: InternationalDepositor Identification Depository Reference Designation Date ofDeposit Methylomonas 16a ATCC PTA 2402 Aug. 21, 2000

DETAILED DESCRIPTION OF THE INVENTION

[0050] The present invention describes the isolation andcharacterization of a high growth methanotrophic bacterial strain usefulfor the production of biomass including proteins, carbohydrates andpigments. The present strain is typed by 16sRNA as a Methylomonas sp.and is referred to herein as Methylomonas 16a. In addition, the strainmay be useful for the production of mixtures of proteins, carbohydratesand pigments for the purpose of generating animal feeds. The strainpossesses the advantage of an active Embden-Meyerhof carbon flux pathwayhaving a pyrophosphate dependent phosphofructokinase gene, which conveyscertain energetic advantages to the strain as a production platform forvarious materials and biomass. Additionally the strain naturallypossesses an active isoprenoid pathway for the generation of pigmentsindigenous to the strain. In this disclosure, a number of terms andabbreviations are used. The following definitions are provided.

[0051] The term “Embden-Meyerhof pathway” refers to the series ofbiochemical reactions for conversion of hexoses such as glucose andfructose to important cellular 3 carbon intermediates such asglyceraldehyde-3-phosphate, dihydroxyacetone phosphate, phosphophenolpyruvate and pyruvate. These reactions typically proceed with net yieldof biochemically useful energy in the form of ATP. The key enzymesunique to the Embden-Meyerhof pathway are phosphofructokinase andfructose-1,6 bisphosphate aldolase.

[0052] The term “Entner-Douderoff pathway” refers to a series ofbiochemical reactions for conversion of hexoses such as glucose orfructose to important 3 carbon cellular intermediates such as pyruvateand glyceraldehyde-3-phosphate without any net production ofbiochemically useful energy. The key enzymes unique to theEntner-Douderoff pathway are the 6 phosphogluconate dehydratase and theketodeoxyphosphogluconate aldolase.

[0053] The term “diagnostic” as it relates to the presence of a gene ina pathway means where a gene having that activity is identified, it isevidence of the presence of that pathway. Within the context of thepresent invention the presence of a gene encoding a pyrophosphatedependant phosphofructokinase is “diagnostic” for the presence of theEmbden-Meyerhof carbon pathway and the presence of gene encoding aketodeoxyphosphogluconate aldolase is “diagnostic” for the presence ofthe Entner-Douderoff carbon pathway.

[0054] The term “Yield” is defined herein as the amount of cell massproduced per gram of carbon substrate metabolized.

[0055] The term “carbon conversion efficiency” is a measure of how muchcarbon is assimilated into cell mass and is calculated assuming abiomass composition of CH₂O_(0.5)N_(0.25.)

[0056] The term “high growth methanotrophic bacterial strain” refers toa bacterium capable of growth with methane or methanol as a sole carbonand energy source and which possesses a functional Embden-Meyerhofcarbon flux pathway resulting in a yield of cell mass per gram of C1substrate metabolized. The specific “high growth methanotrophicbacterial strain” described herein is referred to as “Methylomonas 16a”or “16a”, which terms are used interchangeably.

[0057] The term “a C1 carbon substrate” refers to any carbon-containingmolecule that lacks a carbon-carbon bond. Examples are methane,methanol, formaldehyde, formic acid, methylated amines, and methylatedthiols.

[0058] The term “functional denitrifying enzymatic pathway” refers to aseries of enzymes which sequentially reduce nitrate or nitrite to morereduced products such as nitric oxide, nitrous oxide or ultimatelydinitrogen. This process may or may not be energy yielding.

[0059] The term “denitrification” refers to the process of convertingnitrates or nitrites to gaseous dinitrogen or other gaseous nitrogenoxides. To facilitate denitrification the present strain comprises genesencoding a number of enzymes in the denitrification pathway including:the nir genes (nirD, nirF, nirG, nirH, nirJ, nirL and nirS) encoding thenitrite reductase which catalyzes the reduction of nitrite (NO₂) tonitric oxide, the nasA gene, encoding nitrate reductase which catalyzesthe reduction of nitrate (NO₃) to nitrite (NO₂); and the nor genes(norB, norC or norZ) encoding a nitric oxide reductase which catalyzesthe reduction of nitric oxide (NO) to nitrous oxide (N₂O).

[0060] The term “isoprenoid compound” refers to any compound which isderived via the pathway beginning with isopentenyl pyrophosphate andformed by the head to tail condensation of isoprene units which may beof 5, 10, 15, 20, 30 or 40 carbons in length. The term “isoprenoidpigment” refers to a class of compounds which typically have stronglight absorbing properties and are derived from the head to tailcondensation of 5, 10, 15, 20, 25, 30 or 40 carbon isoprene chains.These isoprene chains are ultimately derived from isopentenylpyrophosphate. A number of genes and gene products are associated withthe present strain encoding the isoprenoid biosynthetic pathwayincluding the dxs gene, encoding 1-deoxyxylulose-5-phosphate synthase,the dxr gene, encoding 1-deoxyxylulose-5-phosphate reductoisomerase, the“ispD,” gene encoding the 2C-methyl-D-erythritol cytidyltransferaseenzyme, the “ispE”gene encoding the4-diphosphocytidyl-2-C-methylerythritol kinase, the “ispF” gene encodinga 2C-methyl-d-erythritol 2,4-cyclodiphosphate synthase, the “pyrG” gene,encoding a CTP synthase, the “ispA” gene, encoding geranyltransferase orfarneseyl diphosphate synthase and the “ctrN” and “ctrN 1” genes,encoding diapophytoene dehydrogenase.

[0061] The term “single cell protein” will be abbreviated “SCP” andrefers to a protein derived from organisms that exist in theunicellular, or single cell, state. This includes unicellular bacteria,yeasts, fungi or eukaryotic single cell organisms such as algae.

[0062] The term “extracellular polysaccharide” or “exocellularpolysaccharide” will be abbreviated “ESP” and refers to a polysaccharideproduced by methanotrophic bacteria typically comprising a carbohydrate“backbone” polymer as cross-linking carbohydrate polymers. Thesepolymers are excreted on the outside of the microbial cell and mayfunction in adhesion to surfaces or as a response to environmentalstress. The present strain comprises a number of genes encoding varioussteps in the synthesis of extracellular polysaccharide including the“ugp” gene encoding UDP-glucose pyrophosphorylase, the “gumD” and “WaaE”genes encoding glycosyltransferases, the “wza” and “epsB” genes,encoding polysaccharide export proteins, the “epsM” gene, encoding apolysaccharide biosynthesis related protein, and the “epsV”gene,encoding a sugar transferase.

[0063] The term “carbohydrate” refers to any sugar containingconstituent, particularly storage forms, such as glycogen or starch andextracellular polysaccharides.

[0064] The term “fermentation product” refers to products derived fromthe fermentation of any carbohydrate formed by the methanotrophicbacterium from methane or methanol.

[0065] The term “particulate methane monooxygenase” will be abbreviatedas “pMMO” and will refer to a membrane-associated methane monooxygenasewhich inserts oxygen in to the enzyme substrate.

[0066] The terms “soluble methane monooxygenase” will be abbreviated as“sMMO” and will refer a to soluble or cytoplasmic methanemonooxygenase—localized in the cytoplasm.

[0067] As used herein, an “isolated nucleic acid fragment” is a polymerof RNA or DNA that is single- or double-stranded, optionally containingsynthetic, non-natural or altered nucleotide bases. An isolated nucleicacid fragment in the form of a polymer of DNA may be comprised of one ormore segments of cDNA, genomic DNA or synthetic DNA.

[0068] “Gene” refers to a nucleic acid fragment that expresses aspecific protein, including regulatory sequences preceding (5′non-coding sequences) and following (3′ non-coding sequences) the codingsequence. “Native gene” refers to a gene as found in nature with its ownregulatory sequences. “Chimeric gene” refers to any gene that is not anative gene, comprising regulatory and coding sequences that are notfound together in nature. Accordingly, a chimeric gene may compriseregulatory sequences and coding sequences that are derived fromdifferent sources, or regulatory sequences and coding sequences derivedfrom the same source, but arranged in a manner different than that foundin nature. “Endogenous gene” refers to a native gene in its naturallocation in the genome of an organism. A “foreign” gene refers to a genenot normally found in the host organism, but that is introduced into thehost organism by gene transfer. Foreign genes can comprise native genesinserted into a non-native organism, or chimeric genes. A “transgene” isa gene that has been introduced into the genome by a transformationprocedure.

[0069] The term “percent identity”, as known in the art, is arelationship between two or more polypeptide sequences or two or morepolynucleotide sequences, as determined by comparing the sequences. Inthe art, “identity” also means the degree of sequence relatednessbetween polypeptide or polynucleotide sequences, as the case may be, asdetermined by the match between strings of such sequences. “Identity”and “similarity” can be readily calculated by known methods, includingbut not limited to those described in: Computational Molecular Biology(Lesk, A. M., ed.) Oxford University Press, NY (1988); Biocomputing:Informatics and Genome Projects (Smith, D. W., ed.) Academic Press, NY(1993); Computer Analysis of Sequence Data. Part I (Griffin, A. M., andGriffin, H. G., eds.) Humana Press, NJ (1994); Sequence Analysis inMolecular Biology (von Heinje, G., ed.) Academic Press (1987); andSequence Analysis Primer (Gribskov, M. and Devereux, J., eds.) StocktonPress, NY (1991). Preferred methods to determine identity are designedto give the best match between the sequences tested. Methods todetermine identity and similarity are codified in publicly availablecomputer programs. Sequence alignments and percent identity calculationsmay be performed using the Megalign program of the LASERGENEbioinformatics computing suite (DNASTAR Inc., Madison, Wis.). Multiplealignment of the sequences was performed using the Clustal method ofalignment (Higgins and Sharp (1989) CABIOS. 5:151-153) with the defaultparameters (GAP PENALTY=10, GAP LENGTH PENALTY=10). Default parametersfor pairwise alignments using the Clustal method were KTUPLE 1, GAPPENALTY=3, WINDOW=5 and DIAGONALS SAVED=5.

[0070] Suitable nucleic acid fragments (isolated polynucleotides of thepresent invention) encode polypeptides that are at least about 70%identical, preferably at least about 80% identical to the amino acidsequences reported herein. Preferred nucleic acid fragments encode aminoacid sequences that are about 85% identical to the amino acid sequencesreported herein. More preferred nucleic acid fragments encode amino acidsequences that are at least about 90% identical to the amino acidsequences reported herein. Most preferred are nucleic acid fragmentsthat encode amino acid sequences that are at least about 95% identicalto the amino acid sequences reported herein. Suitable nucleic acidfragments not only have the above homologies but typically encode apolypeptide having at least 50 amino acids, preferably at least 100amino acids, more preferably at least 150 amino acids, still morepreferably at least 200 amino acids, and most preferably at least 250amino acids.

[0071] A nucleic acid molecule is “hybridizable” to another nucleic acidmolecule, such as a cDNA, genomic DNA, or RNA, when a single strandedform of the nucleic acid molecule can anneal to the other nucleic acidmolecule under the appropriate conditions of temperature and solutionionic strength. Hybridization and washing conditions are well known andexemplified in Sambrook, J., Fritsch, E. F. and Maniatis, T. MolecularCloning: A Laboratory Manual, Second Edition, Cold Spring HarborLaboratory Press, Cold Spring Harbor (1989), particularly Chapter 11 andTable 11.1 therein (entirely incorporated herein by reference). Theconditions of temperature and ionic strength determine the “stringency”of the hybridization. Stringency conditions can be adjusted to screenfor moderately similar fragments, such as homologous sequences fromdistantly related organisms, to highly similar fragments, such as genesthat duplicate functional enzymes from closely related organisms.Post-hybridization washes determine stringency conditions. One set ofpreferred conditions uses a series of washes starting with 6×SSC, 0.5%SDS at room temperature for 15 min, then repeated with 2×SSC, 0.5% SDSat 45° C. for 30 min, and then repeated twice with 0.2×SSC, 0.5% SDS at50° C. for 30 min. A more preferred set of stringent conditions useshigher temperatures in which the washes are identical to those aboveexcept for the temperature of the final two 30 min washes in 0.2×SSC,0.5% SDS was increased to 60° C. Another preferred set of highlystringent conditions uses two final washes in 0.1×SSC, 0.1% SDS at 65°C. An additional preferred set of stringent conditions include 0.1×SSC,0.1% SDS, 65° C. and washed with 2×SSC, 0.1% SDS followed by 0.1×SSC,0.1% SDS).

[0072] Hybridization requires that the two nucleic acids containcomplementary sequences, although depending on the stringency of thehybridization, mismatches between bases are possible. The appropriatestringency for hybridizing nucleic acids depends on the length of thenucleic acids and the degree of complementation, variables well known inthe art. The greater the degree of similarity or homology between twonucleotide sequences, the greater the value of Tm for hybrids of nucleicacids having those sequences. The relative stability (corresponding tohigher Tm) of nucleic acid hybridizations decreases in the followingorder: RNA:RNA, DNA:RNA, DNA:DNA. For hybrids of greater than 100nucleotides in length, equations for calculating Tm have been derived(see Sambrook et al., supra, 9.50-9.51). For hybridizations with shorternucleic acids, i.e., oligonucleotides, the position of mismatchesbecomes more important, and the length of the oligonucleotide determinesits specificity (see Sambrook et al., supra, 11.7-11.8). In oneembodiment the length for a hybridizable nucleic acid is at least about10 nucleotides. Preferable a minimum length for a hybridizable nucleicacid is at least about 15 nucleotides; more preferably at least about 20nucleotides; and most preferably the length is at least 30 nucleotides.Furthermore, the skilled artisan will recognize that the temperature andwash solution salt concentration may be adjusted as necessary accordingto factors such as length of the probe.

[0073] The term “sequence analysis software” refers to any computeralgorithm or software program that is useful for the analysis ofnucleotide or amino acid sequences. “Sequence analysis software” may becommercially available or independently developed. Typical sequenceanalysis software will include but is not limited to the GCG suite ofprograms (Wisconsin Package Version 9.0, Genetics Computer Group (GCG),Madison, Wis.), BLASTP, BLASTN, BLASTX (Altschul et al., J. Mol. Biol.215:403-410 (1990), and DNASTAR (DNASTAR, Inc. 1228 S. Park St. Madison,Wis. 53715 USA), and the FASTA program incorporating the Smith-Watermanalgorithm (W. R. Pearson, Comput. Methods Genome Res., [Proc. Int.Symp.] (1994), Meeting Date 1992, 111-20. Editor(s): Suhai, Sandor.Publisher: Plenum, New York, N.Y.). Within the context of thisapplication it will be understood that where sequence analysis softwareis used for analysis, that the results of the analysis will be based onthe “default values” of the program referenced, unless otherwisespecified. As used herein “default values” will mean any set of valuesor parameters which originally load with the software when firstinitialized.

[0074] Standard recombinant DNA and molecular cloning techniques usedhere are well known in the art and are described by Sambrook, J.,Fritsch, E. F. and Maniatis, T., Molecular Cloning: A Laboratory Manual,Second Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor,N.Y. (1989) (hereinafter “Maniatis”); and by Silhavy, T. J., Bennan, M.L. and Enquist, L. W., Experiments with Gene Fusions, Cold Spring HarborLaboratory Cold Press Spring Harbor, N.Y. (1984); and by Ausubel, F. M.et al., Current Protocols in Molecular Biology, published by GreenePublishing Assoc. and Wiley-Interscience (1987).

[0075] The present invention provides a unique methanotrophic bacterialstrain, useful for the production of a variety materials from C1 carbonsources such as methane and methanol. The strain is referred to hereinas Methylomonas 16a, and is characterized by rapid doubling time, highyield and the presence of genes encoding both the Entner-Douderoffcarbon pathway as well as the Embden-Meyerhof pathway, allowing forversatility in carbon flux management and higher efficiency of carbonincorporation. The strain has been shown to produce a variety of foodand feed products such as single cell protein, exopolysaccharide andstarch. The strain has particularly high value in the production of foodand feed materials as it is possible to manipulate the variousconcentrations of protein, carbohydrate and starch all within the sameorganism. This capability will permit strains to be uniquely tailoredfor individual specific food and feed applications. Additionally thestrain has demonstrated utility in the production of terpenoid andcarotenoid compounds, useful as pigments and as monomers in polymericmaterials.

[0076] Isolation of Methylomonas 16a

[0077] The original environmental sample containing Methylomonas 16a wasobtained from pond sediment. The pond sediment was inoculated directlyinto a defined mineral medium under 25% methane in air. Methane was usedas the sole source of carbon and energy. Growth was followed until theoptical density at 660 nm was stable, whereupon the culture wastransferred to fresh medium such that a 1:100 dilution was achieved.After 3 successive transfers with methane as the sole carbon and energysource the culture was plated onto defined minimal medium agar andincubated under 25% methane in air. Many methanotrophic bacterialspecies were isolated in this manner. However, Methylomonas 16a wasselected as the organism to study due to the rapid growth of colonies,large colony size, its ability to grow on minimal media, and pinkpigmentation indicative of an active biosynthetic pathway forcarotenoids.

[0078] Methanotrophs are classified into three metabolic groups (“TypeI”, “Type X” or “Type II”) based on the mode of carbon incorporation,morphology, % GC content and the presence or absence of key specificenzymes. Example 4, Table 2 shows key traits determined for Methylomonas16a in relation to the three major groupings of methanotrophs. Thestrain clearly falls into the Type I grouping based on every trait, withthe exception of nitrogen fixation. It is generally well accepted thatthese organisms do not fix nitrogen. Therefore, Methylomonas 16a appearsunique in this aspect of nitrogen metabolism.

[0079] 16SrRNA extracted from the strain was sequenced and compared toknown 16SrRNAs from other microorganisms. The data showed 96% identityto sequences from Methylomonas sp. KSP III and Methylomonas sp. strainLW13. Based on this evidence, as well as the other physiological traitsdescribed in Table 2 (Example 4), it was concluded that the strain was amember of the genus Methylomonas.

[0080] Metabolic and Physiological Characterization of Methylomonas 16a

[0081] Carbon Metabolism: The present methanotrophic bacterial strain,Methylomonas 16a, converts methane to methanol via a methanemonooxygenase as the first step in carbon utilization. The methanemonooxygenase present in the strain is a particulate, as opposed to asoluble, monooxygenase. Particulate methane monooxygenases (pMMO) arewell known in the art (Murrell et al., Arch. Microbiol. (2000),173(5-6), 325-332) and many have been isolated and sequenced. pMMO's arecharacterized by their narrow substrate specificity as opposed to sMMO'swhich are less discriminating. For this reason the pMMO enzyme isfavored for the production of bulk chemicals since the sMMO is likely tomodify many of the chemical intermediates needed for the efficientproduction of a specific product.

[0082] The gene and gene product corresponding to the pMMO isolated fromthe present strain have been sequenced and functionally identified onthe basis of homology comparisons to sequences in publicly availabledatabases. The instant sequence is highly homologous to that isolatedfrom Methylococcus capsulatus (GenBank B57266).

[0083] The present strain contains several anomalies in the carbonutilization pathway. For example, based on genome sequence data, thestrain is shown to contain genes for two pathways of hexose metabolism.The Entner-Douderoff Pathway utilizing the keto-deoxy phosphogluconatealdolase enzyme is present in the strain. Is generally well acceptedthat this is the operative pathway in obligate methanotrophs. Alsopresent, however, is the Embden-Meyerhof pathway which utilizes thefructose bisphosphate aldolase enzyme. It is well known that thispathway is either not present or not operative in obligatemethanotrophs. Energetically, the latter pathway is most favorable andallows greater yield of biologically useful energy, ultimately resultingin greater yield production of cell mass and other cell mass-dependentproducts in Methylomonas 16a. The activity of this pathway in thepresent 16a strain has been confirmed through microarray data andbiochemical evidence measuring the reduction of ATP. Although the 16astrain has been shown to possess both the Embden-Meyerhof and theEntner-Douderoff pathway enzymes the data suggests that theEmbden-Meyerhof pathway enzymes are more strongly expressed than theEntner-Douderoff pathway enzymes. This result is surprising and counterto existing beliefs concerning the glycolytic metabolism ofmethanotrophic bacteria. Applicants have discovered other methanotrophicbacteria having this characteristic, including for example, Methylomonasclara and Methylosinus sporium. It is likely that this activity hasremained undiscovered in methanotrophs due to the lack of activity ofthe enzyme with ATP, the typical phosphoryl donor for the enzyme in mostbacterial systems.

[0084] A particularly novel and useful feature of the Embden-Meyerhofpathway in strain 16a is that the key phosphofructokinase step ispyrophosphate dependent instead of ATP dependent. This feature adds tothe energy yield of the pathway by using pyrophosphate instead of ATP(Example 6). Because of its significance in providing an energeticadvantage to the strain, this gene in the carbon flux pathway isconsidered diagnostic for the present strain.

[0085] Comparison of the pyrophosphate dependent phosphofructokinasegene sequence (SEQ ID NO:5) and deduced amino acid sequence (SEQ IDNO:6) to public databases reveals that the most similar known sequencesis about 63% identical to the amino acid sequence reported herein over alength of 437 amino acid using a Smith-Waterman alignment algorithm (W.R. Pearson, Comput. Methods Genome Res., [Proc. Int. Symp.] (1994),Meeting Date 1992, 111-20. Editor(s): Suhai, Sandor. Publisher: Plenum,New York, N.Y.). More preferred amino acid fragments are at least about80%-90% identical to the sequences herein. Most preferred are nucleicacid fragments that are at least 95% identical to the amino acidfragments reported herein. Similarly, preferred pyrophosphate dependentphosphofructokinase encoding nucleic acid sequences corresponding to theinstant gene are those encoding active proteins and which are at least80% identical to the nucleic acid sequences of reported herein. Morepreferred pyrophosphate dependent phosphofructokinase nucleic acidfragments are at least 90% identical to the sequences herein. Mostpreferred are pyrophosphate dependent phosphofructokinase nucleic acidfragments that are at least 95% identical to the nucleic acid fragmentsreported herein.

[0086] Accordingly the invention provides a high growth methanotrophicbacterial strain which:

[0087] (a) grows on a C1 carbon substrate selected from the groupconsisting of methane and methanol; and

[0088] (b) comprises a functional Embden-Meyerhof carbon pathway, saidpathway comprising a gene encoding a pyrophosphate dependentphosphofructokinase enzyme, the gene selected from the group consistingof:

[0089] (a) an isolated nucleic acid molecule encoding the amino acidsequence as set forth in SEQ ID NO:6;

[0090] (b) an isolated nucleic acid molecule that hybridizes with (a)under the following hybridization conditions: 0.1×SSC, 0.1% SDS, 65° C.and washed with 2×SSC, 0.1% SDS followed by 0.1×SSC, 0.1% SDS;

[0091] (c) an isolated nucleic acid molecule comprising a firstnucleotide sequence encoding a polypeptide of at least 437 amino acidsthat has at least 63% identity based on the Smith-Waterman method ofalignment when compared to a polypeptide having the sequence as setforth in SEQ ID NO:6; and

[0092] (d) an isolated nucleic acid molecule that is complementary to(a), (b) or (c).

[0093] Methane and methanol are the only substrates shown to supportgrowth of Methylomonas 16a. The strain is grown on defined mediumwithout the addition of complex growth factors. Methanol utilization isreported to typically require “adaptation” and growth on methanolconcentration ranging from 0.1% to 3% is also reported as “variable”.Methylomonas 16a was shown to grow on methanol concentrations as high as600 mM (2.4%) without adaptation and with good yield. (FIG. 2).

[0094] In methanotrophic bacteria methane is converted to biomoleculesvia a cyclic set of reactions known as the ribulose monophosphatepathway or RuMP cycle. This pathway is comprised of three phases, eachphase being a series of enzymatic steps. The first step is “fixation” orincorporation of C-1 (formaldehyde) into a pentose to form a hexose orsix carbon sugar. This occurs via a condensation reaction between a 5carbon sugar (pentose) and formaldehyde and is catalyzed by hexulosemonophosphate synthase. The second phase is termed “cleavage” andresults in splitting of that hexose into two 3 carbon molecules. One ofthose three carbon molecules is recycled back through the RuMP pathwayand the other 3 carbon fragment is utilized for cell growth. Inmethanotrophs and methylotrophs the RuMP pathway may occur as one ofthree variants. However, only two of these variants are commonly found:the FBP/TA (fructose bisphosphotase/Transaldolase) or the KDPG/TA (ketodeoxy phosphogluconate/transaldolase) pathway. (Dijkhuizen L., G. E.Devries. The physiology and biochemistry of aerobic methanol-utilizinggram negative and gram positive bacteria. In: Methane and MethanolUtilizers 1992, ed. Colin Murrell and Howard Dalton. Plenum Press, NY).

[0095] The present strain is unique in the way it handles the “cleavage”steps as genes were found that carry out this conversion via fructosebisphosphate as a key intermediate. The genes for fructose bisphosphatealdolase and transaldolase were found clustered together on one piece ofDNA. Secondly the genes for the other variant involving the keto deoxyphosphogluconate intermediate were also found clustered together.Available literature teaches that these organisms (methylotrophs andmethanotrophs) rely solely on the KDPG pathway and that theFBP-dependent fixation pathway is utilized by facultative methylotrophs(Dijkhuizen et al., supra). Therefore the latter observation isexpected, whereas the former is not. The finding of the FBP genes in anobligate methane utilizing bacterium is both surprising and suggestiveof utility. The FBP pathway is energetically favorable to the hostmicroorganism due to the fact that less energy (ATP) is utilized than isutilized in the KDPG pathway. Thus organisms that utilize the FBPpathway may have an energetic advantage and growth advantage over thosethat utilize the KDPG pathway. This advantage may also be useful forenergy-requiring production pathways in the strain. By using thispathway, a methane-utilizing bacterium may have an advantage over othermethane utilizing organisms as production platforms for either singlecell protein or for any other product derived from the flow of carbonthrough the RuMP pathway.

[0096] Accordingly the present invention provides a Methylomonas havingtwo distinct carbon flux pathways, comprising genes and gene products asset forth in SEQ ID NO:1-20, and encoding both a pyrophosphate dependentphosphofructokinase pyrophosphate and a keto-deoxy phosphogluconate(KDPG) aldolase. Comparison of the KDPG aldolase gene sequence (SEQ IDNO:19) and deduced amino acid sequence (SEQ ID NO:20) to publicdatabases reveals that the most similar known sequences is about 59%identical to the amino acid sequence of reported herein over a length of212 amino acid using a Smith-Waterman alignment algorithm (W. R.Pearson, supra). More preferred amino acid fragments are at least about80%-90% identical to the sequences herein. Most preferred are nucleicacid fragments that are at least 95% identical to the amino acidfragments reported herein. Similarly, preferred KDPG aldolase encodingnucleic acid sequences corresponding to the instant gene are thoseencoding active proteins and which are at least 80% identical to thenucleic acid sequences of reported herein. More preferred KDPG aldolasenucleic acid fragments are at least 90% identical to the sequencesherein. Most preferred are KDPG aldolase nucleic acid fragments that areat least 95% identical to the nucleic acid fragments reported herein.

[0097] It is thus an object of the invention to provide a high growthmethanotrophic bacterial strain having the ability to grow exclusivelyon either methane or methanol, comprising a functional Embden-Meyerhofcarbon pathway, said pathway comprising a gene encoding a pyrophosphatedependent phosphofructokinase enzyme and at least one gene encoding aketo-deoxy phosphogluconate aldolase enzyme, selected from the groupconsisting of:

[0098] (a) an isolated nucleic acid molecule encoding the amino acidsequence as set forth in SEQ ID NO:20;

[0099] (b) an isolated nucleic acid molecule that hybridizes with (a)under the following hybridization conditions: 0.1×SSC, 0.1% SDS, 65° C.and washed with 2×SSC, 0.1% SDS followed by 0.1×SSC, 0.1% SDS;

[0100] (c) an isolated nucleic acid molecule comprising a firstnucleotide sequence encoding a polypeptide of at least 212 amino acidsthat has at least 59% identity based on the Smith-Waterman method ofalignment when compared to a polypeptide having the sequence as setforth in SEQ ID NO:20; and

[0101] (d) an isolated nucleic acid molecule that is complementary to(a), (b) or (c).

[0102] In addition to the pyrophosphate dependent phosphofructokinaseenzyme and keto-deoxy phosphogluconate aldolase enzyme, the straincomprises other carbon flux genes including an FBP aldolase,phosphoglucomutase, pyrophosphate dependent phosphofructokinasepyrophosphate, 6-Phosphogluconate dehydratase, and a glucose-6phosphate-1 dehydrogenase. The phosphoglucomutase is responsible for theinterconversion of glucose-6-phosphate to glucose-1-phosphate, whichfeeds into either the Entner-Douderoff or Embden-Meyerhof carbon fluxpathways. As shown in FIG. 3, fructose-6-phosphate may be convert toeither glucose-6-phosphase by glucose phosphate isomerase(Entner-Douderoff) or to fructose-1,6-bisphosphate (FBP) by aphosphofructokinase (Embden-Meyerhof). Following the Embden-Meyerhofpathway, FBP is then taken to two three-carbon moieties(dihydroxyacetone and 3-phosphoglyceraldehyde) by the FBP aldolase.Returning to the Entner-Douderoff system, glucose-6-phosphate is takento 6-phosphogluconate by a glucose-6-phosphate dehydrogenase which issubsequently taken to 2-keto-3-deoxy-6-phosphogluconate (KDPG) by a 6phosphogluconate dehydratase. The KDPG is then converted to twothree-carbon moieties (pyruvate and 3-phosphoglyceraldehyde) by a KDPGaldolase. Thus the Embden-Meyerhof and Entner-Douderoff pathways arerejoined at the level of 3-phosphoglyceraldehyde.

[0103] Identification of High Growth Methanotrophic Bacteria

[0104] Although the present 16a strain has been isolated fortuitously,it is contemplated that the present teaching will enable the generalidentification and isolation of similar strains. For example, the keycharacteristics of the present high growth strain are that it is anobligate methanotroph, using only either methane or methanol as a solecarbon source; and it possesses a functional Embden-Meyerhof pathway,and particularly a gene encoding a pyrophosphate dependentphosphofructokinase. Methods for the isolation of methanotrophs arecommon and well known in the art (See for example Thomas D. Brock inBiotechnology: A Textbook of Industrial Microbiology, Second Edition(1989) Sinauer Associates, Inc., Sunderland, Mass., or Deshpande, MukundV., Appl. Biochem. Biotechnol., 36, 227, (1992)). Similarlypyrophosphate dependent phosphofructokinase has been well characterizedin mammalian systems and assay methods have been well developed (see forexample Schliselfeld et al. Clin. Biochem. (1996), 29(1), 79-83; Clarket al., J. Mol. Cell. Cardiol. (1980), 12(10), 1053-64). Thecontemporary microbiologist will be able to use these techniques toidentify the present high growth strain.

[0105] The specific strain of the present invention possesses a specificpyrophosphate dependent phosphofructokinase having the amino acidsequence as set forth in SEQ ID NO:6. The present strain may be furthercharacterized by analyzing a methanotrophic bacterial strain for thepresence of the gene encoding this enzyme.

[0106] It is therefore an object of the invention to provide a method ofidentifying a high growth methanotrophic bacterial strain comprising:

[0107] (a) growing a sample suspected of containing a high growthmethanotrophic bacterial strain on a suitable growth medium in thepresence of methane as a sole carbon source;

[0108] (b) identifying colonies that grow on the conditions of step (a);

[0109] (c) analyzing the colonies identified in step (b) for thepresence of pyrophosphate dependent phosphofructokinase activity.

[0110] Growth Characteristics: The presence of the above mentionedcarbon flux characteristics was previously unknown in methanotrophicbacteria and may explain the rapid growth rate and the increased carbonconversion efficiency of this strains and other strains possessing thispathway, relative to strain that do not have this pathway. The presentMethylomonas 16a has been shown to grow on methane with a doubling timeof only 2.5 h. This is a very high growth rate and is an obviousadvantage for commercial use as well as for the genetic manipulationsperformed in development of the strain. Additionally, Methylomonas hasno requirement for organic growth factors such as yeast extract or othercostly fermentation additives. The strain requires only methane ormethanol, inorganic minerals, oxygen and water for optimum growth,giving the present strain an advantage for large scale growth at lowcost.

[0111] Particularly noteworthy is the high yield of the present strain.Yield is defined herein as the amount of cell mass produced per gram ofcarbon substrate metabolized. The present strain has shown the abilityto produce greater than 0.8 and preferably greater than 1.0 grams ofcell mass per gram of methane metabolized. Similarly the present strainhas shown the ability to produce greater than 0.30 and preferablygreater than 0.45, more preferably greater than 0.5 cell mass per gramof methanol metabolized.

[0112] Carbon conversion efficiency is another measure of how muchcarbon is assimilated into cell mass. Carbon conversion efficiency isexpressed in units of g/mol methane (1 g dry wt/g methane)/g/molbiomass. Carbon conversion efficiency is calculated assuming a biomasscomposition of CH₂ O_(0.5) N_(0.25). The present strain will have aparticularly high carbon conversion efficiency where an efficiency ofgreater than 40 is common, an efficiency of greater than 50 ispreferred, a conversion of greater than 65 is highly preferred and anefficient of greater than 70 is most preferred.

[0113] Methanol Utilization: Methylomonas 16a is shown to grow atmethanol concentrations as high as 600 mM. Typically methanol can betoxic at these concentrations to some methanotrophic bacteria.Methylomonas 16a can tolerate up to about 2.4% methanol which is at theupper end of the known spectrum of methanol tolerance for methanotrophicbacteria (Green, Peter, Taxonomy of Methylotrophic Bacteria. In: Methaneand Methanol Utilizers (Biotechnology Handbooks 5) J. Colin Murrell andHoward Dalton eds., 1992 Pleanum Press NY, pp 23-84). This feature againallows for much lower capital costs in reactor design since tolerancefor methanol is higher necessitating reactors with fewer mixing ports(i.e. lower construction costs). This issue (high reactor costs due tomixing requirements to overcome methanol toxicity) is a major drawbackto growth of methanotrophic bacteria on methanol.

[0114] Glycogen Production: Methylomonas 16a has been shown to producein excess of 50% of its weight as glycogen during active growth onmethanol and significant amounts of glycogen during active (non-stressassociated) growth on methane. This aspect is useful for the productionof mixtures of protein and carbohydrate to serve a wider array of animalfeed nutritional needs as compared to other obligate methanotrophsproducing only protein as the sole product. Alternatively, this traitenables Methylomonas 16a to serve as a host strain for the production ofglycogen from methane or methanol. Furthermore, internal hexosemetabolism is clearly occurring in Methylomonas 16a. Thus the organismcan serve as host for the production of chemical products typicallyconsidered to be only produced by carbohydrate metabolism. Accordinglythe invention provides a Methylomonas strain having the ability toproduce in excess of 50% of its weight of glycogen when grown onmethanol, where about 20% to about 40% is typical.

[0115] Pigment and Terpenoid Production: The present Methylomonas strainis useful for the production of a variety of pigments and particularlythe isoprenoid pigments. This class of pigments are known to have stronglight absorbing properties and are derived from the head to tailcondensation of 5, 10, 15, 20, 25, 30 or 40 carbon isoprene chains. Onespecific pigment identified in the present strain is a C-30 carotenoid.The content of this pigment is very high in the cell and is indicativeof naturally high carbon flow through the isoprenoid pathway. Thisaspect provides the basis for viewing the isoprenoid pathway as a“backbone production pathway” for isoprenoid-derived products. It iscontemplated for example that high value carotenoids such asastaxanthin, α-carotene, canthaxanthin, and lutein may be produced bythe instant organism.

[0116] Additionally the present strain is expected to have the abilityto produce various isoprenoid compounds. Isoprenoids are an extremelylarge and diverse group of natural products that have a commonbiosynthetic origin based on a single metabolic precursor known asisopentenyl diphosphate (IPP). The group of natural products known asisoprenoids includes all substances that are derived biosyntheticallyfrom the 5-carbon compound isopentenyl diphosphate. Isoprenoid compoundsare also referred to as “terpenes” or “terpenoids”, which is the termused in the designation of the various classes of these examples(Spurgeon and Porter, Biosynthesis of Isoprenoid Compounds, pp 346, AWiley-Interscience Publication (1981)). Isoprenoids are ubiquitouscompounds found in all living organisms. Some of the well-known examplesof isoprenoids are steroids (triterpenes), carotenoids (tetraterpenes),and squalene, just to name a few.

[0117] The biosynthesis of such compounds typically involve the enzymeisopentenyl pyrophosphate and are formed by the head to tailcondensation of isoprene units which may be of 5, 10, 15, 20, 30 or 40carbons in length.

[0118] It is contemplated that other, related, small cyclic moleculessuch as limonene, menthol and geraniol may be produced in the presentstrain via the introduction of the appropriate plant-derived terpenesynthases. Thus the isoprenoid pathway may be viewed as a platformpathway for production of complex cyclic and unsaturated molecules frommethane or methanol. This capability is unique to biology, purelychemical processes cannot convert C-1 compounds to cyclic molecules withany degree of specificity.

[0119] Many steps in isoprenoid pathways are known. For example, theinitial steps of the alternate pathway involve the condensation of3-carbon molecules (pyruvate and C1 aldehyde group, D-glyceraldehyde3-Phosphate), to yield a 5-carbon compound(D-1-deoxyxylulose-5-phosphate). Lois et al. has reported a gene, dxs,that encodes D-1-deoxyxylulose-5-phosphate synthase (DXS) that catalyzesthe synthesis of D-1-deoxyxylulose-5-phosphate in E. coli (Proc. Natl.Acad. Sci. USA 95: 2105-2110 (1998)).

[0120] Next, the intramolecular rearrangement ofD-1-deoxyxylulose-5-phosphate occurs by an unspecified reduction processfor the formation of 2-C-methyl-D-erythritol-4-phosphate. One of theenzymes involved in the reduction process isD-1-deoxyxylulose-5-phosphate reductoisomerase (DXR). Takahashi et al.reported the dxr gene product catalyzes the formation of2-C-methyl-D-erythritol-4-phosphate in the alternate pathway in E. coli(Proc. Natl. Acad. Sci. USA 95: 9879-9884 (1998)).

[0121] Steps converting 2-C-methyl-D-erythritol-4-phosphate toisopentenyl monophosphate are not well characterized although some stepsare known. 2-C-methyl-D-erythritol-4-phosphate is converted into4-diphosphocytidyl-2C-methyl-D-erythritol in a cytosine triphosphate(CTP) dependent reaction by the enzyme encoded by non-annotated geneygbP, encoding a 2C-methyl-d-erythritol cytidylyltransferase. Rondich etal. reported a YgbP protein in E. coli that catalyzes the reactionmentioned above (Proc. Natl. Acad. Sci. USA 96:11758-11763 (1999)).Recently, ygbP gene was renamed as ispD as a part of the isp genecluster. The 2 position hydroxy group of4-diphosphocytidyl-2C-methyl-D-erythritol can be phosphorylated in anATP dependent reaction by a 4-diphosphocytidyl-2-C-methylerythritolkinase encoded by the ychB gene. Luttgen et al. has reported a YchBprotein in E. coli that phosphorylates4-diphosphocytidyl-2C-methyl-D-erythritol, resulting in4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate (Proc. Natl. Acad.Sci. USA 97:1062-1067 (2000)). Recently, the ychB gene was renamed asispE as a part of the isp gene cluster.

[0122] Herz et al. reported that the ygbB gene product(2C-methyl-d-erythritol 2,4-cyclodiphosphate synthase) in E. coliconverts 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate to2C-methyl-D-erythritol 2,4-cyclodiphosphate in a CTP dependent reaction.2C-methyl-D-erythritol 2,4-cyclodiphosphate can be further convertedinto carotenoids through the carotenoid biosynthesis pathway (Proc.Natl. Acad. Sci. USA 97:2486-2490 (2000)). Recently, the ygbB gene wasrenamed as ispF as a part of isp gene cluster.

[0123] Both reactions catalyzed by the YgbB and YgbP enzymes are carriedout in CTP dependent manner. Thus CTP synthase plays an important rolein the isoprenoid pathway. PyrG encoded by the pyrG gene in E. coli wasdetermined to encode CTP synthase (Weng et al., J. Biol. Chem.,261:5568-5574 (1986)).

[0124] Following several reactions not yet characterized, isopentenylmonophosphate is formed. Isopentenyl monophosphate is converted to anisopentenyl diphosphate (IPP) by isopentenyl monophosphate kinase enzymeencoded by the ipk gene (Lange and Croteau, Proc. Natl. Acad. Sci. USA96:13714-13719 (1999)).

[0125] Prenyltransferases constitute a broad group of enzymes catalyzingthe consecutive condensation of isopentenyl diphosphate (IPP), resultingin the formation of prenyl diphosphates of various chain lengths.Homologous genes of prenyl transferase have highly conserved regions intheir amino acid sequences. Ohto et al. reported three prenyltransferase genes in cyanobacterium Synechococcus elongatus (Plant Mol.Biol. 40:307-321 (1999)). They are geranylgeranyl (C20) diphosphatesynthase, farnesyl (C15) diphosphate synthase (ispA), and anotherprenyltransferase that can catalyze the synthesis of five prenyldiphosphates of various length.

[0126] Further down in the isoprenoid biosynthesis pathway, more genesare involved in the synthesis of specific isoprenoids. As an example,the crtN gene that was found in Heliobacillus mobilis (Proc. Natl. Acad.Sci. USA 95:14851-14856 (1998)) encodes a diapophytoene dehydrogenasethat is a part of the carotenoid biosynthesis pathway.

[0127] Although some of the genes involved in isoprenoid pathways arewell known, the presence of genes involved in the isoprenoid pathway ofMethylomonas sp. is rare. It is surprising therefore to find all of theabove mentioned genes in the present strain (SEQ ID NO:61-SEQ ID NO:78).Tgus suggests that the present strain will be useful for the productionof a variety of terpenoids. Accordingly the invention provides aMethylomonas strain having the genes and gene products as set forth inSEQ ID NO:61-SEQ ID NO:78, encoding a D-1-deoxyxylulose-5-phosphatesynthase, a D-1-deoxyxylulose-5-phosphate reductoisomerase,2C-methyl-d-erythritol 2,4-cyclodiphosphate synthase, a2C-methyl-d-erythritol cytidylyltransferase, a CTP synthase, aGeranyltranstransferase (also farnesyl-diphosphate synthase), a4-diphosphocytidyl-2-C-methylerythritol kinase, and a diapophytoenedehydrogenase.

[0128] Production of Single Cell Protein: The present strain is usefulfor the production of single cell protein (SCP) which has value in thefood and feed industries. Methods for the use of methanotrophs asproduction platforms for the production of SCP are well known in the art(see for example U.S. Pat. No. 4,795,708; Shojaosadati et al., Amirkabir(1996), 8(30), 33-41). The present strain is well suited for thisapplication due to its advantages in carbon flux and reduced oxygenconsumption in the presence of a nitrogen source. The strain is wellsuited for the production of single cell protein under either aerobic oranaerobic conditions.

[0129] The present strain compares favorably with other known strains,producing up to about 1.3 g protein/dry weight g methane and up to about0.45 g protein/dry weight g methanol.

[0130] Production of exopolysaccharides: Polysaccharides are sugarpolymers that have been used widely as a thickener in food and non-foodindustries (Sanford et al. Pure & Appl. Chem. 56: 879-892 (1984);Sutherland, Trends Biotechnol, 16(1): 41-6 (1998)). They can be found infood products such as salad dressing, jam, frozen food, bakery products,canned food and dry food. Many other applications include suspendingagents for pesticides, paints and other coating agents. They can act asflocculent, binders, film-formers, lubricants and friction reducers.Furthermore, exopolysaccharides are commonly used in the oil field foroil recovery.

[0131] Traditionally, industrially useful polysaccharides have beenderived from algal and plant sources. Over the past decadepolysaccharides derived from microbes have been found increased usage(Sanford et al. Pure & Appl. Chem. 56: 879-892 (1984); Sutherland,Trends Biotechnol, 16(1): 41-6 (1998)).

[0132] Many other genes involved in exopolysaccharide biosynthesis havebeen characterized or sequenced from other organisms. The epsB geneencodes the EpsB protein that is probably involved in polymerizationand/or export of EPS, and has been sequenced in Ralstonia sola (Huang etal, Mol. Microbiol. 16: 977-989 (1995)). The espM gene encoding the EspMprotein has been found in the esp gene cluster from Streptococcusthermophilus (Stingele et al, J. Bacteiol. 178: 1680-1690 (1996)).Another putative polysaccharide export protein, WZA, is identified in E.coli. (Blattner et al., Science 277: 1453-1474 (1997)). Finally, theepsV gene encodes the EpsV protein, a transferase which transfers thesugar to polysaccharide intermediates, and it has also been sequenced inStreptococcus thermophilus (Bourgoin et al., Plasmid 40: 44-49 (1998);Bourgoin, F., et al., Gene 233:151-161 (1999)).

[0133] In spite of the abundance of information regarding genes encodingmicrobial exopolysaccharides, no genes involved in this pathway havebeen isolated or characterized from C1 utilizing organisms, such asMethylomonas. As noted above, microbial exopolysaccharides have avariety of uses and it would be an advantage to synthesize this materialfrom an abundant and inexpensive carbon source such as methane.

[0134] Surprisingly, the present Methylomonas 16a has been shown toproduce extrapolysaccharides at high levels. The genes encoding therelevant polysaccharide synthesis pathways have been isolated andcharacterized and are described along with their gene products in SEQ IDNO:21-SEQ ID NO:38.

[0135] Accordingly, the present invention provides a Methylomonas strainhaving the ability to synthesize exopolysaccharides and having genesencoding the ugp, gumD, wza, epsB, epsM, waaE, epsV, gumH and glycosyltransferase proteins associated with microbial polysaccharidebiosynthesis.

[0136] Denitrification: The presence of denitrification enzymes inobligate methanotrophs is unknown. The present strain contains a pathwaycomprised of genes and gene products as set forth in SEQ ID NO:39-SEQ IDNO:60. A novel feature of the present Methylomonas 16a is the ability toutilize a nitrogen source at low oxygen tensions as an additional“electron sink” for reducing equivalents derived from methane ormethanol. Nitrogen sources may include, but are not limited to, nitrite,nitrate, ammonium and dinitrogen. The strain is shown to reduce nitrateor nitrite to nitrous oxide which is a gaseous end-product. The utilityin this process is that nitrate is very soluble as well as inexpensiveand use of nitrate mitigates against the high energy requirement formaintaining dissolved oxygen in the process. In fact, nitrate isutilized as an accessory oxidant in some waste water treatment systems(Koch, Gerhard; Siegrist, Hansruedi Verbandsber.-Verb. Schweiz.Abwasser-Gewaesserschutzfachleute (1998), 522 (Optimierungsmassnahmenbei Stark Belasteten Belebungsanlagen), 33-48).

[0137] In non-methanotrophic denitrifiers, the microbial process knownas denitrification is catalyzed by a series of enzymes which togetherreductively convert nitrate to gaseous dinitrogen. The steps andintermediates in the process as shown below, together with the enzymenames and gene designations define the scope of the process underconsideration.

[0138] 1. NO₃→NO₂ Respiratory nitrate reductase (Nar genes).

[0139] 2. NO₂→NO Respiratory nitrite reductase (Nir genes)

[0140] 3. NO→N₂O Nitric oxide reductase (Nor genes)

[0141] 4. N₂O→N₂ Nitrous oxide reductase (Nos genes)

[0142] Ecologically, the result of these processes is removal ofnitrogen from soils (denitrification). However, nitrate can also beviewed as a supplemental or alternative oxidant to oxygen. This is dueto the very positive redox potential of the denitrification process.

[0143] A second major microbial process is referred to as nitrificationand that is comprised of the following set of reactions, enzymes andgenes.

[0144] 1. NH₄→NH₂OH Ammonia monooxygenase (amo genes)

[0145] 2. NH₂OH→NO₂ (Hydroxylamine oxidoreductase)

[0146] 3. NO₂→NO₃ (Nitrite oxidase)

[0147] Nitrification is an oxidative process generating nitrate in soilswhereas denitrification is a reductive process depleting nitrate insoils.

[0148] It is well known that obligatory methanotrophic bacteria belongto the group of nitrifying bacteria. This is due to the ability ofmethane monooxygenase which is found in all obligate methanotrophs tooxygenate ammonia to form hydroxylamine in a reaction identical to thatof ammonia monooxygenase and analogous to methane oxygenation to formmethanol. The hydroxylamine is then further metabolized enzymatically tonitrite. Nitrite oxidation to nitrate can occur enzymatically orspontaneously in air via chemical oxidation. However methanotrophicbacteria have been indirectly associated with denitrification by virtueof their association with denitrifying bacteria such as Hyphomicrobiumspecies (Amaral, J. A. Archambault, C. S. R. Richards, R. Knowles 1995.FEMS Microbiology Ecology 18 289-298). The respiratory processesdescribed above are distinct from the reduction of nitrate or nitritefor cellular assimilation. The former respiratory process is energyyielding whereas the latter assimilatory process provides nitrogen forincorporation into cellular mass. The assimilatory process relies uponpyridine nucleotide linked nitrate or nitrite reductases. These enzymesare widely found in nature including the methanotrophic bacteria. Growthof methanotrophs on nitrate as a sole nitrogen source for biosynthesisis well known in the existing literature (Hanson R. S. A. I. Netrusov,K. Tsuji. 1992. The obligate methanotrophic bacteria Methylococcus,Methylomonas, and Methylosinus. In: The Prokaryotes 2^(nd) ed. Ch 18. Pp2350-2363, A. Balows, H. G. Truper, M. Dworkin, W. Harder, K-H Schleifereds. Springer Verlag).

[0149] The functionality of the genes described herein (SEQ ID NO:39-SEQID NO:60) lie in the respiratory reduction of nitrate or nitrite togaseous N₂O. All genes required to perform this function have been shownto be present in Methylomonas 16a both by sequence analysis andphysiological reduction of nitrogen containing compounds. Additionallythe genes encoding enzymes necessary for the biotransformation ofammonia (nitrification) are also present.

[0150] The advantages to the presence of this denitrification capabilityin an obligate methanotroph are at least two fold:

[0151] 1. Nitrate may replace or supplement oxygen as an electronacceptor needed for growth. This can be advantageous for large scalecost-effective cultivation with highly reduced feedstocks that requireexcessive oxygen demand leading to excessive costs for mass-transfer ofgaseous oxygen into solution.

[0152] 2. Methanotrophic denitrification may be used to remove solublenitrates from waters or processes where nitrates or other oxygenatednitrogen derivatives are problematic.

[0153] Due to the ability of Methylomonas 16a to convert ammonia tonitrite combined with the ability to convert nitrite to nitrous oxidedemonstrated in the present invention, Methylomonas 16a and othermethanotrophs which efficiently reduce nitrite can be used as agents toremove ammonia from process waters, waste waters, or natural waters oragricultural effluents for the purpose of clean up and detoxification

[0154] Gene Transfer into Methylomonas 16a: Methylomonas 16a has beenshown to accept and express genes form other organisms includingEscherichia coli and yeast. Several plasmid vectors have been identifiedwhich facilitate both gene transfer from a donor organism and expressionof the gene in Methylomonas 16a. Thus the strain can be geneticallyengineered.

[0155] Production of Food and Feed Substrates

[0156] It will be appreciated that the present Methylomonas 16a strainhas the ability to produce, not only proteins, polysaccharides andpigments individually, but may also be engineered to produce a uniquelytailored food or feed product comprising specific quantities anddesirable mixtures of these materials. This characteristic of thepresent strain has significant commercial value.

[0157] For example, different livestock animal types may have differentnutritional requirements in terms of the relative proportions of proteinto carbohydrate. Many carnivorous aquatic fish species, for example,have very high protein requirements. Ruminant livestock, on the otherhand, thrive on higher fiber/carbohydrate diets. Methylomonas 16a hasthe capacity to form large amounts of carbohydrate, under certainconditions, in addition to the cellular protein which is alwaysproduced. Genes involved in gluconeogenesis (glycogen formation) orglycogen degradation might be altered or regulated such that glycogencontent could either be decreased or increased. Thus the composition ofthe crude cell mass could be modulated to target high protein feedmarkets (lower carbohydrate) or alternatively, higher carbohydrate lowerprotein feed markets. The ability to engineer the composition of themicrobe precludes the need to artificially formulateprotein/carbohydrate ratios by exogenous additions.

[0158] Carotenoid pigments play a role in terms of providing colorationfor many aquatic fish and crustacean species as well as providingantioxidant benefit. (Nelis H. J., De Leenheer 1991. J. Appl. Bacterol.70:181-191). Methylomonas 16a, unlike many commercially utilizedmethanotrophs (i.e. Methylococcus capsulatus) has a natural carotenoidpigment production pathway which produces high levels of a pink pigmentthat is similar, but not structurally identical, with such high valuecarotenoids as astaxanthin. Modification of this pathway by addition ofgenes involved in the final steps of astaxanthin synthesis or other highvalue carotenoids will result in the ability of this strain to producethese carotenoids. In this way Methylomonas 16a will be uniquely usefulas an animal feed production strain in which the ratios ofprotein/carbohydrate/pigments may be tailored to suit particularnutritional needs. In this way, Methylomonas may be utilized as a way todeliver higher value components to other sources of plant protein orcarbohydrate and thus circumvent the problem of genetic engineering ofthese plants for the higher value traits.

[0159] Methods of manipulating genetic pathways are common and wellknown in the art. Selected genes in a particular pathway may beupregulated or down regulated by variety of methods. Additionally,competing pathways in the organism may be eliminated or sublimated bygene disruption and similar techniques.

[0160] Once a key genetic pathway has been identified and sequencedspecific genes may be upregulated to increase the output of the pathway.For example, additionally copies of the targeted genes may be introducedinto the host cell on multicopy plasmids such as pBR322. Alternatively,the target genes may be modified so as to be under the control ofnon-native promoters. Where it is desired that a pathway operate at aparticular point in a cell cycle or during a fermentation run, regulatedor inducible promoters may used to replace the native promoter of thetarget gene. Similarly, in some cases the native or endogenous promotermay be modified to increase gene expression. For example, endogenouspromoters can be altered in vivo by mutation, deletion, and/orsubstitution (see, Kmiec, U.S. Pat. No. 5,565,350; Zarling et al.,PCT/US93/03868).

[0161] Alternatively it may be necessary to reduce or eliminate theexpression of certain genes in the target pathway or in competingpathways that may serve as competing sinks for energy or carbon. Methodsof down-regulating genes for this purpose have been explored. Wheresequence of the gene to be disrupted is known, one of the most effectivemethods of gene down regulation is targeted gene disruption whereforeign DNA is inserted into a structural gene so as to disrupttranscription. This can be effected by the creation of genetic cassettescomprising the DNA to be inserted (often a genetic marker) flanked bysequence having a high degree of homology to a portion of the gene to bedisrupted. Introduction of the cassette into the host cell results ininsertion of the foreign DNA into the structural gene via the native DNAreplication mechanisms of the cell. (See for example Hamilton et al.(1989) J. Bacteriol. 171:4617-4622; Balbas et al. (1993) Gene136:211-213; Gueldener et al. (1996) Nucleic Acids Res. 24:2519-2524;and Smith et al. (1996) Methods Mol. Cell. Biol. 5:270-277.)

[0162] Antisense technology is another method of down regulating geneswhere the sequence of the target gene is known. To accomplish this, anucleic acid segment from the desired gene is cloned and operably linkedto a promoter such that the anti-sense strand of RNA will betranscribed. This construct is then introduced into the host cell andthe antisense strand of RNA is produced. Antisense RNA inhibits geneexpression by preventing the accumulation of mRNA which encodes theprotein of interest. The person skilled in the art will know thatspecial considerations are associated with the use of antisensetechnologies in order to reduce expression of particular genes. Forexample, the proper level of expression of antisense genes may requirethe use of different chimeric genes utilizing different regulatoryelements known to the skilled artisan.

[0163] Although targeted gene disruption and antisense technology offereffective means of down regulating genes where the sequence is known,other less specific methodologies have been developed that are notsequence based. For example, cells may be exposed to a UV radiation andthen screened for the desired phenotype. Mutagenesis with chemicalagents is also effective for generating mutants and commonly usedsubstances include chemicals that affect nonreplicating DNA such as HNO₂and NH₂OH, as well as agents that affect replicating DNA such asacridine dyes, notable for causing frameshift mutations. Specificmethods for creating mutants using radiation or chemical agents are welldocumented in the art. See for example Thomas D. Brock in Biotechnology:A Textbook of Industrial Microbiology, Second Edition (1989) SinauerAssociates, Inc., Sunderland, Mass., or Deshpande, Mukund V., Appl.Biochem. Biotechnol., 36, 227, (1992).

[0164] Another non-specific method of gene disruption is the use oftransposoable elements or transposons. Transposons are genetic elementsthat insert randomly in DNA but can be latter retrieved on the basis ofsequence to determine where the insertion has occurred. Both in vivo andin vitro transposition methods are known. Both methods involve the useof a transposable element in combination with a transposase enzyme. Whenthe transposable element or transposon, is contacted with a nucleic acidfragment in the presence of the transposase, the transposable elementwill randomly insert into the nucleic acid fragment. The technique isuseful for random mutageneis and for gene isolation, since the disruptedgene may be identified on the basis of the sequence of the transposableelement. Kits for in vitro transposition are commercially available (seefor example The Primer Island Transposition Kit, available from PerkinElmer Applied Biosystems, Branchburg, N.J., based upon the yeast Ty1element; The Genome Priming System, available from New England Biolabs,Beverly, Mass.; based upon the bacterial transposon Tn7; and the EZ::TNTransposon Insertion Systems, available from Epicentre Technologies,Madison, Wis., based upon the Tn5 bacterial transposable element.

[0165] Within the context of the present invention it may be useful tomodulate the expression of the identified biosynthetic pathways. Forexample, it has been noted that the present Methylomonas 16a comprisesgenes encoding both the Entner-Douderoff and Embden-Meyerhof carbon fluxpathways. Because the Embden-Meyerhof pathway is more energy efficientit may be desirable to over-express the genes in this pathway.Additionally, it is likely that the Entner-Douderoff pathway is acompetitive pathway and inhibition of this pathway may lead to increasedenergy efficiency in the Embden-Meyerhof system. This might beaccomplished by selectively using the above described methods of genedown regulation on the sequence encoding the keto-deoxy phosphogluconatealdolase (SEQ ID NO:9) or any of the other members of theEntner-Douderoff system and upregulating the gene encoding the fructosebisphosphatase aldolase of the Embden-Meyerhof system (SEQ ID NO:50R 7).In this fashion the carbon flux in the present Methylomonas 16a may beoptimized. Additionally, where the present strain has been engineered toproduce specific organic materials such as aromatics for monomerproduction, optimization of the carbon flux pathway will lead toincreased yields of these materials.

[0166] In a similar fashion the genes encoding the key enzymes involvedin isoprenoid or pigment synthesis may be modulated. For example, thepresent invention provides a number of genes encoding key enzymes in theterpenoid pathway leading to the production of pigments and smallerisoprenoid compounds. The isolated genes include the dxs and dsr genes,the ispA, D, E, F, and G genes, the pyrG gene, and crtN genes. Inparticular it may be useful to up-regulate the initial condensation of3-carbon molecules (pyruvate and C1 aldehyde group, D-glyceraldehyde3-Phosphate), to yield the 5-carbon compound(D-1-deoxyxylulose-5-phosphate) mediated by the dxs gene. Alternatively,if it is desired to produce a specific non-pigmented isoprenoid, it maybe desirable to disrupt various genes at the downstream end of thepathway. For example, it may be desirable to use gene disruption orantisense inhibition of the crtN gene (known to encode diapophytoenedehydrogenase) if a smaller, upstream terpenoid is the desired productof the pathway.

[0167] As has been noted, the present strain has the ability to productpolysaccharides in large amounts. This process is governed by a set ofgenes including the ugp gene, gumD and H genes, the epsB, M, and V genesand the waaD gene. In this pathway it may be of particular importance toup-regulate the espB gene involved in polymerization and/or export ofthe polysaccharide, or the epsV gene which controls the transfer ofsugar to polysaccharide intermediates.

[0168] In this fashion the present strain, or a similar strain may beengineered to produce specific compositions of materials or specificcombinations of protein, polysaccharides and pigments for use as a foodand feed product.

EXAMPLES

[0169] The present invention is further defined in the followingExamples. It should be understood that these Examples, while indicatingpreferred embodiments of the invention, are given by way of illustrationonly. From the above discussion and these Examples, one skilled in theart can ascertain the essential characteristics of this invention, andwithout departing from the spirit and scope thereof, can make variouschanges and modifications of the invention to adapt it to various usagesand conditions.

[0170] General Methods

[0171] Standard recombinant DNA and molecular cloning techniques used inthe Examples are well known in the art and are described by Sambrook,J., Fritsch, E. F. and Maniatis, T. Molecular Cloning: A LaboratoryManual; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, (1989)(Maniatis) and by T. J. Silhavy, M. L. Bennan, and L. W. Enquist,Experiments with Gene Fusions, Cold Spring Harbor Laboratory, ColdSpring Harbor, N.Y. (1984) and by Ausubel, F. M. et al., CurrentProtocols in Molecular Biology, pub. by Greene Publishing Assoc. andWiley-Interscience (1987).

[0172] Materials and methods suitable for the maintenance and growth ofbacterial cultures are well known in the art. Techniques suitable foruse in the following examples may be found as set out in Manual ofMethods for General Bacteriology (Phillipp Gerhardt, R. G. E. Murray,Ralph N. Costilow, Eugene W. Nester, Willis A. Wood, Noel R. Krieg andG. Briggs Phillips, eds), American Society for Microbiology, Washington,D.C. (1994)) or by Thomas D. Brock in Biotechnology: A Textbook ofIndustrial Microbiology, Second Edition, Sinauer Associates, Inc.,Sunderland, Mass. (1989). All reagents, restriction enzymes andmaterials used for the growth and maintenance of bacterial cells wereobtained from Aldrich Chemicals (Milwaukee, Wis.), DIFCO Laboratories(Detroit, Mich.), GIBCO/BRL (Gaithersburg, Md.), or Sigma ChemicalCompany (St. Louis, Mo.) unless otherwise specified.

[0173] The meaning of abbreviations is as follows: “h” means hour(s),“min” means minute(s), “sec” means second(s), “d” means day(s), “mL”means milliliters, “L” means liters.

[0174] Microbial Cultivation and Preparation of Cell Suspensions, andAssociated Analyses.

[0175] Methylomonas 16a is typically grown in serum stoppered Wheatonbottles using a gas/liquid ratio of at least 8:1 (i.e. 20 mL of Nitrateliquid media) media in a Wheaton bottle (Wheaton Scientific, WheatonIll.) of 160 mL total volume. The standard gas phase for cultivationcontained 25% methane in air. These conditions comprise growthconditions and the cells are referred to as growing cells. In all casesthe cultures were grown at 30° C. with constant shaking in a Lab-Linerotary shaker unless otherwise specified.

[0176] Cells obtained for experimental purposes were allowed to grow tomaximum optical density (O.D. 660˜1.0). Harvested cells were obtained bycentrifugation in a Sorval RC-5B centrifuge using a SS-34 rotor at 6000rpm for 20 min. These cell pellets were resuspended in 50 mM HEPESbuffer pH 7. These cell suspensions are referred to as washed, restingcells.

[0177] Microbial growth was assessed in all experiments by measuring theoptical density of the culture at 660 nm in an Ultrospec 2000 UVN isspectrophotometer (Pharmacia Biotech, Cambridge England) using a 1 cmlight path cuvet. Alternatively microbial growth was assessed byharvesting cells from the culture medium by centrifugation as describedabove and resuspending the cells in distilled water with a secondcentrifugation to remove medium salts. The washed cells were then driedat 105° C. overnight in a drying oven for dry weight determination.

[0178] Methane concentration was determined as described by Emptage etal. (1997 Env. Sci. Technol. 31:732-734), hereby incorporated byreference.

[0179] Nitrate Medium for Methylomonas 16A

[0180] Nitrate liquid medium, also referred to herein as “definedmedium” was comprised of various salts mixed with solution 1 asindicated below or where specified the nitrate was replaced with 15 mMammonium chloride.

[0181] Solution 1 Composition for 100 fold concentrated stock solutionof trace minerals. Conc. MW (mM) g per L Nitriloacetic acid 191.1 66.912.8 CuCl₂ × 2H₂O 170.48 0.15 0.0254 FeCl₂ × 4H₂O 198.81 1.5 0.3 MnCl₂ ×4H₂O 197.91 0.5 0.1 CoCl₂ × 6H₂O 237.9 1.31 0.312 ZnCl₂ 136.29 0.73 0.1H₃BO₃ 61.83 0.16 0.01 Na₂MoO₄ × 2H₂O 241.95 0.04 0.01 NiCl₂ × 6H₂O 237.70.77 0.184

[0182] Mix the gram amounts designated above in 900 mL of H₂O, adjust topH=7, and add H₂O to an end volume of 1 L. Keep refrigerated.

[0183] Nitrate Liquid Medium: Conc. MW (mM) g per L NaNO₃ 84.99 10  0.85KH₂PO₄ 136.09 3.67 0.5 Na₂SO₄ 142.04 3.52 0.5 MgCl₂ × 6H₂O 203.3 0.980.2 CaCl₂ × 2H₂O 147.02 0.68 0.1 1 M HEPES (pH 7) 238.3 50 mL Solution 110 mL

[0184] Nitrate and Nitrite Assays

[0185] 1 mL samples of cell culture were taken and filtered through a0.2 micron Acrodisc filter to remove cells. The filtrate from this stepcontains the nitrite or nitrate to be analyzed. The analysis wasperformed on a Dionex ion chromatograph 500 system (Dionex, SunnyvaleCalif.) with an AS3500 autosampler. The column used was a 4 mm Ion-PacAS11-HC separation column with an AG-AC guard column and an ATC trapcolumn. All columns are provided by Dionex.

[0186] The mobile phase was a potassium hydroxide gradient from 0 to 50mM potassium hydroxide over a 12 min time interval. Cell temperature was350C with a flow rate of 1 mL/min.

[0187] Gene Isolation and Characterization

[0188] A number of genes encoding specific identifying enzymes wereisolated and sequenced from Methylomonas 16a. These includedistinguishing genes found in the Entner-Douderoff carbon flux pathwaythe Embden-Meyerhof carbon flux pathway, genes encoding adenitrification pathway, genes encoding an isoprenoid synthesis pathway,and genes encoding a pathway for the synthesis of exopolysaccharides.These genes were sequenced and functionally characterized by comparisonof their respective sequences to information in public nucleic acid andprotein databases according to the following procedures.

[0189] Genomic DNA was isolated from Methylomonas 16a according tostandard protocols. Genomic DNA and library construction were preparedaccording to published protocols (Fraser et al The Minimal GeneComplement of Mycoplasma genitalium; Science 270, 1995). A cell pelletwas resuspended in a solution containing 100 mM Na-EDTA pH 8.0, 10 mMtris-HCl pH 8.0, 400 mM NaCl, and 50 mM MgCl2.

[0190] Genomic DNA preparation After resuspension, the cells were gentlylysed in 10% SDS, and incubated for 30 min at 55° C. After incubation atroom temperature, proteinase K was added to 100 μg/mL and incubated at37° C. until the suspension was clear. DNA was extracted twice withtris-equilibrated phenol and twice with chloroform. DNA was precipitatedin 70% ethanol and resuspended in a solution containing 10 mM tris-HCland 1 mM Na-EDTA (TE) pH 7.5. The DNA solution was treated with a mix ofRNAases, then extracted twice with tris-equilibrated phenol and twicewith chloroform. This was followed by precipitation in ethanol andresuspension in TE.

[0191] Library construction 200 to 500 μg of chromosomal DNA wasresuspended in a solution of 300 mM sodium acetate, 10 mM tris-HCl, 1 mMNa-EDTA, and 30% glycerol, and sheared at 12 psi for 60 sec in anAeromist Downdraft Nebulizer chamber (IBI Medical products, Chicago,Ill.). The DNA was precipitated, resuspended and treated with Bal31nuclease. After size fractionation, a fraction (2.0 kb, or 5.0 kb) wasexcised, cleaned and a two-step ligation procedure was used to produce ahigh titer library with greater than 99% single inserts.

[0192] Sequencing A shotgun sequencing strategy approach was adopted forthe sequencing of the whole microbial genome (Fleischmann, Robert et alWhole-Genome Random sequencing and assembly of Haemophilus influenzae RdScience, 269: 1995).

[0193] Sequence was generated on an ABI Automatic sequencer using dyeterminator technology (U.S. Pat. No. 5,366,860; EP 272007) using acombination of vector and insert-specific primers. Sequence editing wasperformed in either DNAStar (DNA Star Inc.,) or the Wisconsin GCGprogram (Wisconsin Package Version 9.0, Genetics Computer Group (GCG),Madison, Wis.) and the CONSED package (version 7.0). All sequencesrepresent coverage at least two times in both directions.

[0194] Microarray of Gene Expression

[0195] Amplification of DNA regions for the construction of DNAmicroarray: Specific primer pairs were used to amplify each proteinspecifying ORF of Methylomonas sp. strain 16a. Genomic DNA (10-30 ng)was used as the template. The PCR reactions were performed in thepresence of HotStart Taq™ DNA polymerase (Qiagen, Valencia, Calif.) andthe dNTPs (Gibco BRL Life Science Technologies, Gaithersberg, Md.).Thirty-five cycles of denaturation at 95° C. for 30 sec, annealing at55° C. for 30 sec and polymerization at 72° C. for 2 min were conducted.The quality of PCR reactions was checked with electrophresis in a 1%argarose gel. The DNA samples were purified by the high-throughput PCRpurification kit from Qiagen.

[0196] Arraying amplified ORFs. Before arraying, an equal volume of DMSO(10 μL) and DNA (10 μL) sample was mixed in 384-well microtiter plates.A generation II DNA spotter (Molecular Dynamics, Sunnyvale, Calif.) wasused to array the samples onto coated glass slides (Telechem, Sunnyvale,Calif.). Each PCR product was arrayed in duplicate on each slide. Aftercross-linking by UV light, the slides were stored under vacuum in adesiccator at room temperature.

[0197] RNA isolation: Methylomonas 16a was cultured in a defined mediumwith ammonium or nitrate (10 mM) as nitrogen source under 25% methane inair. Samples of the minimal medium culture were harvested when the O.D.reaches 0.3 at A600 (exponential phase). Cell cultures were harvestedquickly and ruptured in RLT buffer [Qiagen RNeasy Mini Kit, Valencia,Calif.] with a beads-beater (Bio101, Vista, Calif.). Debris was pelletedby centrifugation for 3 min at 14,000×g at 4° C. RNA isolation wascompleted using the protocol supplied with this kit. After on-columnDNAase treatment, the RNA product was eluted with 50-100 μL RNAase-free.RNA preparations were stored frozen at either −20 or −80° C.

[0198] Synthesis of fluorescent cDNA from total RNA. RNA samples (7 to15 μg) and random hexamer primers (6 μg; Gibco BRL Life ScienceTechnologies) were diluted with RNAase-free water to a volume of 25 μL.The sample was denatured at 70° C. for 10 min and then chilled on icefor 30 seconds. After adding 14 μL of labeling mixture, the annealingwas accomplished by incubation at room temperature for 10 min. Thelabeling mixture contained 8 μL of 5× enzyme buffer, 4 μL DTT (0.1 M),and 2 μL of 20× dye mixture. The dye mixture consisted of 2 mM of eachdATP, dGTP, and dTTP, 1 mM dCTP, and 1 mM of Cy3-dCTP or Cy5-dCTP. Afteradding 1 to 1.5 μL of SuperScript II reverse transcriptase (200units/mL, Life Technologies Inc., Gaithersburg, Md.), cDNA synthesis wasallowed to proceed at 42° C. for 2 hr. The RNA was removed by adding 2μL NaOH (2.5 N) to the reaction. After 10 min of incubation at 37° C.,the pH was adjusted with 10 μL of HEPES (2M). The labeled cDNA was thenpurified with a PCR purification kit (Qiagen, Valencia, Calif.).Labeling efficiency was monitored using either A₅₅₀ for Cy3incorporation, or A650 for Cy5.

[0199] Fluorescent labeling of genomic DNA. Genomic DNA was nebulized toapproximately 2 kb pair fragments. Genomic DNA (0.5 to 1 μg) was mixedwith 6 μg of random hexamers primers (Gibco BRL Life ScienceTechnologies) in 15 μL of water. The mix was denatured by put at boilingwater for 5 minutes. Then anneal on ice for 30 sec before put at roomtemperature. Then 2 μL 5× Buffer 2 (Gibco BRL) and 2 ul dye mixture wereadded. The component of dye mixture and the labeling procedure are thesame as described above for RNA labeling, except that the Klenowfragment of DNA polymerase I (5 μg/μL, Gibco BRL Life ScienceTechnologies) was used as the enzyme. After incubation 37° C. for 2 hr,the labeled DNA probe was purified using a PCR purification kit (Qiagen,Valencia, Calif.).

[0200] Hybridization and washing Slides were first incubated withprehybridization solution containing 3.5×SSC (BRL, Life TechnologiesInc., Gaithersburg, Md.), 0.1% SDS (BRL, Life Technologies Inc.,Gaithersburg, Md.), 1% bovine serum albumin (BSA, Fraction V, Sigma, St.Louis, Mo.). After prehybridization, hybridization solutions (MolecularDynamics) containing labeled probes was added to slides and covered withcover slips. Slides were placed in a humidified chamber in a 42° C.incubator. After overnight hybridization, slides were initially washedfor 5 min at room temperature with a washing solution containing 1×SSC,0.1/O SDS and 0.1×SSC, 0.1% SDS. Slides were then washed at 65° C. for10 min with the same solution for three times. After washing, the slideswere dried with a stream of nitrogen gas.

[0201] Data Collection and Analysis. The signal generated from eachslide was quantified with a laser scanner (Molecular Dynamics,Sunnyvale, Calif.). The images were analyzed with ArrayVision 4.0software (Imaging Research, Inc., Ontario, Canada). The raw fluorescentintensity for each spot was adjusted by subtracting the background.These readings were exported to a spreadsheet for further analysis.

[0202] Table 1 is a description of the genes discovered and annotatedfor Methylomonas 16a. The table shows sequence % similarities, %identities, and expectation values for key genes of central carbonmetabolism, denitrification, exopolysacharride synthesis, and isoprenoidbiosynthesis.

[0203] Table 1 illustrates the relationship of these sequences to knownsequences in the art. All sequences were identified by conducting BLAST(Basic Local Alignment Search Tool; Altschul, S. F., et al., (1993) J.Mol. Biol. 215:403-410; see also www.ncbi.nlm.nih.gov/BLAST/) searchesfor similarity to sequences contained in the BLAST “nr” database(comprising all non-redundant GenBank CDS translations, sequencesderived from the 3-dimensional structure Brookhaven Protein Data Bank,the SWISS-PROT protein sequence database, EMBL, and DDBJ databases). Thesequences were analyzed for similarity to all publicly available DNAsequences contained in the “nr” database using the BLASTN algorithmprovided by the National Center for Biotechnology Information (NCBI).The DNA sequences were translated in all reading frames and compared forsimilarity to all publicly available protein sequences contained in the“nr” database using the BLASTX algorithm (Gish, W. and States, D. J.(1993) Nature Genetics 3:266-272) provided by the NCBI. All comparisonswere done using either the BLASTNnr or BLASTXnr algorithm. The resultsof the BLAST comparison is given below in Table 1 which summarize thesequences to which they have the most similarity. Table 1 displays databased on the BLASTXnr algorithm with values reported in expect values.The Expect value estimates the statistical significance of the match,specifying the number of matches, with a given score, that are expectedin a search of a database of this size absolutely by chance. TABLE 1Genes Characterized From Methylomonas 16a Similarity SEQ ID % % GeneName Identified SEQ ID Peptide Identity^(a) Similarity^(b) E-value^(c)Citation Phosphogluco- Phosphogluco- 1 2 65% 85% 1.7e-140 Lepek et al.,Direct mutase mutase Submission (Glucose |gb|AAD03475.1| Phosphomutase)(Pgm)>>gi|32 41933|gb|AA D03475.1| Glucose 6 Glucose 6 3 4 64% 81%1.6e-136 Blattner et al., Nucleic phosphate phosphate Acids Res. 21(23), isomerase isomerase 5408-5417 (1993) gi|396360|gb| AAC43119.1Phosphofructo Phosphofructo- 5 6 63% 83% 1.7e-97 Ladror et al., J. Biol.kinase kinase Chem. 266,16550- 16555 (1991) pyrophosphate pyrophosphatedependent dependent gi|150931|gb| AAA25675.1| (M67447) 6- 6- 7 8 60% 85%1.6e-141 Willis et al., J. Bacteriol. Phosphogluconate Phosphogluconate181 (14), 4176-4184 dehydratase dehydratase (1999) gi|4210902|gb|AAD12045.1| (AF045609) Glucose 6 Glucose 6 9 10 58% 85% 9.4e-123Hugouvieux-Cotte- phosphate 1 phosphate 1 Pattat, N, TITLE Directdehydrogenase dehydrogenase Submission, gi|397854|embgi|3978541|emb|CAA528 |CAA52858.1| 58.1|(X74866) (X74866) TALTransaldolase 11 12 78% 90% 2.7e-92 Plant Mol. Biol. 30 (1), 213-218(1996) MIPB Transaldolase 13 14 50% 79%   1e-23 Blattner F.R. et. alScience 277:1453- 1474 (1997). FBA or FDA Fructose 15 16 76% 92%4.1e-111 Alefounder P.R. et. al. bisphosphate Mol. Microbiol. 3:723-aldolase 732 (1989). FBA or FDA Fructose 17 18 40% 70% 2.3e-39 van denBergh E.R. bisphosphate et al.; aldolase J. Bacteriol. 178:888- 893(1996) KHG/KDPG (AL352972) 19 20 59% 72%   1e-64 Redenbach et al., Mol.KHG/KDPG Microbiol. 21(1), 77- aldolase 96 (1996) Streptomycescoelicolor ugp ugp 21 22 58% 82% 3.2e-60 Wei et al., Biochem.(Xanthomonas Biophys. Res. campestris) Commun. 226 (3), 607- 612 (1996)gumD gumD 23 24 36% 69% 2.5e-52 Chou,F.L., et el, (Xanthomonas Biochem.Biophys. campestris) Res. Commun. 233 (1), 265-269 (1997) wza wza 25 2636% 69% 5.8e-39 Blattner,F.R.et al., (Escherichia Science 277 (5331),coli) 1453-1474 (1997) epsB epsB 27 28 35% 67%   2e-74 Huang,J. and(Pseudomonas Schell, M., Mol. solanacearum) Microbial. 16 (5), 977- 989(1995) epsM epsM 30 20 23% 55% 1.3e-05 Stingele,F. et al.,,(Streptococcus J. Bacterial. 178 (6), thermophilus) 1680-1690 (1996)waaE waaE 31 32 28% 55% 8.6e-09 Pique,N et al.,. (Serratia Unpublishedmarcescens) Genbank number: AAC44433 epsV epsV 33 34 21% 56% 2.3e-05Bourgoin,F. et al., (Streptacoccus Plasmid 40 (1), 44-49 thermophilus)(1998) gumH gumH 35 36 26% 55% 0.00088 Becker,A. et al., Mol. (RhizobiumMicrobial. 16 (2), 191-203 meliloti) (1995) glycosyl Glycosyltrans 37 3851% 80% 1.7e-62 Nakano,Y, Biochem. transferase ferase Biophys. Acta(Actinobacillus 1442:409-414 (1998) actinimycetemcomitans) nirF NirFprotein 39 40 59% 85% 1.3e-92 Palmedo et al., Eur. J. (Pseudomonas)Biochem. 232 (3), 737- 746 (1995) nirD NirD protein 41 42 49% 76%1.7e-22 Palmedo et al., Eur. J. (Pseudomonas) Biochem. 232 (3), 737- 746(1995) nirL NirL protein 43 44 49% 73% 6.4e-28 Palmedo et al., Eur. J.(Pseudomonas) Biochem. 232 (3), 737- 746 (1995) nirG NirG protein 45 4649% 80% 1.6e-25 Kawasaki et al., J. (Pseudomonas) Bacteriol. 179 (1),235- 242 (1997) nirH NirH protein 47 48 59% 78% 9.9e-33 Kawasaki et al.,J. (Pseudomonas) Bacteriol. 179 (1), 235- 242 (1997) nirJ NirJ protein49 50 56% 81% 5.1e-88 Kawasaki et al., J. (Pseudomonas) Bacteriol. 179(1), 235- 242 (1997) nasA Nitrate 51 52 51% 74% 9.2e-123 LIN J.T.,GOLDMAN reductase B.S., STEWART V.; J. Kiebsiella Bacteriol.175:2370-2378 (1993). norC Nitric-oxide 53 54 32% 70%   1e-08 Zumft etal., Eur. J. reductase Biochem. 219:481-490 subunit C (1994).(Pseudomonas) norB Nitric-oxide 55 56 39% 70% 3.5e-64 Zumft et al., Eur.J. reductase Biochem. 219:481-490 subunit B (1994). (Pseudomonas) norZCytochrome 57 58 39% 69% 1.7e-100 Cramm,R., B Siddiqui,R.A. and subunitof Friedrich,B. J. nitric oxide Bacteriol. 179 reductase (21), 6769-6777(Alcaligenes) (1997). norS Nitrite 59 60 28% 59% 2.1e-25 Glockner,A.B.and reductase Zumft,W.G. Biochim. (cytochrome Biophys. Acta 1277 cd1)(1-2), 6-12 (1996) (Pseudomonas) dxs 1- 61 62 60% 86% 5.7e-149Lois,L.M., et al., Proc. deoxyxylulos Natl. Acad. Sci. U.S.A. e-5- 95(5), 2105-2110 phosphate (1998) synthase dxr 1-deoxy- 63 64 55% 78%3.3e-74 Takahashi S et al., dxylulose 5- Proc. Nati. Acad. Sci.phosphate U.S.A. 95:9879- reductoisome 9884 (1998). rase ygbB/ispF2C-methyl-d- 65 66 69% 84% 1.6e-36 Herz 5, et al., erythritol 2,4- ProcNatl Acad Sci U S a cyclodiphosp 2000 Mar hate synthase 14;97(6):2486-90 ygbP/ispD 2C-methyl-d- 67 68 52% 74% 7.7e-36 Rohdich F, etal., erythritol Proc Natl Acad Sci U S cytidylyltransf A 1999 Oct erase12;96 (21 ): 11758-63 pyrG CTP 69 70 67% 89% 2.4e-141 Weng M., J. etal., Biol. synthase Chem. 261:5568- 5574(1986). IspA Geranyltranst 71 7256% 78% 7.8e-56 Ohto,C et al., ransferase Plant Mol. Biol. 40 (2), (also307-321 (1999) farnesyldiphosphate synthase) ychB/IspE 4- 73 74 50% 72%8.8e-49 Luttgen H, diphosphocytidyl- Proc Natl Acad Sci U S 2-C- A. 2000Feb methylerythritol 1;97 (3): 1062-7. kinase crtN1 diapophytoene 75 7634% 72% 4e-66 Xiong,J dehydrogenase Proc. Natl. Acad. Sci. CrtN- U.S.A.95 (25), copy 1 14851-14856 (1998) crtN2 Diapophytoene 77 78 49% 78%1.3e-76 Wieland,K.P. and dehydrogena Goetz,F. Unpublished se CrtNù copy2 Particulate probable 79 80 71% 85% 0.0 Semrau et al., J. methanemethane Bacteriol. 177 (11), monooxygenase monooxygenase 3071-3079(1995) 45k chain- Methylococcus capsulatus B57266 GI:2120829

Example 1 Isolation of Methylomonas 16a

[0204] The original environmental sample containing the isolate wasobtained from pond sediment. The pond sediment was inoculated directlyinto defined medium with ammonium as nitrogen source under 25% methanein air. Methane was the sole source of carbon and energy. Growth wasfollowed until the optical density at 660 nm was stable whereupon theculture was transferred to fresh medium such that a 1:100 dilution wasachieved. After 3 successive transfers with methane as sole carbon andenergy source the culture was plated onto growth agar with ammonium asnitrogen source and incubated under 25% methane in air. Manymethanotrophic bacterial species were isolated in this manner. However,Methylomonas 16a was selected as the organism to study due to the rapidgrowth of colonies, large colony size, ability to grow on minimal media,and pink pigmentation indicative of an active biosynthetic pathway forcarotenoids.

Example 2 Rapid Growth on Methane in Minimal Medium

[0205] Methylomonas 16a grows on the defined medium comprised of onlyminimal salts, a culture headspace comprised of methane in air. Methaneconcentrations for growth but typically are 5-50% by volume of theculture headspace. No organic additions such as yeast extract orvitamins are required to achieve growth shown in FIG. 1. FIG. 1 showsthe growth of 16a compared to the growth of Methylococcus capsulatusunder identical growth conditions. i.e. minimal medium with 25% methanein air as substrate. The data indicates Methylomonas 16a doubles every2-2.5 h whereas Methylococcus capsulatus doubles every 3.5 h withmethane as substrate. With methanol as substrate doubling times onmethanol are 2.5-3 for Methylomonas 16a and 4.5-5 for Methylococcuscapsulatus. Cell densities are also significantly higher forMethylomonas 16a growing on methane. Methylococcus capsulatus is awidely utilized methanotroph for experimental and commercial purposes.

Example 3 Methanol Tolerance

[0206] Methylomonas 16a was grown on defined medium with nitrate as solenitrogen source and methanol as sole carbon source. Growth was monitoredover a 36 hr period which was typically sufficient for attaining maximumoptical density or turbidity of the culture. FIG. 2 clearly shows thatmaximum growth or turbidity is attained within 36 hours at methanolconcentrations up to 600 mM. However no growth was observed at 800 mM.Therefore the strain is shown to grow on 2.4% (vol/vol) of methanol.

Example 4 Properties and Classification of Methylomonas 16a

[0207] Table 2 shows the various properties of Methylomonas 16a. Thecriteria listed in Table 2 are those typically used to determine whetherthe strain is arbitrarily considered Type I, Type II or Type X based onphysical and enzymatic properties. This table was developed from bothdirect enzymatic assay for enzymes as well as genomic data showing thepresence of genes and gene pathways. This categorization is functionallybased and indicates that the strain utilizes the most energeticallyefficient pathway for carbon incorporation which is the ribulosemonophosphate or “RuMP” pathway. Genomic data clearly shows the presenceof key enzymes in the RuMP pathway. Internal membrane structure are alsoindicative of a Type I physiology. Unique to the present strain is thefinding of nitrogen fixation genes in Methylomonas 16a. The strain isshown to grow in the absence of yeast extract or vitamins. Nitrate,ammonium ion or dinitrogen can satisfy the nitrogen requirement forbiosynthesis. This functional data is in complete agreement with the16srRNA homologies as compared with other Methylomonas strains. 16sRNAcomparisons or the 16a strain (SEQ ID NO:81) with other Methylomonas sp.revealed that Methylomonas 16a has 96% identity with the 16sRNA ofMethylomonas sp. (strain:KSPIII) [Hanada, S et al., J. Ferment. Bioeng.86, 539-544 (1998)] and with Methylomonas sp. (strain LW13), [Costello,A. M. and Lidstrom, M. E. Appl. Environ. Microbiol. 65 (11), 5066-5074(1999)]]. Thus Methylomonas 16a is correctly classified as a Type I,RuMP utilizing, Methylomonas species. TABLE 2 MethylomonasCharacteristic Type I 16a Type X Type II % GC Incomplete IncompleteIncomplete Complete Ribmp Cycle Incomplete Incomplete IncompleteComplete RuBP − − + + Carboxylase Temp. Range <45 <42 <45 <40Nitrogenase − + + + G6P + + + − dehydrogenase NADP Isocitrate + + − −dehydrogenase NAD/NADP Yeast Extract − − − − Vitamins − − − −Pigmentation Variable + Variable Variable Nitrate + + + + assimilation

[0208] Method of Enzymatic Assay

[0209] Nitrogenase was not assayed but is considered positive if thegene is present on the basis of genome sequence analysis.

[0210] Glucose 6 phosphate dehydrogenase: One mL of reaction mixturecontains 100 μL of 10 mM NADP, 100 μL of 10 mM glucose, 700 μL of 100 mMHEPES pH 7 buffer and up to 100 μL of enzyme extract. The enzymeactivity was measured by monitoring NADP reduction to NADPH at 340 nmusing spectrophotometer.

[0211] Isocitrate dehydrogenase: One mL of reaction mixture contains 100μL of 10 mM sodium isocitrate, 100 μL of 10 mM NADP, 700 μL of 100 mM pH7 HEPES buffer up to 100 μL of enzyme extract. The enzyme activity wasmeasured by monitoring NADPH formation at 340 nm.

[0212] Nitrate assimilation is based on the ability of the strain togrow on nitrate as sole nitrogen source.

[0213] The results of the enzyme assay are sown in Table 2.

Example 5 Comparison of Gene Expression Levels in the Entner DouderoffPathway as Compared with the Embeden Meyerhof Pathway

[0214] Example 5 presents microarray evidence for the use of theEmbden-Meyerhof pathway in the 16a strain.

[0215]FIG. 3 shows the relative levels of expression of genes for theEntner-Douderoff pathway and the Embden-Meyerhof pathway. The relativetranscriptional activity of each gene was estimated with DNA microarrayas described previously (Wei, et al., 2001. Journal of Bacteriology.183:545-556).

[0216] Specifically, a single DNA microarray containing 4000 ORFs (openreading frames) of Methylomonas sp. strain 16a was hybridized withprobes generated from genomic DNA and total RNA. The genomic DNA of 16awas labeled with Klenow fragment of DNA polymerase and fluorescent dyeCy-5, while the total RNA was labeled with reverse transcriptase andCy-3. After hybridization, the signal intensities of both Cy-3 and Cy-5for each spot in the array were quantified. The intensity ratio of Cy-3and Cy-5 was then used to calculate the fraction of each transcript (inpercentage) with the following formula: (gene ratio/sum of allratio)×100. The value obtained reflects the relative abundance of mRNAof an individual gene. Accordingly, transcriptional activity of all thegenes represented by the array can be ranked based on its relative mRNAabundance in a descending order. For example, mRNA abundance for themethane monooxygenase was ranked #1 because its genes had the highesttranscriptional activity when the organism was grown with methane as thecarbon source (FIG. 3).

[0217] The genes considered “diagnostic” for Entner-Douderoff are the 6phosphogluconate dehydratase and the 2 keto-3-deoxy-6-phosphogluconatealdolase. Phosphofructokinase and fructose bisphosphate aldolase are“diagnostic” of the Embden-Meyerhof sequence. Numbers in FIG. 3 next toeach step indicate the relative expression level of that enzyme. Forexample the most highly expressed enzyme in the cell is the methanemonooxygenase (ranked #1). The next most highly expressed is themethanol dehydrogenase (ranked #2). Messenger RNA transcripts ofPhosphofructokinase (ranked #232) and fructose bisphosphate aldolase(ranked #65) were in higher abundance than those for glucose 6 phosphatedehydrogenase (ranked #717), 6 phosphogluconate dehydratase (ranked#763) or the 2-keto-3-deoxy-6-gluconate aldolase. The data suggests thatthe Embden-Meyerhof pathway enzymes are more strongly expressed than theEntner-Douderoff pathway enzymes. This result is surprising and counterto existing beliefs on the central metabolism of methanotrophic bacteria(Reference book pages in. The physiology and biochemistry of aerobicmethanol-utilizing gram-negative and gram-positive bacteria In: Methaneand Methanol Utilizers, Biotechnology Handbooks 5. 1992. Eds: ColinMurrell, Howard Dalton. Pp 149-157.

Example 6 Direct Enzymatic Evidence for a Pyrophosphate-LinkedPhosphofructokinase

[0218] Example 6 shows the evidence for the presence of apyrophosphate-linked phosphofructokinase enzyme in the current strainwhich would confirm the functionality of the Embden-Meyerhof pathway inthe present strain.

[0219] Phosphofructokinase activity was shown to be present inMethylomonas 16a by using the coupled enzyme assay described below.Assay conditions are given in Table 3 below. This assay was further usedto assay the activity in a number of other Methanotrophic bacteria asshown below in Table 4. The data in Table 4 show known ATCC strainstested for phosphofructokinase activity with ATP or pyrophosphate asphosphoryl donor. These organisms were classified as either Type I orType X ribulose monophosphate-utilizing strains or Type II serineutilizer.

[0220] Coupled Assay Reactions

[0221] Phosphofructokinase reaction is measured by a coupled enzymeassay. Phosphofructokinase reaction is coupled with fructose 1,6,biphosphate aldolase followed by triosephosphate isomerase. The enzymeactivity is measured by the disappearance of NADH.

[0222] Specifically, the enzyme phosphofructokinase catalyzes the keyreaction converting Fructose 6 phosphate and pyrophosphate to Fructose1,6 bisphosphate and orthophosphate.

[0223] Fructose-1,6-bisphosphate is cleaved to 3-phosphoglyceraldehydeand dihydroxyacetonephosphate by fructose 1,6-bisphosphate aldolase.

[0224] Dihydroxyacetonephosphate is isomerized to3-phosphoglyceraldehyde by triosephosphate isomerase.

[0225] Glycerol phosphate dehydrogenase plus NADH and3-phosphoglyceraldehyde yields the alcohol glycerol-3-phosphate and NAD.Disappearance of NADH is monitored at 340 nm using spectrophotometer(UltraSpec 4000, Pharmacia Biotech). TABLE 3 Assay Protocol Volume (μl)per Final assay Stock solution 1 mL total concentration Reagent (mM)reaction volume (mM) Tris-HCl pH 7.5 1000 100 100 MgCl₂.2 H₂O 100 35 3.5Na₄P₂O₇.10H₂O 100 20 2 or ATP Fructose-6- 100 20 2 phophate NADH 50 60.3 Fructose 100 (units/mL) 20 2 (units) bisphosphate aldolase Triosephosphate (7.2 units/μl) 3.69  27 units isomerase/glycerol (0.5units/μl) 1.8 units phosphate dehydrogenase KCl 1000 50 50 H2O adjust to1 mL Crude extract 0-50

[0226] TABLE 4 Comparison Of Pyrophosphate Linked And ATP LinkedPhosphofructokinase Activity In Different Methanotrophic BacteriaATP-PFK umol Ppi-PFK Assimilation NADH/ umol NADH/ Strain Type Pathwaymin/mg min/mg Methylomonas 16a I Ribulose 0 2.8 ATCC PTA 2402monophosphate Methylomonas I Ribulose 0.01 3.5 agile monophosphate ATCC35068 Methylobacter I Ribulose 0.01 0.025 Whittenbury monophosphate ATCC51738 Methylomonas I Ribulose 0 0.3 clara monophosphate ATCC 31226Methylomicrobium I Ribulose 0.02 3.6 albus monophosphate ATCC 33003Methylococcus X Ribulose 0.01 0.04 capsulatus monophosphate ATCC 19069Methylosinus II Serine 0.07 0.4 sporium ATCC 35069

[0227] Several conclusions may be drawn from the data presented above.First, it is clear that ATP (which is the typical phosphoryl donor forphosphofructokinase) is essentially ineffective in thephosphofructokinase reaction in methanotrophic bacteria. Only inorganicpyrophosphate was found to support the reaction in all methanotrophstested. Secondly not all methanotrophs contain this activity. Theactivity was essentially absent in Methylobacter whittenbury and inMethylococcus capsulatus. Intermediate levels of activity were found inMethylomonas clara and Methylosinus sporium. These data show that manymethanotrophic bacteria may contain a hitherto unreportedphosphofructokinase activity. It may be inferred from this thatmethanotrophs containing this activity have an active Embden-Meyerhofpathway.

Example 7 Growth Yield and Carbon Conversion by Methylomonas 16a

[0228] Growth yield and carbon conversion efficiency were compared forMethylomonas 16a and Methylococcus capsulatus. These strains were chosenbecause 16a contains high levels of phosphofructokinase and M.capsulatus is essentially devoid of the enzyme activity. It wascontemplated that if Methylomonas 16a could utilize the moreenergetically favorable Embden-Meyerhof pathway and Methylococcuscapsulatus could only use the Entner-Douderoff pathway the superiorenergetics of the present Methylomonas 16a strain would be reflected incellular yields and carbon conversion efficiency. This difference inenergetic efficiency would only be apparent under energy-limitingconditions. These conditions were achieved in this experiment bylimiting the amount of oxygen in each culture to only 10% (vol/vol)instead of 20% (growth conditions employed in FIG. 1 and Table 9). Underthese oxygen limiting conditions the strain that produces the mostenergy from aerobic respiration on methane will produce more cell mass.

[0229] Cells were grown as 200 mL cultures 500 mL serum-stopperedWheaton bottles. The headspace in the bottles was adjusted to 25%methane and 10% oxygen. The defined medium formulation is the same inboth cases. TABLE 5 Yield Of Methylomonas 16a Cells Versus MethylococcusCapsulatus Cells Under Oxygen Limitation. Carbon Conversion EfficiencyStrain Y_(CH4 g dry wt/mol) G dry wt/g CH₄ (CCE) % Methylomonas 16.7 +/−0.5 1.04 64 16a Methylococcus 10.3 +/− 0.3 0.64 40 capsulatus

[0230] Yield determination: Yield was measured by growing triplicatecultures in 500 mL bottles on defined medium with ammonium as nitrogensource under oxygen limitation. This was done by using 300 mL of culturewith a 300 mL headspace of 25% methane and 10% oxygen the balance beingnitrogen. At the end of growth (i.e. stationary phase) residual methanein the headspace was determined by gas chromatography. The cells werecollected by centrifugation washed with distilled water and driedovernight in a drying oven before being weighed.

[0231] Carbon conversion efficiency is a measure of how much carbon isassimilated into cell mass. It is calculated assuming a biomasscomposition of CH₂O_(0.5)N_(0.25):

[0232] Methylomonas 16a: 16 g/mol methane×(1 g dry wt/g methane)/25g/mol biomass

[0233]M. capsulatus 16 g/mol methane×(0.64 g dry wt/g methane)/25 g/molbiomass

[0234] These data (in Table 5) show that Methylomonas 16a producedsignificantly more cell mass than did the Methylococcus capsulatusstrain under growth conditions that were identical except for thetemperature. Methylococcus capsulatus grows optimally at 45° C. whereasMethylomonas is grown at 33° C. It may be inferred from the data thatthe presence of the more energy-yielding Embden-Meyerhof pathway confersa growth advantage to Methylomonas 16a.

[0235] Table 6 presents the theoretical calculations showing ATP yieldas a function of carbon assimilation pathway with the carbon outputbeing normalized to pyruvate in all cases (The physiology andbiochemistry of aerobic methanol-utilizing gram-negative andgram-positive bacteria In: Methane and Methanol Utilizers, BiotechnologyHandbooks 5.1992. Eds: Colin Murrell, Howard Dalton. Pp. 149-157). Table6 shows the amount of ATP that is produced or consumed for every threemolecules of carbon (as formaldehyde or carbon dioxide) for serinecycle, xylulose monophosphate cycle and ribulose monophosphate cyclepathways. The latter pathway, as discussed is typically thought to existas the 2-keto-3deoxy-6-phosphogluconate/transaldolase (KDPGA/TA)variant. These data shows that in fact the fructose bisphosphatealdolase/transaldolase (FBPA/TA) variant is likely to exist in themethanotrophs. The energetic repercussion of this is the net productionof an additional 1 ATP for methanotrophs if they possess an ATP linkedphosphofructokinase and an additional 2 ATPs for thepyrophosphate-linked enzyme. It is therefore expected that Methylomonas16a derives and additional 2 ATP per 3 carbons assimilated and that thismay explain the greater yield and carbon efficiency of the strain versusMethylococcus capsulatus. TABLE 6 Energetics of Methanotrophic bacteriautilizing different carbon assimilation mechanisms C1 unit OrganismCycle fixed Product Variant ATP NADPH Bacteria RuMP 3CH₂O PyruvateFBPA/TA +1 +1 Methylomonas RuMP/Serine 3CH₂O Pyruvate FBPA/TA +1(+2*) +1Bacteria RuMP 3CH₂O Pyruvate KDPGA/TA   0 +1 Methylococcus RuMP/RuBP3CH₂O Pyruvate KDPGA/TA   0 +1

Example 8 Nitrate/Nitrite Spares Oxygen

[0236]FIG. 4 shows oxygen uptake by a cell suspension of Methylomonas16a, in relative detector units, using an Orion oxygen probe (Orion, UK)to detect oxygen consumption. Oxygen was measured as a function of timein the presence or absence of nitrate and in the presence of methanol aselectron donor and carbon source. The incubation consisted ofMethylomonas 16a cells suspended in HEPES buffer pH 7. Methanol wasinjected at 3 min into both incubations to achieve a final concentrationof about 100 mM. After the methanol injection it can be seen that oxygenuptake accelerated as would be expected (FIG. 4) in the cultures withoutnitrate. However the rate of oxygen uptake in the presence of nitratenever approaches that of cells without nitrate. The data thus supportsthe finding that nitrate can spare oxygen consumption with methanol ascarbon source.

[0237] Methylomonas 16a cells were again suspended in HEPES buffer pH 7and incubated in a water jacketed chamber equipped with an Orion oxygenprobe. The incubation was carried out at 300C. Methanol was injectedinto the incubation at 1 min. However in one incubation sodium nitrite(25 mM) was injected into the incubation after 23 min. The results areshown in FIG. 5. As seen in FIG. 5, there is a decrease in the rate ofoxygen uptake after the addition of nitrite. This data again clearlysupports the assertion that nitrite and indirectly nitrate can be usedas an alternative electron sink and resulting in less oxygen consumptionby the culture.

[0238] A cell suspension of Methylomonas 16a in defined medium under 25%methane in air was simultaneously monitored for oxygen and N₂O in thedead-space. 100 mM Nitrite was the only added source of nitrogen. Theresults are shown in FIG. 6. FIG. 6 illustrates that the appearance ofN₂O in the dead-space coincides with oxygen depletion. The numbersplotted are the rates of appearance or disappearance of N₂O and oxygenrespectively. As oxygen disappearance rates decline to lower values (dueto lower headspace O₂ concentrations) N₂O production increases to becomea significant fraction of the total electron flow through the organism(only under oxygen limitation).

Example 9 Nitrate or Nitrite Reduction by Other Strains of Methanotrophsand Methylomonas 16a

[0239] All methanotrophic strains available from the American TypeCulture collection were tested for their ability to produce N₂O fromnitrite or nitrate. All strains were grown on the defined medium andharvested after an optical density at 660 nm of 1.0 was achieved. Thecell suspensions were collected by centrifugation and resuspended in 5mL of defined medium with either nitrate or nitrite as sole nitrogensource. The data in Table 7 below shows the accumulation of N₂O (in uMconcentration) in the headspace of a 10 mL assay vial incubated 300C.The results shows that Methylomonas 16a has a unique ability to convertnitrate to N₂O among the strains tested. Furthermore the data show thattwo other Methylomonas strains have a similar ability to convert nitriteto N₂O. TABLE 7 NO₃/NO₂ NO₂/N₂O STRAIN uM uM Methylomonas 16a 28.3 30Methylomonas albus 1.2 22 Methylomonas clara 2.5 1.5 Methylomonas agile0.6 17 Methylobacter whitterbury 0.3 0.04 Methylococcus capsulatis 0.31.9 Methylobacter lutes 0.1 6.5 Methylosinus sporium 0.2 0.07

Example 10 Production of Glycogen

[0240] Methylomonas 16a was shown to accumulate large amounts ofglycogen when grown on either methane or methanol. Methylomonas cellswere analyzed for glycogen using a starch assay kit (Sigma Chemical Co.St Louis Mo.). This assay is starch or glycogen specific andconclusively shows the presence of glycogen in Methylomonas 16a. Cellswere grown according to the conditions outlined in the General Methods,Cells were harvested during growth on 100 mM methanol or 25% headspacemethane at 30° C. on defined medium. Culture samples were taken at twopoints in the growth curve: mid-logarithmic growth (O.D. 660 0.3) andstationary phase (O.D. 660 1.0). These samples were immediately analyzedwith the starch assay kit according to the manufacturers instructions.The results shown below in Table 8 indicate surprising amounts of thestorage polymer during growth on methanol and lower but significantamounts of glycogen during growth on methane. TABLE 8 Methane (%glycogen Methanol (% glycogen Growth Phase (OD660) wt/wt) (wt/wt)Mid-log (0.3) 6% 25% Stationary phase (1.0) 7% 40%

[0241] Additionally, the presence of granules within the cells grown onmethanol were observed by scanning electron microscopy and the granuleswere determined to contain starch with polysaccharide specific stains.

Example 11 Production of Protein from Cell Mass

[0242] Methylomonas 16a and Methylococcus capsulatus (reference strainfor protein production) were grown on defined medium until no furtherincreases in OD 660 could be observed. Methane or methanol consumptionwas monitored by gas chromatography (HP-Plot Molecular sieve column;Hewlett Packard 5890 series II gas chromatograph) over the growth curvesuch that the total amount of methane or methanol consumed could becalculated. The running conditions for GC were; oven temperature: 40°C., initial temperature: 40° C., initial time: 3 min, rate: 0 deg/min,final temperature 40° C., final time 0, injection A temperature: 100°C., Det. A temperature: 125° C., and equilibration time: 0.

[0243] The cells were collected by centrifugation and dried overnight ina 105° C. drying oven. The data in Table 9 below shows the gram dryweight of cells produced per gram of methane or methanol consumed. TABLE9 Organism g dry wt./g CH₄ g dry wt./g CH₄OH Methylomonas 16a 0.90-1.3(2-2.5 hr) 0.30-0.45 (2.5-3.0 hr) Methylococcus 0.67-1.2 (3-4 hr)0.25-0.45 (4-5 hr) capsulatus

[0244] As can be seen by the data in Table 9 the present strain has ahigher rate of protein production than the commercial methanotroph ofchoice for this process, when grown on either methane or methanol.

Example 12 Production of Extracellular Polysacharride

[0245] Methylomonas 16a cells were grown on 25% methane in 200 mL batchculture on defined medium at 300C. Initial oxygen concentration wasvaried by injecting pure oxygen into nitrogen flushed bottles. Cellswere allowed to grow until stationary phase or to an optical density ofapproximately 1.0. At that time the cultures were centrifuged at 6000×gfor 30 min to sediment both the cells and the extracellularpolysaccharide. The sediments from these centrifugations comprised twolayers. At the bottom were the cells, overlaid with a clear viscousmaterial which was the extracellular polysaccharide (EPS). The EPS layerwas washed off and pelleted again for further separation from the cells.The cell pellet was also dried and weighed. The EPS was resuspended in50% ethanol and pelleted again in the centrifuge. Finally the materialwas dried and weighed. EPS was found to comprise as much as 50% of thetotal dry weight of the culture at near-ambient oxygen concentrations.This was determined by centrifugation of the culture at 10,000×g for 30min. The resulting pellet is comprised of a lower red phase (packedcells) and an upper translucent phase which is the extracellularpolysaccharide. The EPS was selectively removed with a spatula and driedat 105° C. overnight. The cell pellet was removed and dried at 105° C.overnight. The supernatant from the centrifugation was mixed with coldisopropanol (1:1 vol:vol). The precipitated EPS from this step wascollected by centrifugation (10,000×g for 30 min) and the pellet driedat 105° C. overnight and weighed. Chemical analysis of the EPS revealedthat it was primarily polyglucose (−70%). EPS samples were methylated bythe method of Ciucanu, I., F. Kerek. 1984. Carbohydrate Research131:209-217. The methylated samples were hydrolyzed in 2 M TFA at 121°C. for 2 hours and the hydrolyzed carbohydrate was reduced with sodiumborodeuteride at room temperature. The product was acetylated by GC-MSusing Sp2330 Supelco column. Internal standard myo-inositol was added toeach sample prior to the reduction step.

1 81 1 1434 DNA METHYLOMONAS SP. 1 aacatgcaaa taaaaaccta taagaccacaccctatgatg atcaaaaacc cggcacatcc 60 gggctaagaa aaaaggttaa agtttttcagcaatccggct atctggaaaa tttcgttcag 120 tccattttca atagtttaga agattttcagggaaaaattc tagttttagg cggcgacggc 180 cgatatttta atcgacaagc gattcagatcatcatcaaaa tggcggccgc taacgggttt 240 ggtgagctga tcatcggcca gggcggtctgttgtcgacac cggcggcctc caatgtcatc 300 cgcaaatatc gcgctttcgg cggcatcattctatccgcca gccacaatcc cggtggtccc 360 gacgaagact tcggcatcaa atataacgtcggcaatggcg ggccggcacc ggaaaagttc 420 accgacgcct tgttcgaaaa cagcaaaaccatcaccagct atcagatggc cgaaatcgac 480 gacatcgatc tcgatagcgt cggcgacgtccaaatcgatg gcataacaat ccgcatcatc 540 gatcccgtgg ccgattacgc cgaattgatggcccggattt tcgatttcga cctgatcaag 600 caaagcatcg ccgccggctt gattaccttgcgcttcgacg cgatgcatgc cattaccggc 660 ccctatgcca aacatatact cgaagacgtgctgggcgccg cgcccggttc ggtattcaac 720 gccgtaccgc tggaagactt cggcggcggccatcccgatc ccaacatggc gcacgcgcac 780 gagctcaccg aaatcatgtt cgggccgaatccgccggttt tcggcgcggc ctcggacggt 840 gacggcgacc gcaacatgat catgggcgccaatattttcg tcacccccag cgacagtctg 900 gccatcatgg cggccaacgc gcaattgattcccgcctatg ccaagggcat tagcggcgtc 960 gcccgctcga tgccgaccag ccaggcggtcgacagggtcg cggataaatt gagtctgccg 1020 tgctacgaaa cgccgaccgg ctggaaattctttggcaatt tgctggatgc cgacaaaatc 1080 acgctgtgcg gcgaagaaag cttcggttccggttccaatc atgtccggga aaaagacggc 1140 ttgtgggccg ttttattttg gctgaatttgcttgcgcgca agcgtcaacc ggccgaggat 1200 atcgtgcgtg aacattggca aaaatacggccgcgacatct attgccgcca tgattacgaa 1260 gccgtcgatg ccgacatcgc caacggcatcgtagagcagc tgcgaaacca attgccgagc 1320 ttgcccggca aaacctgggg cgattacagcgtcaaattcg ccgacgaatt cagctatacc 1380 gatccggtcg atggtagcgt cagcagcaaccaaggcatcc gcgtcggttt cgcc 1434 2 545 PRT METHYLOMONAS SP. 2 Asn Met GlnIle Lys Thr Tyr Lys Thr Thr Pro Tyr Asp Asp Gln Lys 1 5 10 15 Pro GlyThr Ser Gly Leu Arg Lys Lys Val Lys Val Phe Gln Gln Ser 20 25 30 Gly TyrLeu Glu Asn Phe Val Gln Ser Ile Phe Asn Ser Leu Glu Asp 35 40 45 Phe GlnGly Lys Ile Leu Val Leu Gly Gly Asp Gly Arg Tyr Phe Asn 50 55 60 Arg GlnAla Ile Gln Ile Ile Ile Lys Met ala Ala Ala Asn Gly Phe 65 70 75 80 GlyGlu Leu Ile Ile Gly Gln Gly Gly Leu Leu Ser Thr Pro Ala Ala 85 90 95 SerAsn Val Ile Arg Lys Tyr Arg Ala Phe Gly Gly Ile Ile Leu Ser 100 105 110Ala Ser His Asn Pro Gly Gly Pro Asp Glu Asp Phe Gly Ile Lys Tyr 115 120125 Asn Val Gly Asn Gly Gly Pro Ala Pro Glu Lys Phe Thr Asp Ala Leu 130135 140 Phe Glu Asn Ser Lys Thr Ile Thr Ser Tyr Gln Met ala Glu Ile Asp145 150 155 160 Asp Ile Asp Leu Asp Ser Val Gly Asp Val Gln Ile Asp GlyIle Thr 165 170 175 Ile Arg Ile Ile Asp Pro Val Ala Asp Tyr Ala Glu LeuMet ala Arg 180 185 190 Ile Phe Asp Phe Asp Leu Ile Lys Gln Ser Ile AlaAla Gly Leu Ile 195 200 205 Thr Leu Arg Phe Asp Ala Met His Ala Ile ThrGly Pro Tyr Ala Lys 210 215 220 His Ile Leu Glu Asp Val Leu Gly Ala AlaPro Gly Ser Val Phe Asn 225 230 235 240 Ala Val Pro Leu Glu Asp Phe GlyGly Gly His Pro Asp Pro Asn Met 245 250 255 Ala His Ala His Glu Leu ThrGlu Ile Met Phe Gly Pro Asn Pro Pro 260 265 270 Val Phe Gly Ala Ala SerAsp Gly Asp Gly Asp Arg Asn Met Ile Met 275 280 285 Gly Ala Asn Ile PheVal Thr Pro Ser Asp Ser Leu Ala Ile Met ala 290 295 300 Ala Asn Ala GlnLeu Ile Pro Ala Tyr Ala Lys Gly Ile Ser Gly Val 305 310 315 320 Ala ArgSer Met Pro Thr Ser Gln Ala Val Asp Arg Val Ala Asp Lys 325 330 335 LeuSer Leu Pro Cys Tyr Glu Thr Pro Thr Gly Trp Lys Phe Phe Gly 340 345 350Asn Leu Leu Asp Ala Asp Lys Ile Thr Leu Cys Gly Glu Glu Ser Phe 355 360365 Gly Ser Gly Ser Asn His Val Arg Glu Lys Asp Gly Leu Trp Ala Val 370375 380 Leu Phe Trp Leu Asn Leu Leu Ala Arg Lys Arg Gln Pro Ala Glu Asp385 390 395 400 Ile Val Arg Glu His Trp Gln Lys Tyr Gly Arg Asp Ile TyrCys Arg 405 410 415 His Asp Tyr Glu Ala Val Asp Ala Asp Ile Ala Asn GlyIle Val Glu 420 425 430 Gln Leu Arg Asn Gln Leu Pro Ser Leu Pro Gly LysThr Trp Gly Asp 435 440 445 Tyr Ser Val Lys Phe Ala Asp Glu Phe Ser TyrThr Asp Pro Val Asp 450 455 460 Gly Ser Val Ser Ser Asn Gln Gly Ile ArgVal Gly Phe Ala Asn Gly 465 470 475 480 Ser Arg Ile Val Phe Arg Leu SerGly Thr Gly Thr Val Gly Ala Thr 485 490 495 Leu Arg Ile Tyr Leu Glu ArgTyr Glu Arg Asp Val Arg Asn His Asp 500 505 510 Gln Asp Pro Gln Val AlaLeu Ala Glu Leu Ile Glu Ile Ala Glu Gln 515 520 525 Leu Cys Gln Val LysGln Arg Thr Gly Arg Thr Glu Pro Ser Val Ile 530 535 540 Thr 545 3 1387DNA METHYLOMONAS SP. 3 4 592 PRT METHYLOMONAS SP. 4 Pro Lys Ala Gly LysIle Thr Val His Phe Phe Leu Ser Ser Val Lys 1 5 10 15 Asp Asn Pro TyrAsn Glu Val Ile Val Leu Leu Ala Thr Ser Gly Thr 20 25 30 Lys Ala Ser GluAsp Ser Leu Ser Gly Ser His Lys Asn Asn Met Ser 35 40 45 Lys Leu Ile AsnSer Ala Glu Trp Asn Ala Val Lys Gln His His Gln 50 55 60 Glu Ile Ala GlyLys Phe Cys Met Lys Glu Ala Phe Ala Lys Asp Pro 65 70 75 80 Gln Arg PheAsp Lys Phe Ser Val Thr Phe Asn Asp Ile Leu Leu Asp 85 90 95 Tyr Ser LysAsn Leu Ile Asp Glu Arg Thr Met Pro Leu Leu Ile Ala 100 105 110 Leu AlaLys Arg Ala Asp Leu Arg Glu Lys Thr Glu Ala Met Phe Ser 115 120 125 GlySer Ile Ile Asn Thr Thr Glu Lys Arg Ala Val Leu His Thr Ala 130 135 140Leu Arg Asn Arg Ser Asn Thr Pro Val Phe Phe Arg Gly Gln Asp Val 145 150155 160 Met Pro Glu Ile Asn Lys Val Leu Ala Lys Met Arg Val Phe Val Glu165 170 175 Gln Val Arg Ser Gly Gln Trp Thr Gly Tyr Ser Gly Lys Ala IleThr 180 185 190 Asp Ile Val Asn Ile Gly Ile Gly Gly Ser Asp Leu Gly ProLys Met 195 200 205 Val Asp Thr Ala Leu Thr Pro Tyr Gly Lys Asn Gly LeuLys Ala His 210 215 220 Phe Val Ser Asn Val Asp Gln Thr Asp Ile Val GluThr Leu Lys Pro 225 230 235 240 Leu Asn Pro Glu Thr Thr Leu Phe Leu IleSer Ser Lys Thr Phe Thr 245 250 255 Thr Gln Glu Thr Met Thr Asn Ala ArgSer Ala Arg Asn Trp Phe Met 260 265 270 Asn Ala Ala Gln Asp Pro Ala HisIle Lys Lys His Phe Ile Ala Ile 275 280 285 Ser Thr Asn Glu Glu Met ValLys Glu Phe Gly Ile Asp Pro Ala Asn 290 295 300 Met Phe Glu Phe Trp AspTrp Val Gly Gly Arg Tyr Ser Leu Trp Ser 305 310 315 320 Val Ile Gly MetSer Ile Ala Leu Tyr Ile Gly Met Asp Asn Phe Glu 325 330 335 Glu Leu LeuMet Gly Ala His Leu Ala Asp Glu His Phe Arg His Ala 340 345 350 Pro TyrGlu Glu Asn Ile Pro Val Ile Met Gly Leu Leu Gly Ile Trp 355 360 365 TyrAsn Asn Phe Phe Glu Ala Glu Thr Tyr Ala Ile Leu Pro Tyr Ala 370 375 380Gln Ser Leu Lys Tyr Phe Ala Asp Tyr Phe Gln Gln Gly Asp Met Glu 385 390395 400 Ser Asn Gly Lys Ser Ala Thr Ile Thr Gly Glu Lys Val Asp Tyr Asn405 410 415 Thr Gly Pro Ile Ile Trp Gly Gln Pro Gly Thr Asn Gly Gln HisAla 420 425 430 Phe Phe Gln Leu Ile His Gln Gly Thr Lys Leu Val Pro GlyAsp Phe 435 440 445 Leu Ala Ala Ala Gln Ser Gln Tyr Asp Leu Pro Asp HisHis Asp Ile 450 455 460 Leu Ile Ser Asn Phe Leu Ala Gln Ala Glu Ala LeuMet Arg Gly Lys 465 470 475 480 Thr Glu Glu Glu Val Arg Gln Asp Leu SerHis Glu Pro Asn Leu Asp 485 490 495 Asp Ala Leu Ile Ala Ser Lys Ile PheGlu Gly Asn Lys Pro Ser Asn 500 505 510 Ser Phe Leu Phe Lys Lys Leu ThrPro Arg Thr Leu Gly Thr Leu Ile 515 520 525 Ala Phe Tyr Glu His Lys IlePhe Val Gln Gly Val Ile Trp Asn Ile 530 535 540 Asn Ser Phe Asp Gln MetGly Val Glu Leu Gly Lys Val Leu Ala Lys 545 550 555 560 Ala Ile Leu ProGlu Leu Lys Asn Asp Asp Ile Ile Ala Ser His Asp 565 570 575 Ser Ser ThrAsn Gly Leu Ile Asn Thr Tyr Lys Arg Leu Arg Lys Ala 580 585 590 5 1311DNA METHYLOMONAS SP. 5 gatgtggtca catggcccta tcacttaacg gctgatattcgattttgtca ttggtttttt 60 cttaacttta acttctacac gctcatgaac aaacctaaaaaagttgcaat actgacagca 120 ggcggcttgg cgccttgttt gaattccgca atcggtagtttgatcgaacg ttataccgaa 180 atcgatccta gcatagaaat catttgctat cgcggcggttataaaggcct gttgctgggc 240 gattcttatc cagtaacggc cgaagtgcgt aaaaaggcgggtgttctgca acgttttggc 300 ggttctgtga tcggcaacag ccgcgtcaaa ttgaccaatgtcaaagactg cgtgaaacgc 360 ggtttggtca aagagggtga agatccgcaa aaagtcgcggctgatcaatt ggttaaggat 420 ggtgtcgata ttctgcacac catcggcggc gatgataccaatacggcagc agcggatttg 480 gcagcattcc tggccagaaa taattacgga ctgaccgtcattggtttacc taaaaccgtc 540 gataacgacg tatttccgat caagcaatca ctaggtgcttggactgccgc cgagcaaggc 600 gcgcgttatt tcatgaacgt ggtggccgaa aacaacgccaacccacgcat gctgatcgta 660 cacgaagtga tgggccgtaa ctgcggctgg ctgaccgctgcaaccgcgca ggaatatcgc 720 aaattactgg accgtgccga gtggttgccg gaattgggtttgactcgtga atcttatgaa 780 gtgcacgcgg tattcgttcc ggaaatggcg atcgacctggaagccgaagc caagcgcctg 840 cgcgaagtga tggacaaagt cgattgcgtc aacatcttcgtttccgaagg tgccggcgtc 900 gaagctatcg tcgcggaaat gcaggccaaa ggccaggaagtgccgcgcga tgcgttcggc 960 cacatcaaac tggatgcggt caaccctggt aaatggttcggcgagcaatt cgcgcagatg 1020 ataggcgcgg aaaaaaccct ggtacaaaaa tcgggatacttcgcccgtgc ttctgcttcc 1080 aacgttgacg acatgcgttt gatcaaatcg tgcgccgacttggcggtcga gtgcgcgttc 1140 cgccgcgagt ctggcgtgat cggtcacgac gaagacaacggcaacgtgtt gcgtgcgatc 1200 gagtttccgc gcatcaaggg cggcaaaccg ttcaatatcgacaccgactg gttcaatagc 1260 atgttgagcg aaatcggcca gcctaaaggc ggtaaagtcgaagtcagcca c 1311 6 437 PRT METHYLOMONAS SP. 6 Asp Val Val Thr Trp ProTyr His Leu Thr Ala Asp Ile Arg Phe Cys 1 5 10 15 His Trp Phe Phe LeuAsn Phe Asn Phe Tyr Thr Leu Met Asn Lys Pro 20 25 30 Lys Lys Val Ala IleLeu Thr Ala Gly Gly Leu Ala Pro Cys Leu Asn 35 40 45 Ser Ala Ile Gly SerLeu Ile Glu Arg Tyr Thr Glu Ile Asp Pro Ser 50 55 60 Ile Glu Ile Ile CysTyr Arg Gly Gly Tyr Lys Gly Leu Leu Leu Gly 65 70 75 80 Asp Ser Tyr ProVal Thr Ala Glu Val Arg Lys Lys Ala Gly Val Leu 85 90 95 Gln Arg Phe GlyGly Ser Val Ile Gly Asn Ser Arg Val Lys Leu Thr 100 105 110 Asn Val LysAsp Cys Val Lys Arg Gly Leu Val Lys Glu Gly Glu Asp 115 120 125 Pro GlnLys Val Ala Ala Asp Gln Leu Val Lys Asp Gly Val Asp Ile 130 135 140 LeuHis Thr Ile Gly Gly Asp Asp Thr Asn Thr Ala Ala Ala Asp Leu 145 150 155160 Ala Ala Phe Leu Ala Arg Asn Asn Tyr Gly Leu Thr Val Ile Gly Leu 165170 175 Pro Lys Thr Val Asp Asn Asp Val Phe Pro Ile Lys Gln Ser Leu Gly180 185 190 Ala Trp Thr Ala Ala Glu Gln Gly Ala Arg Tyr Phe Met Asn ValVal 195 200 205 Ala Glu Asn Asn Ala Asn Pro Arg Met Leu Ile Val His GluVal Met 210 215 220 Gly Arg Asn Cys Gly Trp Leu Thr Ala Ala Thr Ala GlnGlu Tyr Arg 225 230 235 240 Lys Leu Leu Asp Arg Ala Glu Trp Leu Pro GluLeu Gly Leu Thr Arg 245 250 255 Glu Ser Tyr Glu Val His Ala Val Phe ValPro Glu Met ala Ile Asp 260 265 270 Leu Glu Ala Glu Ala Lys Arg Leu ArgGlu Val Met Asp Lys Val Asp 275 280 285 Cys Val Asn Ile Phe Val Ser GluGly Ala Gly Val Glu Ala Ile Val 290 295 300 Ala Glu Met Gln Ala Lys GlyGln Glu Val Pro Arg Asp Ala Phe Gly 305 310 315 320 His Ile Lys Leu AspAla Val Asn Pro Gly Lys Trp Phe Gly Glu Gln 325 330 335 Phe Ala Gln MetIle Gly Ala Glu Lys Thr Leu Val Gln Lys Ser Gly 340 345 350 Tyr Phe AlaArg Ala Ser Ala Ser Asn Val Asp Asp Met Arg Leu Ile 355 360 365 Lys SerCys Ala Asp Leu Ala Val Glu Cys Ala Phe Arg Arg Glu Ser 370 375 380 GlyVal Ile Gly His Asp Glu Asp Asn Gly Asn Val Leu Arg Ala Ile 385 390 395400 Glu Phe Pro Arg Ile Lys Gly Gly Lys Pro Phe Asn Ile Asp Thr Asp 405410 415 Trp Phe Asn Ser Met Leu Ser Glu Ile Gly Gln Pro Lys Gly Gly Lys420 425 430 Val Glu Val Ser His 435 7 1360 DNA METHYLOMONAS SP. 7agtgtcccgc actcgcatca cccggagaca tccttaatgc atcccgtact cgaaaaagtc 60acagaacaag tcatcgcccg cagccgggaa acccgtgccg cttatctgaa gcgcatagag 120gccgccatcg ccgaaggccc gcaacgcaat aaactgcctt gcgccaatct ggcccacggt 180ttcgcggtct gttcggccat cgaaaaagaa gaattgtctc atggccccaa gcccaatgtc 240ggcatcatct cggcctacaa cgacatgctg tccgcgcacg aaccctacaa ggattatcct 300gccctgatca aacaggccgt gcgcgaagcc ggcggcgtgg ctcaattcgc cggcggcgtg 360cccgcgatgt gcgacggcgt cacccagggc atgccgggca tggaattgtc gctattcagc 420cgcgacgtca tcgcgatgtc caccgcgatc ggcctggctc ataacatgtt cgacgcggcg 480ctgtatctgg gcgtctgcga caagatcgta cccggtttgt tgatcggtgc attgagcttc 540ggccatttgc cggccgtttt cttgccagcc ggccccatga ccagcggcct gtccaacaag 600gaaaaatccc gtgcccggca aaaatacgcc gaaggtaaga tcggtgaaaa agaattgctg 660gaatcggaag ccaagtctta ccacagccca ggcacctgca ccttctatgg caccgccaac 720agcaaccaga tgatggtcga gatcatgggc ctgcacctgc ccggtagttc cttcatcaat 780ccttacaccc cactgcgcga cgaactgacc aaggccgccg ccaggcaggt gttgaaattc 840accgcgctgg gcaacgactt caggccaatc gcgcatgtga tcgacgaaaa agccatcatc 900aatgccatca tcggcttgct ggcgaccggc ggttcgacca accataccat ccatttgatc 960gcgattgccc gcgccgccgg catcatcatc aactgggacg atttcgacgc cctatccaaa 1020gtcattccgt tgctgaccaa gatctatccg aacggcccgg ccgacgtcaa ccaattccag 1080gcggccggcg gcatgagctt attgatacac gaactgctgg atcacggctt gttgcacggc 1140gacatcctga ccataggcga ccagcgcggc atggcccaat acagtcaagt accgacgctg 1200caagacggcc aattacaatg gcagcccggt cctaccgcat cgcgcgatcc cgaaatcatc 1260gccagcgtgg caaaaccttt cgccgccggt ggtggcctgc atgtgatgca tggcaatctg 1320ggccgcggcg tatccaagat ttccgccgtc tccgaagatc 1360 8 618 PRT METHYLOMONASSP. 8 Ser Val Pro His Ser His His Pro Glu Thr Ser Leu Met His Pro Val 15 10 15 Leu Glu Lys Val Thr Glu Gln Val Ile Ala Arg Ser Arg Glu Thr Arg20 25 30 Ala Ala Tyr Leu Lys Arg Ile Glu Ala Ala Ile Ala Glu Gly Pro Gln35 40 45 Arg Asn Lys Leu Pro Cys Ala Asn Leu Ala His Gly Phe Ala Val Cys50 55 60 Ser Ala Ile Glu Lys Glu Glu Leu Ser His Gly Pro Lys Pro Asn Val65 70 75 80 Gly Ile Ile Ser Ala Tyr Asn Asp Met Leu Ser Ala His Glu ProTyr 85 90 95 Lys Asp Tyr Pro Ala Leu Ile Lys Gln Ala Val Arg Glu Ala GlyGly 100 105 110 Val Ala Gln Phe Ala Gly Gly Val Pro Ala Met Cys Asp GlyVal Thr 115 120 125 Gln Gly Met Pro Gly Met Glu Leu Ser Leu Phe Ser ArgAsp Val Ile 130 135 140 Ala Met Ser Thr Ala Ile Gly Leu Ala His Asn MetPhe Asp Ala Ala 145 150 155 160 Leu Tyr Leu Gly Val Cys Asp Lys Ile ValPro Gly Leu Leu Ile Gly 165 170 175 Ala Leu Ser Phe Gly His Leu Pro AlaVal Phe Leu Pro Ala Gly Pro 180 185 190 Met Thr Ser Gly Leu Ser Asn LysGlu Lys Ser Arg Ala Arg Gln Lys 195 200 205 Tyr Ala Glu Gly Lys Ile GlyGlu Lys Glu Leu Leu Glu Ser Glu Ala 210 215 220 Lys Ser Tyr His Ser ProGly Thr Cys Thr Phe Tyr Gly Thr Ala Asn 225 230 235 240 Ser Asn Gln MetMet Val Glu Ile Met Gly Leu His Leu Pro Gly Ser 245 250 255 Ser Phe IleAsn Pro Tyr Thr Pro Leu Arg Asp Glu Leu Thr Lys Ala 260 265 270 Ala AlaArg Gln Val Leu Lys Phe Thr Ala Leu Gly Asn Asp Phe Arg 275 280 285 ProIle Ala His Val Ile Asp Glu Lys Ala Ile Ile Asn Ala Ile Ile 290 295 300Gly Leu Leu Ala Thr Gly Gly Ser Thr Asn His Thr Ile His Leu Ile 305 310315 320 Ala Ile Ala Arg Ala Ala Gly Ile Ile Ile Asn Trp Asp Asp Phe Asp325 330 335 Ala Leu Ser Lys Val Ile Pro Leu Leu Thr Lys Ile Tyr Pro AsnGly 340 345 350 Pro Ala Asp Val Asn Gln Phe Gln Ala Ala Gly Gly Met SerLeu Leu 355 360 365 Ile His Glu Leu Leu Asp His Gly Leu Leu His Gly AspIle Leu Thr 370 375 380 Ile Gly Asp Gln Arg Gly Met ala Gln Tyr Ser GlnVal Pro Thr Leu 385 390 395 400 Gln Asp Gly Gln Leu Gln Trp Gln Pro GlyPro Thr Ala Ser Arg Asp 405 410 415 Pro Glu Ile Ile Ala Ser Val Ala LysPro Phe Ala Ala Gly Gly Gly 420 425 430 Leu His Val Met His Gly Asn LeuGly Arg Gly Val Ser Lys Ile Ser 435 440 445 Ala Val Ser Glu Asp His GlnVal Val Thr Ala Pro Ala Met Val Phe 450 455 460 Asp Asp Gln Leu Asp ValVal Ala Ala Phe Lys Arg Gly Glu Leu Glu 465 470 475 480 Lys Asp Val IleVal Val Leu Arg Phe Gln Gly Pro Lys Ala Asn Gly 485 490 495 Met Pro GluLeu His Lys Leu Thr Pro Val Leu Gly Val Leu Gln Asp 500 505 510 Arg GlyPhe Lys Val Gly Leu Leu Thr Asp Gly Arg Met Ser Gly Ala 515 520 525 SerGly Lys Val Pro Ser Ala Ile His Met Trp Pro Glu Cys Ile Asp 530 535 540Gly Gly Pro Leu Ala Lys Val Arg Asp Gly Asp Ile Ile Val Met Asn 545 550555 560 Thr Gln Thr Gly Glu Val Asn Val Gln Val Asp Pro Ala Glu Phe Lys565 570 575 Ala Arg Val Ala Glu Pro Asn His Ala Thr Gly His His Phe GlyMet 580 585 590 Gly Arg Glu Leu Phe Gly Ala Met Arg Ala Gln Ala Ser ThrAla Glu 595 600 605 Thr Gly Ala Thr Asn Leu Phe Phe Val Asp 610 615 91477 DNA METHYLOMONAS SP. 9 atggcattgg gctttttgct ccgtagcccc aaagacatgacaaaaaacat tacttacaaa 60 ccctgcgacc tggtgattta cggcgcactg ggcgatttatccaaacgtaa actactgatt 120 tcattatacc gtttggaaaa acacaatctg ctcgagcccgatacgcgcat catcggcgta 180 gatcgtttgg aagaaaccag cgacagtttc gtcgaaattgcgcacaaaag cttgcaggcg 240 tttttgaaca acgtcatcga cgcagaaatc tggcaacgtttttccaaacg cttgtcctat 300 ttgaaaatcg atctgaccca acccgagcaa tacaaacaactgcatacggt cgtcgatgcc 360 gaaaaacgag tcatggtgaa ttatttcgcg gtggcaccctttttgttcaa aaacatttgc 420 caaggcttgc atgactgcgg cgtattgacg gccgaatcgcgcatggtgat ggaaaaaccc 480 atcggccacg acctgaaatc gtcgaaagaa atcaacgacgtcgtcgccga cgtattccac 540 gaagaccagg tctaccgcat cgaccactac ctgggcaaggaaacggtctt gaacttgctg 600 gccttgcgtt tcgccaattc gatattcacg accaactggaatcacaacac gatagaccat 660 atccagatta cggtcggtga ggacatcggc atcgagggccgttgggaata tttcgacaag 720 accggccaat tgcgcgacat gctgcaaaac catttgctgcaaatcctgac cttcgtcgcg 780 atggagccgc ccgcggatct gtcggccgaa agcatacacatggaaaaaat caaggtcctg 840 aaagccttgc ggccaatcac cgtgcgcaat gtcgaggaaaaaaccgtgcg cggtcaatac 900 accgccggtt tcatcaaagg caagtcggta ccgggttatctggaagaaga aggtgccaac 960 accgaaagca cgaccgaaac tttcgtcgcg atccgcgtggatatcgataa ctggcgctgg 1020 gccggtgtcc cgttttacat gcgtaccggc aaacgcacgcccaacaaacg caccgagatt 1080 gtggtcaatt tcaagcaatt gccgcacaac atcttcaaggacagttttca tgaactgccg 1140 gccaataaac tggtcattca tttgcaaccg aacgaaggggtggatgtcat gatgttgaac 1200 aaggtgccgg gcatagacgg caacatcaag ttgcaacagaccaaactgga tttgagcttt 1260 tccgaaacct tcaagaaaaa ccgaattttc ggcggctacgaaaaactgat tctggaagcc 1320 ctgcgcggca acccgacgct gtttttgagc cgcgaggaaatagaacaagc ctggacctgg 1380 gtcgattcga ttcaggatgc ctggcaacac aaccacacgccacccaaacc ctatcccgcg 1440 ggtagctggg gtccagtggc atcggtcgca ttactgg 147710 501 PRT METHYLOMONAS SP. 10 Met ala Leu Gly Phe Leu Leu Arg Ser ProLys Asp Met Thr Lys Asn 1 5 10 15 Ile Thr Tyr Lys Pro Cys Asp Leu ValIle Tyr Gly Ala Leu Gly Asp 20 25 30 Leu Ser Lys Arg Lys Leu Leu Ile SerLeu Tyr Arg Leu Glu Lys His 35 40 45 Asn Leu Leu Glu Pro Asp Thr Arg IleIle Gly Val Asp Arg Leu Glu 50 55 60 Glu Thr Ser Asp Ser Phe Val Glu IleAla His Lys Ser Leu Gln Ala 65 70 75 80 Phe Leu Asn Asn Val Ile Asp AlaGlu Ile Trp Gln Arg Phe Ser Lys 85 90 95 Arg Leu Ser Tyr Leu Lys Ile AspLeu Thr Gln Pro Glu Gln Tyr Lys 100 105 110 Gln Leu His Thr Val Val AspAla Glu Lys Arg Val Met Val Asn Tyr 115 120 125 Phe Ala Val Ala Pro PheLeu Phe Lys Asn Ile Cys Gln Gly Leu His 130 135 140 Asp Cys Gly Val LeuThr Ala Glu Ser Arg Met Val Met Glu Lys Pro 145 150 155 160 Ile Gly HisAsp Leu Lys Ser Ser Lys Glu Ile Asn Asp Val Val Ala 165 170 175 Asp ValPhe His Glu Asp Gln Val Tyr Arg Ile Asp His Tyr Leu Gly 180 185 190 LysGlu Thr Val Leu Asn Leu Leu Ala Leu Arg Phe Ala Asn Ser Ile 195 200 205Phe Thr Thr Asn Trp Asn His Asn Thr Ile Asp His Ile Gln Ile Thr 210 215220 Val Gly Glu Asp Ile Gly Ile Glu Gly Arg Trp Glu Tyr Phe Asp Lys 225230 235 240 Thr Gly Gln Leu Arg Asp Met Leu Gln Asn His Leu Leu Gln IleLeu 245 250 255 Thr Phe Val Ala Met Glu Pro Pro Ala Asp Leu Ser Ala GluSer Ile 260 265 270 His Met Glu Lys Ile Lys Val Leu Lys Ala Leu Arg ProIle Thr Val 275 280 285 Arg Asn Val Glu Glu Lys Thr Val Arg Gly Gln TyrThr Ala Gly Phe 290 295 300 Ile Lys Gly Lys Ser Val Pro Gly Tyr Leu GluGlu Glu Gly Ala Asn 305 310 315 320 Thr Glu Ser Thr Thr Glu Thr Phe ValAla Ile Arg Val Asp Ile Asp 325 330 335 Asn Trp Arg Trp Ala Gly Val ProPhe Tyr Met Arg Thr Gly Lys Arg 340 345 350 Thr Pro Asn Lys Arg Thr GluIle Val Val Asn Phe Lys Gln Leu Pro 355 360 365 His Asn Ile Phe Lys AspSer Phe His Glu Leu Pro Ala Asn Lys Leu 370 375 380 Val Ile His Leu GlnPro Asn Glu Gly Val Asp Val Met Met Leu Asn 385 390 395 400 Lys Val ProGly Ile Asp Gly Asn Ile Lys Leu Gln Gln Thr Lys Leu 405 410 415 Asp LeuSer Phe Ser Glu Thr Phe Lys Lys Asn Arg Ile Phe Gly Gly 420 425 430 TyrGlu Lys Leu Ile Leu Glu Ala Leu Arg Gly Asn Pro Thr Leu Phe 435 440 445Leu Ser Arg Glu Glu Ile Glu Gln Ala Trp Thr Trp Val Asp Ser Ile 450 455460 Gln Asp Ala Trp Gln His Asn His Thr Pro Pro Lys Pro Tyr Pro Ala 465470 475 480 Gly Ser Trp Gly Pro Val Ala Ser Val Ala Leu Leu Ala Arg AspGly 485 490 495 Arg Ala Trp Glu Glu 500 11 984 DNA METHYLOMONAS SP. 11atggcaagaa acttacttga gcaactccgc gagatgaccg ttgttgttgc cgataccggt 60gacatccagg cgatcgaaac cttcaagccg cgcgatgcaa cgaccaaccc gtctttgatc 120accgccgcgg cgcaaatgcc gcaatatcaa ggcatcgttg acgacacctt gaaaggtgcg 180cgtgcgacgt tgggtgccag cgcttcggct gccgaggtgg cttcattggc gttcgatcgt 240ttggcggttt ctttcggttt gaaaatcctg gaaatcatcg aaggtcgcgt ttccaccgag 300gttgatgcgc gtttgtctta tgacaccgaa ggcactattg ccaaaggccg ggatctgatc 360aaacaatacg aagctgcagg tgtttccaaa gagcgcgtac tgatcaaaat tgccgcgacc 420tgggaaggca tccaggcggc tgccgttttg gaaaaagaag gtattcacac caacttgacc 480ctgttgttcg gtctgcacca ggcgattgct tgtgccgaaa acggcattac cctgatttct 540ccgtttgtcg gccgtattct ggactggtac aaaaaagaca ctggccgcga ctcttatcct 600tccaacgaag atcctggcgt attgtctgta actgaagttt ataactacta caaaaaattt 660ggttataaaa ctgaagtcat gggcgcgagc ttccgtaaca tcggcgaaat caccgaattg 720gcgggttgcg atctgttgac catcgcgcct tctctgctgg ccgaactgca atccgttgaa 780ggtgatttgc cacgcaaact ggaccctgca aaagcagccg gttcttcgat cgaaaaaatc 840agcgttgaca aagcgacttt cgagcgcatg cacgaagaaa accgcatggc caaagaaaaa 900ctggccgaag gtatcgacgg ttttgcgaaa gcgttggaaa ccttggaaaa attgttggcg 960gatcgtttgg ctgctctgga agca 984 12 328 PRT METHYLOMONAS SP. 12 Met alaArg Asn Leu Leu Glu Gln Leu Arg Glu Met Thr Val Val Val 1 5 10 15 AlaAsp Thr Gly Asp Ile Gln Ala Ile Glu Thr Phe Lys Pro Arg Asp 20 25 30 AlaThr Thr Asn Pro Ser Leu Ile Thr Ala Ala Ala Gln Met Pro Gln 35 40 45 TyrGln Gly Ile Val Asp Asp Thr Leu Lys Gly Ala Arg Ala Thr Leu 50 55 60 GlyAla Ser Ala Ser Ala Ala Glu Val Ala Ser Leu Ala Phe Asp Arg 65 70 75 80Leu Ala Val Ser Phe Gly Leu Lys Ile Leu Glu Ile Ile Glu Gly Arg 85 90 95Val Ser Thr Glu Val Asp Ala Arg Leu Ser Tyr Asp Thr Glu Gly Thr 100 105110 Ile Ala Lys Gly Arg Asp Leu Ile Lys Gln Tyr Glu Ala Ala Gly Val 115120 125 Ser Lys Glu Arg Val Leu Ile Lys Ile Ala Ala Thr Trp Glu Gly Ile130 135 140 Gln Ala Ala Ala Val Leu Glu Lys Glu Gly Ile His Thr Asn LeuThr 145 150 155 160 Leu Leu Phe Gly Leu His Gln Ala Ile Ala Cys Ala GluAsn Gly Ile 165 170 175 Thr Leu Ile Ser Pro Phe Val Gly Arg Ile Leu AspTrp Tyr Lys Lys 180 185 190 Asp Thr Gly Arg Asp Ser Tyr Pro Ser Asn GluAsp Pro Gly Val Leu 195 200 205 Ser Val Thr Glu Val Tyr Asn Tyr Tyr LysLys Phe Gly Tyr Lys Thr 210 215 220 Glu Val Met Gly Ala Ser Phe Arg AsnIle Gly Glu Ile Thr Glu Leu 225 230 235 240 Ala Gly Cys Asp Leu Leu ThrIle Ala Pro Ser Leu Leu Ala Glu Leu 245 250 255 Gln Ser Val Glu Gly AspLeu Pro Arg Lys Leu Asp Pro Ala Lys Ala 260 265 270 Ala Gly Ser Ser IleGlu Lys Ile Ser Val Asp Lys Ala Thr Phe Glu 275 280 285 Arg Met His GluGlu Asn Arg Met ala Lys Glu Lys Leu Ala Glu Gly 290 295 300 Ile Asp GlyPhe Ala Lys Ala Leu Glu Thr Leu Glu Lys Leu Leu Ala 305 310 315 320 AspArg Leu Ala Ala Leu Glu Ala 325 13 480 DNA METHYLOMONAS SP. 13atggccgcgg gcggcgtggg cttgacgcaa ttgctgccag aactggccga agctattggt 60ccgacgagcc gatttcatgt gcaggtcatt ggtgacacgg tggaggacat cgttgcggaa 120gccaaacggc tacacgattt gcccgtcgac atagtggtga aaattccggc gcatggcgcc 180ggactggcgg ccatcaagca gatcaagcgc cacgatattc cggtgctggc gacagcgatt 240tacaacgtgc agcaaggttg gctggcggct ttgaacggcg ccgattatct ggcgccttat 300ctgaatcgcg tcgataacca gggttttgac ggtattggcg tggtcgccga tctgcagagc 360ttgatcgacc ggtatcaaat gcccaccaaa ctcctggtag cgagcttcaa aaacgtacaa 420caggtgctgc aggtgttgaa actgggcgtg gcgtcggtga cgctgccttt ggacattgtg 480 14160 PRT METHYLOMONAS SP. 14 Met ala Ala Gly Gly Val Gly Leu Thr Gln LeuLeu Pro Glu Leu Ala 1 5 10 15 Glu Ala Ile Gly Pro Thr Ser Arg Phe HisVal Gln Val Ile Gly Asp 20 25 30 Thr Val Glu Asp Ile Val Ala Glu Ala LysArg Leu His Asp Leu Pro 35 40 45 Val Asp Ile Val Val Lys Ile Pro Ala HisGly Ala Gly Leu Ala Ala 50 55 60 Ile Lys Gln Ile Lys Arg His Asp Ile ProVal Leu Ala Thr Ala Ile 65 70 75 80 Tyr Asn Val Gln Gln Gly Trp Leu AlaAla Leu Asn Gly Ala Asp Tyr 85 90 95 Leu Ala Pro Tyr Leu Asn Arg Val AspAsn Gln Gly Phe Asp Gly Ile 100 105 110 Gly Val Val Ala Asp Leu Gln SerLeu Ile Asp Arg Tyr Gln Met Pro 115 120 125 Thr Lys Leu Leu Val Ala SerPhe Lys Asn Val Gln Gln Val Leu Gln 130 135 140 Val Leu Lys Leu Gly ValAla Ser Val Thr Leu Pro Leu Asp Ile Val 145 150 155 160 15 1005 DNAMETHYLOMONAS SP. 15 atggctttag tgtcattgcg acaacttttg gattatgcggccgagcatgg ctttgccgtg 60 ccggcgttca acgtcagcaa catggagcag gtacaggccatcatgcaggc ggccgctgcc 120 tgcgatagtc cagtgatcat gcaaggttcg gccggcgccaaccgctatgc cggcgaagtg 180 tttctacggc atttgatatt ggcggccgtg gagcaatatccgcatattcc ggtcgtcatg 240 caccgcgacc atgcacccac gcccgacatc tgcgcgcaagccatacaatc gggcttcagc 300 tcggtgatga tggacggttc gttgctggca gacatgaaaaccccggcttc ttttgcatac 360 aacgtcgacg tcacccgcac cgtggtcaag atggcgcatgcctgcggcgt atcggtggaa 420 ggcgaaatcg gctgcctggg agcgctggag gccaagtccgcgcaagatca cagccgtttg 480 ctgaccgatc ccgacgaagc ggtcgaattc gtcgaacagacccaggtcga tgccgtggcc 540 gtggccatcg gcaccagcca cggcgcctat aaattcagcaagccgcccac cggcgaagtg 600 ctggtgatca gtcgattgaa agaactgcag caacgactgccaaataccca ttttgtgatg 660 catggctcca gttcggtgcc gcaggattgg ttgaaaatcatcaacgatta tggcggcgat 720 attccggaaa cctatggcgt gccggtcgaa gaaatcgtcgaaggcataaa atatggtgtg 780 cgcaaggtca acatcgatac cgacctgcgc atggcgtccaccggcgcgat gcgcaggttt 840 ctggcccaac cggaaaacgc ctcggagcta gacgcgcgcaagacctatca agccgccagg 900 gacgcaatgc aggcattatg ccaggctcgc tacgaagcgttcggttcggc gggacatgcc 960 ggcaaaatca aaccggtttc actggcggca atggccaaacgctat 1005 16 335 PRT METHYLOMONAS SP. 16 Met ala Leu Val Ser Leu ArgGln Leu Leu Asp Tyr Ala Ala Glu His 1 5 10 15 Gly Phe Ala Val Pro AlaPhe Asn Val Ser Asn Met Glu Gln Val Gln 20 25 30 Ala Ile Met Gln Ala AlaAla Ala Cys Asp Ser Pro Val Ile Met Gln 35 40 45 Gly Ser Ala Gly Ala AsnArg Tyr Ala Gly Glu Val Phe Leu Arg His 50 55 60 Leu Ile Leu Ala Ala ValGlu Gln Tyr Pro His Ile Pro Val Val Met 65 70 75 80 His Arg Asp His AlaPro Thr Pro Asp Ile Cys Ala Gln Ala Ile Gln 85 90 95 Ser Gly Phe Ser SerVal Met Met Asp Gly Ser Leu Leu Ala Asp Met 100 105 110 Lys Thr Pro AlaSer Phe Ala Tyr Asn Val Asp Val Thr Arg Thr Val 115 120 125 Val Lys Metala His Ala Cys Gly Val Ser Val Glu Gly Glu Ile Gly 130 135 140 Cys LeuGly Ala Leu Glu Ala Lys Ser Ala Gln Asp His Ser Arg Leu 145 150 155 160Leu Thr Asp Pro Asp Glu Ala Val Glu Phe Val Glu Gln Thr Gln Val 165 170175 Asp Ala Val Ala Val Ala Ile Gly Thr Ser His Gly Ala Tyr Lys Phe 180185 190 Ser Lys Pro Pro Thr Gly Glu Val Leu Val Ile Ser Arg Leu Lys Glu195 200 205 Leu Gln Gln Arg Leu Pro Asn Thr His Phe Val Met His Gly SerSer 210 215 220 Ser Val Pro Gln Asp Trp Leu Lys Ile Ile Asn Asp Tyr GlyGly Asp 225 230 235 240 Ile Pro Glu Thr Tyr Gly Val Pro Val Glu Glu IleVal Glu Gly Ile 245 250 255 Lys Tyr Gly Val Arg Lys Val Asn Ile Asp ThrAsp Leu Arg Met ala 260 265 270 Ser Thr Gly Ala Met Arg Arg Phe Leu AlaGln Pro Glu Asn Ala Ser 275 280 285 Glu Leu Asp Ala Arg Lys Thr Tyr GlnAla Ala Arg Asp Ala Met Gln 290 295 300 Ala Leu Cys Gln Ala Arg Tyr GluAla Phe Gly Ser Ala Gly His Ala 305 310 315 320 Gly Lys Ile Lys Pro ValSer Leu Ala Ala Met ala Lys Arg Tyr 325 330 335 17 1074 DNA METHYLOMONASSP. 17 atgacaaaaa tcttagatgt tgtaaaaccc ggcgttgtca ccggtgaaga tgtgcaaaaa60 attttcgcaa tctgcaaaga aaacaacttt gccttgccag ccgtcaacgt gatcagtacc 120gataccatta atgcggtatt ggaagcggcc gccaaagcca aatcacctgt tgttatccag 180ttttcaaatg gcggcgcggc tttcgttgcc ggtaaaggtt tgaaattgga aggtcaaggc 240tgttcgattc atggtgccat ttcaggtgct caccacgttc accgcttggc ggaactctat 300ggtgtacctg tcgttctgca taccgaccac gcggcgaaaa aattgctgcc atgggtagat 360ggtatgctgg atgaaggtga aaaattcttt gcggccaccg gcaagccttt gttcagctcg 420cacatgctgg acttgtccga agagagcctg gaagaaaaca tcgaaatctg cggtaaatac 480ttggcgcgca tggcgaaaat gggtatgacc ttggaaatcg aactgggctg caccggcggt 540gaagaagacg gcgtggacaa cagcggcatg gatcattccg cgttgtacac ccagccggaa 600gacgtggctt acgcgtatga gcacctgagc aaaatcagcc ctaacttcac gattgcggct 660tctttcggca acgtgcacgg cgtttactcg ccaggaaacg tcaagctgac gccaaaaatt 720ctggataact cgcaaaaata cgtatccgaa aaattcggct tgccagctaa atcattgacc 780ttcgtattcc atggcggctc tggttcgtct ccggaagaaa tcaaggaatc catcagctat 840ggcgtagtga aaatgaacat cgataccgat acccaatggg caacctggga aggcgtgatg 900aacttctaca agaaaaacga aggctatctg caaggccaga tcggcaatcc ggaaggtgcc 960gacaagccga acaaaaaata ctatgaccca cgcgtatggc aacgtgccgg ccaagaaggc 1020atggttgcac gtctgcaaca agcattccag gaattgaatg cagtaaacac gctg 1074 18 358PRT METHYLOMONAS SP. 18 Met Thr Lys Ile Leu Asp Val Val Lys Pro Gly ValVal Thr Gly Glu 1 5 10 15 Asp Val Gln Lys Ile Phe Ala Ile Cys Lys GluAsn Asn Phe Ala Leu 20 25 30 Pro Ala Val Asn Val Ile Ser Thr Asp Thr IleAsn Ala Val Leu Glu 35 40 45 Ala Ala Ala Lys Ala Lys Ser Pro Val Val IleGln Phe Ser Asn Gly 50 55 60 Gly Ala Ala Phe Val Ala Gly Lys Gly Leu LysLeu Glu Gly Gln Gly 65 70 75 80 Cys Ser Ile His Gly Ala Ile Ser Gly AlaHis His Val His Arg Leu 85 90 95 Ala Glu Leu Tyr Gly Val Pro Val Val LeuHis Thr Asp His Ala Ala 100 105 110 Lys Lys Leu Leu Pro Trp Val Asp GlyMet Leu Asp Glu Gly Glu Lys 115 120 125 Phe Phe Ala Ala Thr Gly Lys ProLeu Phe Ser Ser His Met Leu Asp 130 135 140 Leu Ser Glu Glu Ser Leu GluGlu Asn Ile Glu Ile Cys Gly Lys Tyr 145 150 155 160 Leu Ala Arg Met alaLys Met Gly Met Thr Leu Glu Ile Glu Leu Gly 165 170 175 Cys Thr Gly GlyGlu Glu Asp Gly Val Asp Asn Ser Gly Met Asp His 180 185 190 Ser Ala LeuTyr Thr Gln Pro Glu Asp Val Ala Tyr Ala Tyr Glu His 195 200 205 Leu SerLys Ile Ser Pro Asn Phe Thr Ile Ala Ala Ser Phe Gly Asn 210 215 220 ValHis Gly Val Tyr Ser Pro Gly Asn Val Lys Leu Thr Pro Lys Ile 225 230 235240 Leu Asp Asn Ser Gln Lys Tyr Val Ser Glu Lys Phe Gly Leu Pro Ala 245250 255 Lys Ser Leu Thr Phe Val Phe His Gly Gly Ser Gly Ser Ser Pro Glu260 265 270 Glu Ile Lys Glu Ser Ile Ser Tyr Gly Val Val Lys Met Asn IleAsp 275 280 285 Thr Asp Thr Gln Trp Ala Thr Trp Glu Gly Val Met Asn PheTyr Lys 290 295 300 Lys Asn Glu Gly Tyr Leu Gln Gly Gln Ile Gly Asn ProGlu Gly Ala 305 310 315 320 Asp Lys Pro Asn Lys Lys Tyr Tyr Asp Pro ArgVal Trp Gln Arg Ala 325 330 335 Gly Gln Glu Gly Met Val Ala Arg Leu GlnGln Ala Phe Gln Glu Leu 340 345 350 Asn Ala Val Asn Thr Leu 355 19 636DNA METHYLOMONAS SP. 19 gaaaatacta tgtccgtcac catcaaagaa gtcatgaccacctcgcccgt tatgccggtc 60 atggtcatca atcatctgga acatgccgtc cctctggctcgcgcgctagt cgacggtggc 120 ttgaaagttt tggagatcac attgcgcacg ccggtggcactggaatgtat ccgacgtatc 180 aaagccgaag taccggacgc catcgtcggc gcgggcaccatcatcaaccc tcataccttg 240 tatcaagcga ttgacgccgg tgcggaattc atcgtcagccccggcatcac cgaaaatcta 300 ctcaacgaag cgctagcatc cggcgtgcct atcctgcccggcgtcatcac acccagcgag 360 gtcatgcgtt tattggaaaa aggcatcaat gcgatgaaattctttccggc tgaagccgcc 420 ggcggcatac cgatgctgaa atcccttggc ggccccttgccgcaagtcac cttctgtccg 480 accggcggcg tcaatcccaa aaacgcgccc gaatatctggcattgaaaaa tgtcgcctgc 540 gtcggcggct cctggatggc gccggccgat ctggtagatgccgaagactg ggcggaaatc 600 acgcggcggg cgagcgaggc cgcggcattg aaaaaa 636 20212 PRT METHYLOMONAS SP. 20 Glu Asn Thr Met Ser Val Thr Ile Lys Glu ValMet Thr Thr Ser Pro 1 5 10 15 Val Met Pro Val Met Val Ile Asn His LeuGlu His Ala Val Pro Leu 20 25 30 Ala Arg Ala Leu Val Asp Gly Gly Leu LysVal Leu Glu Ile Thr Leu 35 40 45 Arg Thr Pro Val Ala Leu Glu Cys Ile ArgArg Ile Lys Ala Glu Val 50 55 60 Pro Asp Ala Ile Val Gly Ala Gly Thr IleIle Asn Pro His Thr Leu 65 70 75 80 Tyr Gln Ala Ile Asp Ala Gly Ala GluPhe Ile Val Ser Pro Gly Ile 85 90 95 Thr Glu Asn Leu Leu Asn Glu Ala LeuAla Ser Gly Val Pro Ile Leu 100 105 110 Pro Gly Val Ile Thr Pro Ser GluVal Met Arg Leu Leu Glu Lys Gly 115 120 125 Ile Asn Ala Met Lys Phe PhePro Ala Glu Ala Ala Gly Gly Ile Pro 130 135 140 Met Leu Lys Ser Leu GlyGly Pro Leu Pro Gln Val Thr Phe Cys Pro 145 150 155 160 Thr Gly Gly ValAsn Pro Lys Asn Ala Pro Glu Tyr Leu Ala Leu Lys 165 170 175 Asn Val AlaCys Val Gly Gly Ser Trp Met ala Pro Ala Asp Leu Val 180 185 190 Asp AlaGlu Asp Trp Ala Glu Ile Thr Arg Arg Ala Ser Glu Ala Ala 195 200 205 AlaLeu Lys Lys 210 21 873 DNA Methylomonas 16a 21 atgaaagtta ccaaagccgtttttcccgtt gccggactgg gcacccggtc attgcccgca 60 accaaggccg ttgccaaggaaatgttgccg gtggtggaca agccgctgat tcagtatgcg 120 gtggaagagg ccgtggccgccggcatcgac acgatgattt tcgtgatcgg tagaaacaag 180 gaatccattg ccaaccatttcgataaatcc tacgaactgg aaaaggaact ggaaaaaagc 240 ggcaagaccg atttgctgaaaatgctgcgg gagattttgc ccgcgcatgt gtcctgcgta 300 ttcgtgcgtc aagcggaggctctgggtttg gggcatgcgg tgcattgcgc caagccggtg 360 gtcggcaacg agccgtttgcggtgatcttg ccggatgact tgatcgagga cggcgagcgc 420 ggttgcatga agcagatggtggatttgttc gacaaagagc aaagcagcgt attgggggta 480 gagcgggtcg atcccaaggaaacccataag tacggcatcg tcgaacatgc cgaaacctcg 540 cccagagtcg gttggttgagttccatcgtc gagaaaccca aacccgaagt ggcgccctcc 600 aatatcgcgg tggtcgggcgctacatcttg acgccggcca tttttcaaaa aatcgagaac 660 acggggcgcg gcgccggcggcgaaattcaa ttgaccgatg cgattgccgc gttgatgaaa 720 gacgaacgcg ttttgtcctatgaattcgaa ggcaatcgct acgactgcgg ttccaagttt 780 ggttttttgt tggccaatgtcgaatatggc ttgctgcaca aggaaatcaa agccgaattc 840 gccaactatc tgaaacaacgcgtcagcaaa atc 873 22 293 PRT Methylomonas 16a 22 Met Thr Met Lys ValThr Lys Ala Val Phe Pro Val Ala Gly Leu Gly 1 5 10 15 Thr Arg Ser LeuPro Ala Thr Lys Ala Val Ala Lys Glu Met Leu Pro 20 25 30 Val Val Asp LysPro Leu Ile Gln Tyr Ala Val Glu Glu Ala Val Ala 35 40 45 Ala Gly Ile AspThr Met Ile Phe Val Ile Gly Arg Asn Lys Glu Ser 50 55 60 Ile Ala Asn HisPhe Asp Lys Ser Tyr Glu Leu Glu Lys Glu Leu Glu 65 70 75 80 Lys Ser GlyLys Thr Asp Leu Leu Lys Met Leu Arg Glu Ile Leu Pro 85 90 95 Ala His ValSer Cys Val Phe Val Arg Gln Ala Glu Ala Leu Gly Leu 100 105 110 Gly HisAla Val His Cys Ala Lys Pro Val Val Gly Asn Glu Pro Phe 115 120 125 AlaVal Ile Leu Pro Asp Asp Leu Ile Glu Asp Gly Glu Arg Gly Cys 130 135 140Met Lys Gln Met Val Asp Leu Phe Asp Lys Glu Gln Ser Ser Val Leu 145 150155 160 Gly Val Glu Arg Val Asp Pro Lys Glu Thr His Lys Tyr Gly Ile Val165 170 175 Glu His Ala Glu Thr Ser Pro Arg Val Gly Trp Leu Ser Ser IleVal 180 185 190 Glu Lys Pro Lys Pro Glu Val Ala Pro Ser Asn Ile Ala ValVal Gly 195 200 205 Arg Tyr Ile Leu Thr Pro Ala Ile Phe Gln Lys Ile GluAsn Thr Gly 210 215 220 Arg Gly Ala Gly Gly Glu Ile Gln Leu Thr Asp AlaIle Ala Ala Leu 225 230 235 240 Met Lys Asp Glu Arg Val Leu Ser Tyr GluPhe Glu Gly Asn Arg Tyr 245 250 255 Asp Cys Gly Ser Lys Phe Gly Phe LeuLeu Ala Asn Val Glu Tyr Gly 260 265 270 Leu Leu His Lys Glu Ile Lys AlaGlu Phe Ala Asn Tyr Leu Lys Gln 275 280 285 Arg Val Ser Lys Ile 290 231419 DNA Methylomonas 16a 23 atgccactcg gtttgggaaa tatcttcaac gggctgttcaagcaatacgg gcacacggtg 60 atcctgttgt tgagggttat cgacgtggtc atgttattgggcgcggcctg gctggcgcat 120 tatttttggt tgcatgacag cgtcatcgat cagcattaccgtttcgtgat tgccctgggt 180 atcttgggtg cgatcatatt tttcgagatc ggccaggtgtatcggccgtg gcgcaatgac 240 gcgatgcgcg gcgaaattcc ccgcatcatc agagcctggttgctggcctt gctgacggtg 300 gtgtccatcg tggccctggt cagattgcat ttttggtttggttccagtta tcgctggatc 360 gcctcctggg gcggtttggg gctgttcttc gtactggcggcccgcggtgt gctggcacag 420 gtgttgaagt ggttgcgtgc acggggctgg agccaggggcgcatcattct ggtgggtttg 480 aatcagatgg ccgtcgccgt cagtcggcaa ttgaatcactcttcctgggc cggtttgcag 540 gtgattggtt atgtcgatga ccgggccgaa gaccggctggcggtggcgga ttattcgctg 600 ccacgcctgg gcaagttgag cgatctgcct cgtctggtttccagacaagc cgtggatgaa 660 gtctgggtgg cgtttcctgg cgcttcgctg gccgagcgggtacagcacga attgcgccat 720 ttgccggtca gcattcgcct ggtgatcgat tgctttgcctttaaacaaag caaattcctc 780 agtctgaaca cggtggccgg tatcccgacg ctggacgtctcggtgtcgcc gctgcatggc 840 gtcaatcgct atatcaagga aatcgaggac cgcttgctggccttgctgtt gttgttgctg 900 atcagcccgt tgatgctggt cattgcgctt ggcgtgaaactgagttctcc gggcccggtg 960 ttttacaagc aggtcagagt gggctggaac aatcgcaaattcacgatgct gaagtttcgt 1020 tcgatgccgg tcgatgccga ggccaaaacc ggcgcggtctgggccaggcc cggcgaaaac 1080 cgtgcaaccc ggtttggggc cttcctgcgc aaaaccagtctggacgagtt gccgcagttg 1140 atcaatgtgc tcaagggcga catgtcgctg gtcggcccgcgccctgaacg gcccgatttc 1200 gtcgaggtgt tcaaggatca agtacccaat tacatgaaaaaacacatggt caaggcgggc 1260 attaccggtt gggcacaagt caacggctgg cgcggtgataccgacctgaa tcgccgcatc 1320 gaacacgatc tgtattacat ccagcattgg tcggtctggttcgatctgga gattgccttt 1380 cgcaccgtgt tgaccggctt tatcaacaaa aatgcctat1419 24 473 PRT Methylomonas 16a 24 Met Pro Leu Gly Leu Gly Asn Ile PheAsn Gly Leu Phe Lys Gln Tyr 1 5 10 15 Gly His Thr Val Ile Leu Leu LeuArg Val Ile Asp Val Val Met Leu 20 25 30 Leu Gly Ala Ala Trp Leu Ala HisTyr Phe Trp Leu His Asp Ser Val 35 40 45 Ile Asp Gln His Tyr Arg Phe ValIle Ala Leu Gly Ile Leu Gly Ala 50 55 60 Ile Ile Phe Phe Glu Ile Gly GlnVal Tyr Arg Pro Trp Arg Asn Asp 65 70 75 80 Ala Met Arg Gly Glu Ile ProArg Ile Ile Arg Ala Trp Leu Leu Ala 85 90 95 Leu Leu Thr Val Val Ser IleVal Ala Leu Val Arg Leu His Phe Trp 100 105 110 Phe Gly Ser Ser Tyr ArgTrp Ile Ala Ser Trp Gly Gly Leu Gly Leu 115 120 125 Phe Phe Val Leu AlaAla Arg Gly Val Leu Ala Gln Val Leu Lys Trp 130 135 140 Leu Arg Ala ArgGly Trp Ser Gln Gly Arg Ile Ile Leu Val Gly Leu 145 150 155 160 Asn GlnMet ala Val Ala Val Ser Arg Gln Leu Asn His Ser Ser Trp 165 170 175 AlaGly Leu Gln Val Ile Gly Tyr Val Asp Asp Arg Ala Glu Asp Arg 180 185 190Leu Ala Val Ala Asp Tyr Ser Leu Pro Arg Leu Gly Lys Leu Ser Asp 195 200205 Leu Pro Arg Leu Val Ser Arg Gln Ala Val Asp Glu Val Trp Val Ala 210215 220 Phe Pro Gly Ala Ser Leu Ala Glu Arg Val Gln His Glu Leu Arg His225 230 235 240 Leu Pro Val Ser Ile Arg Leu Val Ile Asp Cys Phe Ala PheLys Gln 245 250 255 Ser Lys Phe Leu Ser Leu Asn Thr Val Ala Gly Ile ProThr Leu Asp 260 265 270 Val Ser Val Ser Pro Leu His Gly Val Asn Arg TyrIle Lys Glu Ile 275 280 285 Glu Asp Arg Leu Leu Ala Leu Leu Leu Leu LeuLeu Ile Ser Pro Leu 290 295 300 Met Leu Val Ile Ala Leu Gly Val Lys LeuSer Ser Pro Gly Pro Val 305 310 315 320 Phe Tyr Lys Gln Val Arg Val GlyTrp Asn Asn Arg Lys Phe Thr Met 325 330 335 Leu Lys Phe Arg Ser Met ProVal Asp Ala Glu Ala Lys Thr Gly Ala 340 345 350 Val Trp Ala Arg Pro GlyGlu Asn Arg Ala Thr Arg Phe Gly Ala Phe 355 360 365 Leu Arg Lys Thr SerLeu Asp Glu Leu Pro Gln Leu Ile Asn Val Leu 370 375 380 Lys Gly Asp MetSer Leu Val Gly Pro Arg Pro Glu Arg Pro Asp Phe 385 390 395 400 Val GluVal Phe Lys Asp Gln Val Pro Asn Tyr Met Lys Lys His Met 405 410 415 ValLys Ala Gly Ile Thr Gly Trp Ala Gln Val Asn Gly Trp Arg Gly 420 425 430Asp Thr Asp Leu Asn Arg Arg Ile Glu His Asp Leu Tyr Tyr Ile Gln 435 440445 His Trp Ser Val Trp Phe Asp Leu Glu Ile Ala Phe Arg Thr Val Leu 450455 460 Thr Gly Phe Ile Asn Lys Asn Ala Tyr 465 470 25 1098 DNAMethylomonas 16a 25 atgtttagac taattcccat catgctggtt ttactgttgccaggctgttt cctggcaccg 60 ggtatggata tgcagaccga tggcgacttg acagaaatcgagctgccaac catgaagggc 120 gggcagttgg tcaaggagaa aacccgcatt cagccgatcaccgccgattt gatcatcgag 180 cgtgaagtcg cacggcggca agccgtcaac aatctaccgccgatggacga aacccggacc 240 agttatcgca tcggtccgca ggacaggttg caaatcacggtatgggagca tcccgaactg 300 aacgatcccg gcggcgagaa aatcctgccg gaactggccggcaaggtcgt ggacgataac 360 ggcgatttgt attaccccta tgtcggtacc cttcatgtcggcggcaagac cgtcaccgaa 420 gtgcgcgagg aattgacccg cgaactgtcc aaatacttcaaaaaggtcaa actcgacatt 480 cgtgtgctgt cgttccaggc tcaccgcgtc gcggtggtcggtgaagtcag aaatcccggc 540 atcgtcgcga tgaccgaaac gccgttgacg gtggcagaagccatcagcag ggccggcggc 600 gccacgcaag attccgattt gaacaacgtc gcgctggcccgcggcggccg gttgtacaaa 660 ctggatgtgc aagccttgta tgaaaaaggc ctgaccacgcaaaacctgct gttgcgggat 720 ggcgatgtgc tgaacgtcgg cgatcagaaa gacagcaaggtttatgtgat gggcgaggtc 780 ggccggcagc aggccatcca gatcaacaag ggccggatgagtctggctca ggcgctggcc 840 gaagcctatg gcgtcgattt caacacctcg cgtcccggcgatatttacgt gctgcgcgcc 900 ggcgacatgc agccggagat tttccagctg gacgccgaatcgcccgacgc gatgatcctg 960 gccgagcaat tcccgttgca gccgcacgac acgctattcgtcggtacggc cggggtcacg 1020 caatggtcca gggtgctgaa tcagattctg ccgggttcgtttaccgccat catgtcgcaa 1080 gccgcgatga tggggatg 1098 26 366 PRTMethylomonas 16a 26 Met Phe Arg Leu Ile Pro Ile Met Leu Val Leu Leu LeuPro Gly Cys 1 5 10 15 Phe Leu Ala Pro Gly Met Asp Met Gln Thr Asp GlyAsp Leu Thr Glu 20 25 30 Ile Glu Leu Pro Thr Met Lys Gly Gly Gln Leu ValLys Glu Lys Thr 35 40 45 Arg Ile Gln Pro Ile Thr Ala Asp Leu Ile Ile GluArg Glu Val Ala 50 55 60 Arg Arg Gln Ala Val Asn Asn Leu Pro Pro Met AspGlu Thr Arg Thr 65 70 75 80 Ser Tyr Arg Ile Gly Pro Gln Asp Arg Leu GlnIle Thr Val Trp Glu 85 90 95 His Pro Glu Leu Asn Asp Pro Gly Gly Glu LysIle Leu Pro Glu Leu 100 105 110 Ala Gly Lys Val Val Asp Asp Asn Gly AspLeu Tyr Tyr Pro Tyr Val 115 120 125 Gly Thr Leu His Val Gly Gly Lys ThrVal Thr Glu Val Arg Glu Glu 130 135 140 Leu Thr Arg Glu Leu Ser Lys TyrPhe Lys Lys Val Lys Leu Asp Ile 145 150 155 160 Arg Val Leu Ser Phe GlnAla His Arg Val Ala Val Val Gly Glu Val 165 170 175 Arg Asn Pro Gly IleVal Ala Met Thr Glu Thr Pro Leu Thr Val Ala 180 185 190 Glu Ala Ile SerArg Ala Gly Gly Ala Thr Gln Asp Ser Asp Leu Asn 195 200 205 Asn Val AlaLeu Ala Arg Gly Gly Arg Leu Tyr Lys Leu Asp Val Gln 210 215 220 Ala LeuTyr Glu Lys Gly Leu Thr Thr Gln Asn Leu Leu Leu Arg Asp 225 230 235 240Gly Asp Val Leu Asn Val Gly Asp Gln Lys Asp Ser Lys Val Tyr Val 245 250255 Met Gly Glu Val Gly Arg Gln Gln Ala Ile Gln Ile Asn Lys Gly Arg 260265 270 Met Ser Leu Ala Gln Ala Leu Ala Glu Ala Tyr Gly Val Asp Phe Asn275 280 285 Thr Ser Arg Pro Gly Asp Ile Tyr Val Leu Arg Ala Gly Asp MetGln 290 295 300 Pro Glu Ile Phe Gln Leu Asp Ala Glu Ser Pro Asp Ala MetIle Leu 305 310 315 320 Ala Glu Gln Phe Pro Leu Gln Pro His Asp Thr LeuPhe Val Gly Thr 325 330 335 Ala Gly Val Thr Gln Trp Ser Arg Val Leu AsnGln Ile Leu Pro Gly 340 345 350 Ser Phe Thr Ala Ile Met Ser Gln Ala AlaMet Met Gly Met 355 360 365 27 2337 DNA Methylomonas 16a 27 atgccgcccttgaatcccgt gatgatgcag gagcctggcg tcagcatccg cgattatgtc 60 gatctgttgatcgagggcaa gaagacaata ctgttgacgt tggccatcgt gctgagcgtg 120 acgatgatttatttggtttt ggccccgcgc acttacaagg ccgatgcctt gctgcgtatc 180 gacaaaaataaagccttgtt ggcggccaat ttgcgtagcg agggcaatgg tacgccaacg 240 gaggcggaaaaccccagggc gcaacgggaa gtggaaattt tgcgctcgcg ttcggtgctg 300 ggcaaggtggtggaggattt gaatctagtc gtggaggcgt cgccacgata ctttcccatc 360 atcggcgaaaccctggcccg caagcacgac aaacatgagg gcgtagccgg cgcctggtgg 420 ggattcagccgttgggcctg gggcggggaa aaactgaaaa tcgagcgttt cgaggtgccc 480 gatcgttacctggacaaggc ttttactttg gtggcgctgg aagcagggcg ttttcaatta 540 ttgagccctaagggcgaggt gctggccgaa ggtttgctcg gtgaaacgct gaccgccgac 600 atcggcgaagccagtcccgt cgtcgtcaac gtcgctgatt tgcaggcgca ttacggcacc 660 gagttcgagttgcggcgcaa aacctcgctg gcggccatag aaaccctgca aaaagccttt 720 tcggtcaaggaagtgtccaa ggataccaat attctgagtg tcgaactcaa ggggcgcgat 780 cccgagcaattggccaaatc ggtcaacgac atcgccagta tttacgtcaa cgccacggtg 840 aattgggaatcggcggaagc ctcgcaaaag ctgaatttcc tggagagcca gttgccgctg 900 gtgaaggagaatctggaaaa ggctgagcaa gccttgagcg cttaccggca gcaacatggc 960 gcggtggatatttccgccga agccgaaatc ctgctgaaac aggcctcgga aatggaaacc 1020 ttgagcatacaactcaagca aaagtacgac gagcaaagcc agcgtctgga atcggagcat 1080 ccggacatgatcgccaccaa tgcgcaaatc cgccgggtga gcaataaatt ggcggccttg 1140 gaaaagcgcatcaaggactt gccgaagacg cagcaaaaca tggtcagcct gtcgcgcgat 1200 gtgcaggtcaataccgagct ttacacctcg ttgctgaaca gcgcgcagga gcaacgcatc 1260 gccgcggccggttccctggg taattcgcgc atcgtcgatt tcgcggtggt tccggaaaaa 1320 ccttattggcccaagcccgg tttgctgttg gcgattgccg gtttgctggg catcagtctg 1380 ggttcggcgctgatattcct gagacattcg ttgcagcgcc atgacaatta tccggccttg 1440 ctggaataccaggtcggctt gccgctgttc gccgccattc cgcacagcaa gaaacaaaga 1500 cgcttggcacgcctgctgga tcagggcaag gagcgggata ccgcgattct ggtcagccac 1560 gatccgctggatatttcggt cgaatccttg cgcggcttgc gcactacgct ggaagcgacg 1620 ctggccagcgatgaaagcaa ggtcatcatg gtcagcagtc cggcgccggg catgggtaaa 1680 tccttcatcagcaccaattt ggcggctctg ttggccagca tacgcaagcg ggtgctgatc 1740 atcgacgccgacatgcgcaa cggccgcctg catgaaacct ttgccattgc caagcaaccg 1800 ggcttgtccgatctgctgtc cggcaaggtc agcctgggcg acgtgatcgt cagtttgccg 1860 gagataggcgtggatttgat tcccaggggc gagatggtgc tgaatccggc cgaattgttg 1920 gtgctgggcgatctggccga taccttggag caactgaaga gcttttacaa ccatatcgtc 1980 atcgattcgccgccgatctt gggcgccacc gacgcggcga tcatgggcaa gcattgcgat 2040 gctaccttcctggtggtcaa ggagggccgt tataccgcgc aagagctgga ggtcagtttc 2100 aggcgcttgcagcaagtcgg cgtgaaaccc aacggtttca tcatcaacga catgaaggaa 2160 ggttcgtcctattacccgta ctacggctat gcctatcagc gggatgacat gcgacaaaaa 2220 caaaccacggcttggcaggc gcgctttcaa aacctgaatg actggatggg gcggcaggac 2280 gccgagtatttacccgtcgc cgacgacgcg gaagaacttc acgacagcat cagggcc 2337 28 779 PRTMethylomonas 16a 28 Met Pro Pro Leu Asn Pro Val Met Met Gln Glu Pro GlyVal Ser Ile 1 5 10 15 Arg Asp Tyr Val Asp Leu Leu Ile Glu Gly Lys LysThr Ile Leu Leu 20 25 30 Thr Leu Ala Ile Val Leu Ser Val Thr Met Ile TyrLeu Val Leu Ala 35 40 45 Pro Arg Thr Tyr Lys Ala Asp Ala Leu Leu Arg IleAsp Lys Asn Lys 50 55 60 Ala Leu Leu Ala Ala Asn Leu Arg Ser Glu Gly AsnGly Thr Pro Thr 65 70 75 80 Glu Ala Glu Asn Pro Arg Ala Gln Arg Glu ValGlu Ile Leu Arg Ser 85 90 95 Arg Ser Val Leu Gly Lys Val Val Glu Asp LeuAsn Leu Val Val Glu 100 105 110 Ala Ser Pro Arg Tyr Phe Pro Ile Ile GlyGlu Thr Leu Ala Arg Lys 115 120 125 His Asp Lys His Glu Gly Val Ala GlyAla Trp Trp Gly Phe Ser Arg 130 135 140 Trp Ala Trp Gly Gly Glu Lys LeuLys Ile Glu Arg Phe Glu Val Pro 145 150 155 160 Asp Arg Tyr Leu Asp LysAla Phe Thr Leu Val Ala Leu Glu Ala Gly 165 170 175 Arg Phe Gln Leu LeuSer Pro Lys Gly Glu Val Leu Ala Glu Gly Leu 180 185 190 Leu Gly Glu ThrLeu Thr Ala Asp Ile Gly Glu Ala Ser Pro Val Val 195 200 205 Val Asn ValAla Asp Leu Gln Ala His Tyr Gly Thr Glu Phe Glu Leu 210 215 220 Arg ArgLys Thr Ser Leu Ala Ala Ile Glu Thr Leu Gln Lys Ala Phe 225 230 235 240Ser Val Lys Glu Val Ser Lys Asp Thr Asn Ile Leu Ser Val Glu Leu 245 250255 Lys Gly Arg Asp Pro Glu Gln Leu Ala Lys Ser Val Asn Asp Ile Ala 260265 270 Ser Ile Tyr Val Asn Ala Thr Val Asn Trp Glu Ser Ala Glu Ala Ser275 280 285 Gln Lys Leu Asn Phe Leu Glu Ser Gln Leu Pro Leu Val Lys GluAsn 290 295 300 Leu Glu Lys Ala Glu Gln Ala Leu Ser Ala Tyr Arg Gln GlnHis Gly 305 310 315 320 Ala Val Asp Ile Ser Ala Glu Ala Glu Ile Leu LeuLys Gln Ala Ser 325 330 335 Glu Met Glu Thr Leu Ser Ile Gln Leu Lys GlnLys Tyr Asp Glu Gln 340 345 350 Ser Gln Arg Leu Glu Ser Glu His Pro AspMet Ile Ala Thr Asn Ala 355 360 365 Gln Ile Arg Arg Val Ser Asn Lys LeuAla Ala Leu Glu Lys Arg Ile 370 375 380 Lys Asp Leu Pro Lys Thr Gln GlnAsn Met Val Ser Leu Ser Arg Asp 385 390 395 400 Val Gln Val Asn Thr GluLeu Tyr Thr Ser Leu Leu Asn Ser Ala Gln 405 410 415 Glu Gln Arg Ile AlaAla Ala Gly Ser Leu Gly Asn Ser Arg Ile Val 420 425 430 Asp Phe Ala ValVal Pro Glu Lys Pro Tyr Trp Pro Lys Pro Gly Leu 435 440 445 Leu Leu AlaIle Ala Gly Leu Leu Gly Ile Ser Leu Gly Ser Ala Leu 450 455 460 Ile PheLeu Arg His Ser Leu Gln Arg His Asp Asn Tyr Pro Ala Leu 465 470 475 480Leu Glu Tyr Gln Val Gly Leu Pro Leu Phe Ala Ala Ile Pro His Ser 485 490495 Lys Lys Gln Arg Arg Leu Ala Arg Leu Leu Asp Gln Gly Lys Glu Arg 500505 510 Asp Thr Ala Ile Leu Val Ser His Asp Pro Leu Asp Ile Ser Val Glu515 520 525 Ser Leu Arg Gly Leu Arg Thr Thr Leu Glu Ala Thr Leu Ala SerAsp 530 535 540 Glu Ser Lys Val Ile Met Val Ser Ser Pro Ala Pro Gly MetGly Lys 545 550 555 560 Ser Phe Ile Ser Thr Asn Leu Ala Ala Leu Leu AlaSer Ile Arg Lys 565 570 575 Arg Val Leu Ile Ile Asp Ala Asp Met Arg AsnGly Arg Leu His Glu 580 585 590 Thr Phe Ala Ile Ala Lys Gln Pro Gly LeuSer Asp Leu Leu Ser Gly 595 600 605 Lys Val Ser Leu Gly Asp Val Ile ValSer Leu Pro Glu Ile Gly Val 610 615 620 Asp Leu Ile Pro Arg Gly Glu MetVal Leu Asn Pro Ala Glu Leu Leu 625 630 635 640 Val Leu Gly Asp Leu AlaAsp Thr Leu Glu Gln Leu Lys Ser Phe Tyr 645 650 655 Asn His Ile Val IleAsp Ser Pro Pro Ile Leu Gly Ala Thr Asp Ala 660 665 670 Ala Ile Met GlyLys His Cys Asp Ala Thr Phe Leu Val Val Lys Glu 675 680 685 Gly Arg TyrThr Ala Gln Glu Leu Glu Val Ser Phe Arg Arg Leu Gln 690 695 700 Gln ValGly Val Lys Pro Asn Gly Phe Ile Ile Asn Asp Met Lys Glu 705 710 715 720Gly Ser Ser Tyr Tyr Pro Tyr Tyr Gly Tyr Ala Tyr Gln Arg Asp Asp 725 730735 Met Arg Gln Lys Gln Thr Thr Ala Trp Gln Ala Arg Phe Gln Asn Leu 740745 750 Asn Asp Trp Met Gly Arg Gln Asp Ala Glu Tyr Leu Pro Val Ala Asp755 760 765 Asp Ala Glu Glu Leu His Asp Ser Ile Arg Ala 770 775 29 1416DNA Methylomonas 16a 29 atgttgggca aagggcattc ggacaaggct aatttaaaggaaggtttcat gctggattgg 60 ttgaggcaaa agaacttgtt gggtgacgcc tgttgggcgctggcgggaca gttattgtcg 120 gcactggctt tgcttgcggg cacgcgcatc ctgaccgaattggtgacgcc ggcggttttc 180 gggcacgtgg cgttgctgaa tggcttcgtc gcgctgggggtggcggtgtt tgcctatccc 240 ttcatctgcg ccgggatgcg tttcaccaat gaatgccgaaatttccgcga gcgggcggca 300 ttgcatggat tggtgtttgc gctgacgacg cgatcgacggcattggccat taccttgctg 360 ctgctgggcg gcgcgctgta ttgctatttt gtcggtagtgaaatcggctt gttcgtgttg 420 accggattgc tgttagccgt caccgttcgc cgcgagttgggcattcagct gatgataggc 480 gaacgcaagc aacgcggcgc cgcgctttgg caaaccagcgacagcatcct gcggccggtg 540 atggcgattt ggctggtatg gggtttgggg caaagtccggaagcggtgtt gttgggctat 600 gtctgtgcca gcgtgctggc caatacgctg tggacgatcgtaagcgatgc atggcaaaaa 660 aagcctaccg gcgatcgcgg cttcctgggg cggcaattcgagcgcggcct ttgggcttat 720 gccttgccgt tgatcccgat ggaattgatg ttctggctcaacggcctggg cgaccgttac 780 gtgatcggtt atttcctaac ggcggctgaa gtgggggtgtacgcggccgc ttatacgctg 840 gtcaacgaag ccttcaatcg tagcgcgatg gtgttgttgcgcacgtttca gccggcctat 900 tttcaagcgg tttcccaagg caaaagcaaa gatgcatgttcgctgctatg gctgtggata 960 ggggcggtcg tcgtgatgag tgttctgggc gtgacgctggtctggttgtg caaggactgg 1020 ctggtcgcag gcttgttggc agaaccctat catgcggccggcgcgctgat gccggttatc 1080 gccgcgggca cggccttgca tgccctgggc accgtgatgtcccagccgct gctggcgaga 1140 aaacgcacgc cgatcttgct gcgcgggcgt atctgtggggcgttggcggc gctcatcacg 1200 ctgcctttgc tggtggcgca ttttggcctg ttcggggcggccttggccaa tcccgtatat 1260 ttcggcatcg aagcgctggt gttggccttg ctggccaagccctggcgcaa gctccgcacg 1320 ggacggcagg cgcggatcgt tcaatccgaa gcggcgatgcccgaacccga ctttgacgcc 1380 atcggagtga gagcggcggc gttctccaac gaatcc 141630 472 PRT Methylomonas 16a 30 Met Leu Gly Lys Gly His Ser Asp Lys AlaAsn Leu Lys Glu Gly Phe 1 5 10 15 Met Leu Asp Trp Leu Arg Gln Lys AsnLeu Leu Gly Asp Ala Cys Trp 20 25 30 Ala Leu Ala Gly Gln Leu Leu Ser AlaLeu Ala Leu Leu Ala Gly Thr 35 40 45 Arg Ile Leu Thr Glu Leu Val Thr ProAla Val Phe Gly His Val Ala 50 55 60 Leu Leu Asn Gly Phe Val Ala Leu GlyVal Ala Val Phe Ala Tyr Pro 65 70 75 80 Phe Ile Cys Ala Gly Met Arg PheThr Asn Glu Cys Arg Asn Phe Arg 85 90 95 Glu Arg Ala Ala Leu His Gly LeuVal Phe Ala Leu Thr Thr Arg Ser 100 105 110 Thr Ala Leu Ala Ile Thr LeuLeu Leu Leu Gly Gly Ala Leu Tyr Cys 115 120 125 Tyr Phe Val Gly Ser GluIle Gly Leu Phe Val Leu Thr Gly Leu Leu 130 135 140 Leu Ala Val Thr ValArg Arg Glu Leu Gly Ile Gln Leu Met Ile Gly 145 150 155 160 Glu Arg LysGln Arg Gly Ala Ala Leu Trp Gln Thr Ser Asp Ser Ile 165 170 175 Leu ArgPro Val Met ala Ile Trp Leu Val Trp Gly Leu Gly Gln Ser 180 185 190 ProGlu Ala Val Leu Leu Gly Tyr Val Cys Ala Ser Val Leu Ala Asn 195 200 205Thr Leu Trp Thr Ile Val Ser Asp Ala Trp Gln Lys Lys Pro Thr Gly 210 215220 Asp Arg Gly Phe Leu Gly Arg Gln Phe Glu Arg Gly Leu Trp Ala Tyr 225230 235 240 Ala Leu Pro Leu Ile Pro Met Glu Leu Met Phe Trp Leu Asn GlyLeu 245 250 255 Gly Asp Arg Tyr Val Ile Gly Tyr Phe Leu Thr Ala Ala GluVal Gly 260 265 270 Val Tyr Ala Ala Ala Tyr Thr Leu Val Asn Glu Ala PheAsn Arg Ser 275 280 285 Ala Met Val Leu Leu Arg Thr Phe Gln Pro Ala TyrPhe Gln Ala Val 290 295 300 Ser Gln Gly Lys Ser Lys Asp Ala Cys Ser LeuLeu Trp Leu Trp Ile 305 310 315 320 Gly Ala Val Val Val Met Ser Val LeuGly Val Thr Leu Val Trp Leu 325 330 335 Cys Lys Asp Trp Leu Val Ala GlyLeu Leu Ala Glu Pro Tyr His Ala 340 345 350 Ala Gly Ala Leu Met Pro ValIle Ala Ala Gly Thr Ala Leu His Ala 355 360 365 Leu Gly Thr Val Met SerGln Pro Leu Leu Ala Arg Lys Arg Thr Pro 370 375 380 Ile Leu Leu Arg GlyArg Ile Cys Gly Ala Leu Ala Ala Leu Ile Thr 385 390 395 400 Leu Pro LeuLeu Val Ala His Phe Gly Leu Phe Gly Ala Ala Leu Ala 405 410 415 Asn ProVal Tyr Phe Gly Ile Glu Ala Leu Val Leu Ala Leu Leu Ala 420 425 430 LysPro Trp Arg Lys Leu Arg Thr Gly Arg Gln Ala Arg Ile Val Gln 435 440 445Ser Glu Ala Ala Met Pro Glu Pro Asp Phe Asp Ala Ile Gly Val Arg 450 455460 Ala Ala Ala Phe Ser Asn Glu Ser 465 470 31 816 DNA Methylomonas 16a31 ccgataaaca ggtgtgaacc attgaacagc ttgaccatag tcattttgac gctgaacgag 60gccgccaatc tgccccggtg cctggcggcg attccgcaac gttaccctgt cgtgatcttg 120gattccggga gcagcgatga cacgctgtcg atcgcggaag gccacggctg caagatttat 180caaaatcctt ggcccggctt tgccgagcag cgcaattttg cgttgaatca atgcgatatc 240gagacgccgt gggtgttgtt cgtcgatgcc gacgaaatct acccgcaagt cttttatcag 300catttcgaca gtggaatgct gcaaaccgga gagatcgatg tgctgatggt gccgtccatt 360ttgtttttgc gcggcaaacg cctgcatcat gcgccgggtt atccgatcta tcacccgcgc 420ctggttcggc gggaaacgac ccgcttcgtg cgtaatcata ccggtcacgg cgaggccgtc 480atggatagtt gccgcatcgg ctacaccgat attccctatg atcattactt ttacgacggc 540gagatcatcc agtggatgca taagcatgtc gacaaagccg ctcaggaagt tcggctcaaa 600ccgacccagg gcgcgttgat gacgacccgc gggcgcttga gcgtaatgct ggggcgttca 660tggagccgaa tcctggccag gtttgtttac cactatctgc tgcgcggcgg ctttttggac 720ggcgcggcgg gattggaatt tacgctgatg tttacctggt atgaagccag catctatctg 780caagccaaag ccgctgcaca agcaagggga acagca 816 32 272 PRT Methylomonas 16a32 Pro Ile Asn Arg Cys Glu Pro Leu Asn Ser Leu Thr Ile Val Ile Leu 1 510 15 Thr Leu Asn Glu Ala Ala Asn Leu Pro Arg Cys Leu Ala Ala Ile Pro 2025 30 Gln Arg Tyr Pro Val Val Ile Leu Asp Ser Gly Ser Ser Asp Asp Thr 3540 45 Leu Ser Ile Ala Glu Gly His Gly Cys Lys Ile Tyr Gln Asn Pro Trp 5055 60 Pro Gly Phe Ala Glu Gln Arg Asn Phe Ala Leu Asn Gln Cys Asp Ile 6570 75 80 Glu Thr Pro Trp Val Leu Phe Val Asp Ala Asp Glu Ile Tyr Pro Gln85 90 95 Val Phe Tyr Gln His Phe Asp Ser Gly Met Leu Gln Thr Gly Glu Ile100 105 110 Asp Val Leu Met Val Pro Ser Ile Leu Phe Leu Arg Gly Lys ArgLeu 115 120 125 His His Ala Pro Gly Tyr Pro Ile Tyr His Pro Arg Leu ValArg Arg 130 135 140 Glu Thr Thr Arg Phe Val Arg Asn His Thr Gly His GlyGlu Ala Val 145 150 155 160 Met Asp Ser Cys Arg Ile Gly Tyr Thr Asp IlePro Tyr Asp His Tyr 165 170 175 Phe Tyr Asp Gly Glu Ile Ile Gln Trp MetHis Lys His Val Asp Lys 180 185 190 Ala Ala Gln Glu Val Arg Leu Lys ProThr Gln Gly Ala Leu Met Thr 195 200 205 Thr Arg Gly Arg Leu Ser Val MetLeu Gly Arg Ser Trp Ser Arg Ile 210 215 220 Leu Ala Arg Phe Val Tyr HisTyr Leu Leu Arg Gly Gly Phe Leu Asp 225 230 235 240 Gly Ala Ala Gly LeuGlu Phe Thr Leu Met Phe Thr Trp Tyr Glu Ala 245 250 255 Ser Ile Tyr LeuGln Ala Lys Ala Ala Ala Gln Ala Arg Gly Thr Ala 260 265 270 33 852 DNAMethylomonas 16a 33 atgaaagtgt cattgatatt ggctacgctc ggcagggacctggaactgct ggattttttg 60 aaatccttgc tgtttcagac ctacaagaac ttcgagttgatcgtcatcga ccagaatcaa 120 gacggcaaaa tcgatcggat tgccgagcaa tatagccaatgcctcgatct gaaacacgtc 180 aaggtgaatt tcaccggtaa tgcccgagcc agggatcatggcatcgcctt ggcccagggc 240 gacatcatcg cctttccgga cgatgattgc gtgtatgaaaaggatgtgct ggaaaaagtg 300 gtaggcgaat ttgcatgcca gccaacgttg tcgattctggtagccgggtc ctacgatttt 360 tccgcgaaac acttcagcat aggcgtcaac agccgtaaagcgcgttattt ttcccggttg 420 aacatgatgg gggtggagtt cacgcagttt tttgcgctggcgcgtatcga caggcggcag 480 ttttatttgg accacgattt cggcatcggc tccaaatatgccggggcgga aggcttcgag 540 ttgctgtatc gcctgctgcg cgcgggcggg cgggcgttctacaagccgga tatcaaaatc 600 tatcacgcca acaaggacca ttacacgctg ggtaccgcgcgcatgctgaa atattccacc 660 ggtattggcg cctatatccg caaattcgcc aatcagcatgatccctatat cggctattac 720 atcctgcgca agatgctgat agccccgact ctgaaaatgctgctggcctt gttgacgttc 780 aacccgggaa aactcgccta ttcgttttat aacctggtgggcatatggcg cggatttttt 840 gcctatgggc gc 852 34 284 PRT Methylomonas 16a34 Met Lys Val Ser Leu Ile Leu Ala Thr Leu Gly Arg Asp Leu Glu Leu 1 510 15 Leu Asp Phe Leu Lys Ser Leu Leu Phe Gln Thr Tyr Lys Asn Phe Glu 2025 30 Leu Ile Val Ile Asp Gln Asn Gln Asp Gly Lys Ile Asp Arg Ile Ala 3540 45 Glu Gln Tyr Ser Gln Cys Leu Asp Leu Lys His Val Lys Val Asn Phe 5055 60 Thr Gly Asn Ala Arg Ala Arg Asp His Gly Ile Ala Leu Ala Gln Gly 6570 75 80 Asp Ile Ile Ala Phe Pro Asp Asp Asp Cys Val Tyr Glu Lys Asp Val85 90 95 Leu Glu Lys Val Val Gly Glu Phe Ala Cys Gln Pro Thr Leu Ser Ile100 105 110 Leu Val Ala Gly Ser Tyr Asp Phe Ser Ala Lys His Phe Ser IleGly 115 120 125 Val Asn Ser Arg Lys Ala Arg Tyr Phe Ser Arg Leu Asn MetMet Gly 130 135 140 Val Glu Phe Thr Gln Phe Phe Ala Leu Ala Arg Ile AspArg Arg Gln 145 150 155 160 Phe Tyr Leu Asp His Asp Phe Gly Ile Gly SerLys Tyr Ala Gly Ala 165 170 175 Glu Gly Phe Glu Leu Leu Tyr Arg Leu LeuArg Ala Gly Gly Arg Ala 180 185 190 Phe Tyr Lys Pro Asp Ile Lys Ile TyrHis Ala Asn Lys Asp His Tyr 195 200 205 Thr Leu Gly Thr Ala Arg Met LeuLys Tyr Ser Thr Gly Ile Gly Ala 210 215 220 Tyr Ile Arg Lys Phe Ala AsnGln His Asp Pro Tyr Ile Gly Tyr Tyr 225 230 235 240 Ile Leu Arg Lys MetLeu Ile Ala Pro Thr Leu Lys Met Leu Leu Ala 245 250 255 Leu Leu Thr PheAsn Pro Gly Lys Leu Ala Tyr Ser Phe Tyr Asn Leu 260 265 270 Val Gly IleTrp Arg Gly Phe Phe Ala Tyr Gly Arg 275 280 35 1194 DNA Methylomonas 16a35 atggaactgg gtattgtgac gacacatgta ccgccggcca agggctacgg tggcgtctcg 60gtgacttgcg gcgtcttgac cagggcgtgg gcggaaatgg ggctagagat ggcgctggtt 120tcgtcggatg aatccatcga tgggtgcttg aaaccggcgg acgtcaagct gggcgcaagc 180gtggatgtcg atttgtaccg ctgttatggc ttcaggcgct gggggttcgg cttgggagcg 240atacccagcc tgctgcgcct gtgctggcaa gccccgctcg tgtatatcca tggcgtcgcc 300acctggccgt cgaccttggc ggcgcttttt tgctgcctgc tgcgcaagcc gttcatggtg 360gcggtgcatg gcggcctgat gcctgagcat gtggcactga tcaagcggaa aaaacggcat 420aaatggtggt attacaaact gctgactttt ccgaccttgc gccgcgcgat tgccgtgcat 480tgcaccagtg ataccgaggt tgagggcgtg cgtgacgtac tgggcgaaaa cgcgcgggtg 540ttgctggtgc ccaacggcat cgacagccgg ggtgtcgagg aggcccctta tccggcaggc 600gaaggcatgc aactgtgttt tttgggtcac gtgcagcagg aaaagggcat caacgctttc 660atccgggcct ggctcgaggt ccggcggccg ggcgatcgtc tggtcgtcgc cggccgtagc 720gtggacgggg attattttgc cgagttttgt tccctggtcg aacgggcaaa cggcgcgatc 780cgctattgcg gctatctgca gcgtgacgac gtgatggcct tgctggcgca aagtcatttt 840ctggtattgc cgtccggttt ggagcaggtc ggcggcatgc gggagaattt cggtaacgtg 900gtggcggaag ccctggcggc gggacggccg gtgctggttg tcaggggctt ggcctgggat 960catttgccgg cattgaatgc gggcttggtt tttgacaggg acgaggccgc cgtccaagcc 1020gtgctacgcc gggctcaggc gctcgatcaa gccgactggc tgcgcatgtc gcaagcgggc 1080cggcgccatg ttcaacagca gctcgatccg gtcaaactgg cggagcgcgt ctggcaagca 1140atgacggcgg cggtaccggt tgacgaggcc aaggtgttgg ccgaggagcc gaaa 1194 36 398PRT Methylomonas 16a 36 Met Glu Leu Gly Ile Val Thr Thr His Val Pro ProAla Lys Gly Tyr 1 5 10 15 Gly Gly Val Ser Val Thr Cys Gly Val Leu ThrArg Ala Trp Ala Glu 20 25 30 Met Gly Leu Glu Met ala Leu Val Ser Ser AspGlu Ser Ile Asp Gly 35 40 45 Cys Leu Lys Pro Ala Asp Val Lys Leu Gly AlaSer Val Asp Val Asp 50 55 60 Leu Tyr Arg Cys Tyr Gly Phe Arg Arg Trp GlyPhe Gly Leu Gly Ala 65 70 75 80 Ile Pro Ser Leu Leu Arg Leu Cys Trp GlnAla Pro Leu Val Tyr Ile 85 90 95 His Gly Val Ala Thr Trp Pro Ser Thr LeuAla Ala Leu Phe Cys Cys 100 105 110 Leu Leu Arg Lys Pro Phe Met Val AlaVal His Gly Gly Leu Met Pro 115 120 125 Glu His Val Ala Leu Ile Lys ArgLys Lys Arg His Lys Trp Trp Tyr 130 135 140 Tyr Lys Leu Leu Thr Phe ProThr Leu Arg Arg Ala Ile Ala Val His 145 150 155 160 Cys Thr Ser Asp ThrGlu Val Glu Gly Val Arg Asp Val Leu Gly Glu 165 170 175 Asn Ala Arg ValLeu Leu Val Pro Asn Gly Ile Asp Ser Arg Gly Val 180 185 190 Glu Glu AlaPro Tyr Pro Ala Gly Glu Gly Met Gln Leu Cys Phe Leu 195 200 205 Gly HisVal Gln Gln Glu Lys Gly Ile Asn Ala Phe Ile Arg Ala Trp 210 215 220 LeuGlu Val Arg Arg Pro Gly Asp Arg Leu Val Val Ala Gly Arg Ser 225 230 235240 Val Asp Gly Asp Tyr Phe Ala Glu Phe Cys Ser Leu Val Glu Arg Ala 245250 255 Asn Gly Ala Ile Arg Tyr Cys Gly Tyr Leu Gln Arg Asp Asp Val Met260 265 270 Ala Leu Leu Ala Gln Ser His Phe Leu Val Leu Pro Ser Gly LeuGlu 275 280 285 Gln Val Gly Gly Met Arg Glu Asn Phe Gly Asn Val Val AlaGlu Ala 290 295 300 Leu Ala Ala Gly Arg Pro Val Leu Val Val Arg Gly LeuAla Trp Asp 305 310 315 320 His Leu Pro Ala Leu Asn Ala Gly Leu Val PheAsp Arg Asp Glu Ala 325 330 335 Ala Val Gln Ala Val Leu Arg Arg Ala GlnAla Leu Asp Gln Ala Asp 340 345 350 Trp Leu Arg Met Ser Gln Ala Gly ArgArg His Val Gln Gln Gln Leu 355 360 365 Asp Pro Val Lys Leu Ala Glu ArgVal Trp Gln Ala Met Thr Ala Ala 370 375 380 Val Pro Val Asp Glu Ala LysVal Leu Ala Glu Glu Pro Lys 385 390 395 37 951 DNA Methylomonas 16a 37atgacgcata aggttggact cgtcgtaccc accttgaatg cgggcgcatc ctggcagggc 60tggctggagg ccctggcggc gcaaagtcga aggccggatc gtttgttgct gatcgattcc 120tcgtccagcg acgacacggt ggcgctggcc cgtgcgagag gatttgacgc gcatgtgatt 180gccaaggcct cgttcaacca cggcggcact cgtcaatcgg gcgtcgatat gttggtcgac 240atggatctga tcgtatttct gacccaggat gccttgttgg ccgaccccag cgcgatcgaa 300aatctgttgc aggtatttgt caatccgcaa gtggccgcgg cctatggccg gcaattgccg 360catcggaacg ctggccccat cggcgcgcat gcccggatat tcaattaccc ggcgcaaagc 420cagttgcgca ccttgcagga ccgcgaccgc ttcggcatca agaccgtgtt catttccaat 480tcgttcgccg cctacagacg ttgcgccctg atgcaaatcg gcggattccc ggctcacacc 540attatgaacg aagatactta cgttgccggc aagatgctgt tgtccggctg gagcctcgcc 600tattgcgccg acgcgcgggt gtttcattcc cacgattaca gcctgctgga agaattcagg 660cgctatttcg atatcggggt tttccacgcg caaaacccct ggctgcaaca gacctttggc 720ggcgcctcgg gcgaaggcgc gcgttttgtg ctctccgaaa tgcgttactt gtcgaacacg 780gcgccctggc tgatgttttc cgcgttcctg agaacgggat tgaaatgggc ggggtataag 840ctgggcggcc tgcatcgcgg ctggccatta gccctgagca ggcgcctcag cctgcataag 900ggatattggg tggcaactga acgggaatac cctaatatgc ctggatgccg t 951 38 317 PRTMethylomonas 16a 38 Met Thr His Lys Val Gly Leu Val Val Pro Thr Leu AsnAla Gly Ala 1 5 10 15 Ser Trp Gln Gly Trp Leu Glu Ala Leu Ala Ala GlnSer Arg Arg Pro 20 25 30 Asp Arg Leu Leu Leu Ile Asp Ser Ser Ser Ser AspAsp Thr Val Ala 35 40 45 Leu Ala Arg Ala Arg Gly Phe Asp Ala His Val IleAla Lys Ala Ser 50 55 60 Phe Asn His Gly Gly Thr Arg Gln Ser Gly Val AspMet Leu Val Asp 65 70 75 80 Met Asp Leu Ile Val Phe Leu Thr Gln Asp AlaLeu Leu Ala Asp Pro 85 90 95 Ser Ala Ile Glu Asn Leu Leu Gln Val Phe ValAsn Pro Gln Val Ala 100 105 110 Ala Ala Tyr Gly Arg Gln Leu Pro His ArgAsn Ala Gly Pro Ile Gly 115 120 125 Ala His Ala Arg Ile Phe Asn Tyr ProAla Gln Ser Gln Leu Arg Thr 130 135 140 Leu Gln Asp Arg Asp Arg Phe GlyIle Lys Thr Val Phe Ile Ser Asn 145 150 155 160 Ser Phe Ala Ala Tyr ArgArg Cys Ala Leu Met Gln Ile Gly Gly Phe 165 170 175 Pro Ala His Thr IleMet Asn Glu Asp Thr Tyr Val Ala Gly Lys Met 180 185 190 Leu Leu Ser GlyTrp Ser Leu Ala Tyr Cys Ala Asp Ala Arg Val Phe 195 200 205 His Ser HisAsp Tyr Ser Leu Leu Glu Glu Phe Arg Arg Tyr Phe Asp 210 215 220 Ile GlyVal Phe His Ala Gln Asn Pro Trp Leu Gln Gln Thr Phe Gly 225 230 235 240Gly Ala Ser Gly Glu Gly Ala Arg Phe Val Leu Ser Glu Met Arg Tyr 245 250255 Leu Ser Asn Thr Ala Pro Trp Leu Met Phe Ser Ala Phe Leu Arg Thr 260265 270 Gly Leu Lys Trp Ala Gly Tyr Lys Leu Gly Gly Leu His Arg Gly Trp275 280 285 Pro Leu Ala Leu Ser Arg Arg Leu Ser Leu His Lys Gly Tyr TrpVal 290 295 300 Ala Thr Glu Arg Glu Tyr Pro Asn Met Pro Gly Cys Arg 305310 315 39 1170 DNA Methylomonas 16a ORF1 39 atgaagcgat ttttaacgttggcaggtgcg gcttattttt ttgccgcatc ggctgttgca 60 gacctgcgcg ccaccggcgatttgggtgtc gtgatcgagc gcgagaccgg cagtgtgcaa 120 gtcatcaaca ccagcacgcccaagatgctg agccgcatcg aaggcctggg cgatttgtct 180 cacgcttcgg tggtgttctcgcgtgatcag cgctatgcct atgtattcgg tcgcgacggc 240 ggcttgagca aaatcgatctgttgcaggac aaaatcgaaa aacgcgtcgt gcaagccggt 300 aacagcatag gcggggcgatttcccaggat ggcaaagtca tcgccgtatc caactatacg 360 ccgggcggcg tcaagctgttcgatgccgag accttggagc agttggccga gattccggcc 420 gtttacggcg acgacaaccagttatccaaa gtggtcggct tggtcgatgc accgggcggt 480 cgtttcgttt gcagcctgttcgaaggtaac gagatttggc tgatagacgc caagaatcca 540 cgccagccgg tcgtcaagaaattcaaggac atcggcaagc ggccttatga tgccttgctg 600 acgccggatg gccatttctacgcggccgga ctgttcggcg aaaaaggcct ggctttgctg 660 gatttatggc agccggagctaggcgtcaaa cacatcctgg aagactacgg caaggacgac 720 gagcaattgc cggtttacaaaatgccgcat ctggaaggct ggacggtagc cggtgatctg 780 ctgttcgtgc cggccatcggcctgcatgag gtgttggtga tcgataaaca cgattgggag 840 ctggtcaaac gcattccggtcgtcggacaa cccgtgttcg tgatgtcccg accggatggt 900 cgccaggtgt gggtgaatttcgcctttccg gacaatcaaa ccgtacaggt catagacgtc 960 aaggatttca atatcgtcaagaccctgcaa ccgggtaagg ccgtgctgca catggagttc 1020 agcccgcgcg gcgaagccgtctggatggcg gtgcgcgacg aggacagggt aatggtttac 1080 gacacggaca gtttcaacgaaaccgcccgt ctaccggcgc aaaagcccag cggcatcttt 1140 ttcagtaatc gcgccaatcagttggggctg 1170 40 390 PRT Methylomonas 16a NirF 40 Met Lys Arg Phe LeuThr Leu Ala Gly Ala Ala Tyr Phe Phe Ala Ala 1 5 10 15 Ser Ala Val AlaAsp Leu Arg Ala Thr Gly Asp Leu Gly Val Val Ile 20 25 30 Glu Arg Glu ThrGly Ser Val Gln Val Ile Asn Thr Ser Thr Pro Lys 35 40 45 Met Leu Ser ArgIle Glu Gly Leu Gly Asp Leu Ser His Ala Ser Val 50 55 60 Val Phe Ser ArgAsp Gln Arg Tyr Ala Tyr Val Phe Gly Arg Asp Gly 65 70 75 80 Gly Leu SerLys Ile Asp Leu Leu Gln Asp Lys Ile Glu Lys Arg Val 85 90 95 Val Gln AlaGly Asn Ser Ile Gly Gly Ala Ile Ser Gln Asp Gly Lys 100 105 110 Val IleAla Val Ser Asn Tyr Thr Pro Gly Gly Val Lys Leu Phe Asp 115 120 125 AlaGlu Thr Leu Glu Gln Leu Ala Glu Ile Pro Ala Val Tyr Gly Asp 130 135 140Asp Asn Gln Leu Ser Lys Val Val Gly Leu Val Asp Ala Pro Gly Gly 145 150155 160 Arg Phe Val Cys Ser Leu Phe Glu Gly Asn Glu Ile Trp Leu Ile Asp165 170 175 Ala Lys Asn Pro Arg Gln Pro Val Val Lys Lys Phe Lys Asp IleGly 180 185 190 Lys Arg Pro Tyr Asp Ala Leu Leu Thr Pro Asp Gly His PheTyr Ala 195 200 205 Ala Gly Leu Phe Gly Glu Lys Gly Leu Ala Leu Leu AspLeu Trp Gln 210 215 220 Pro Glu Leu Gly Val Lys His Ile Leu Glu Asp TyrGly Lys Asp Asp 225 230 235 240 Glu Gln Leu Pro Val Tyr Lys Met Pro HisLeu Glu Gly Trp Thr Val 245 250 255 Ala Gly Asp Leu Leu Phe Val Pro AlaIle Gly Leu His Glu Val Leu 260 265 270 Val Ile Asp Lys His Asp Trp GluLeu Val Lys Arg Ile Pro Val Val 275 280 285 Gly Gln Pro Val Phe Val MetSer Arg Pro Asp Gly Arg Gln Val Trp 290 295 300 Val Asn Phe Ala Phe ProAsp Asn Gln Thr Val Gln Val Ile Asp Val 305 310 315 320 Lys Asp Phe AsnIle Val Lys Thr Leu Gln Pro Gly Lys Ala Val Leu 325 330 335 His Met GluPhe Ser Pro Arg Gly Glu Ala Val Trp Met ala Val Arg 340 345 350 Asp GluAsp Arg Val Met Val Tyr Asp Thr Asp Ser Phe Asn Glu Thr 355 360 365 AlaArg Leu Pro Ala Gln Lys Pro Ser Gly Ile Phe Phe Ser Asn Arg 370 375 380Ala Asn Gln Leu Gly Leu 385 390 41 453 DNA Methylomonas 16a ORF2 41atgctggcat ccttgcacaa gcatttgctg aacgattatc agcaggattt tccgctgagc 60ccgacaccgt ttctggatat cgccgagcag cttggcgtca cggaaggcga agtgctggcg 120gcgtttcagg tgttggccga gcagcaaatg atcagccgca tcggccccgt gatcgcgccg 180aacgccatcg gcaatagcgc cttggtggcg atggcggtgc cggagcagga tttggcccgt 240gtcgccgcct tggtgagcgc ctatccggaa gtcaatcata actatgagcg ggaaaaccgc 300ttcaatttgt ggtttgtgct gatcgcctcc gatcatactc acttgcagcg ggtgattgcc 360gatatcgaga ctcaaaccgg ttatcaagcc atgctgttgc cgatgctggc cgattatttc 420atcaacctgg gttttgaact caatctgaac gac 453 42 151 PRT Methylomonas 16aNirD 42 Met Leu Ala Ser Leu His Lys His Leu Leu Asn Asp Tyr Gln Gln Asp1 5 10 15 Phe Pro Leu Ser Pro Thr Pro Phe Leu Asp Ile Ala Glu Gln LeuGly 20 25 30 Val Thr Glu Gly Glu Val Leu Ala Ala Phe Gln Val Leu Ala GluGln 35 40 45 Gln Met Ile Ser Arg Ile Gly Pro Val Ile Ala Pro Asn Ala IleGly 50 55 60 Asn Ser Ala Leu Val Ala Met ala Val Pro Glu Gln Asp Leu AlaArg 65 70 75 80 Val Ala Ala Leu Val Ser Ala Tyr Pro Glu Val Asn His AsnTyr Glu 85 90 95 Arg Glu Asn Arg Phe Asn Leu Trp Phe Val Leu Ile Ala SerAsp His 100 105 110 Thr His Leu Gln Arg Val Ile Ala Asp Ile Glu Thr GlnThr Gly Tyr 115 120 125 Gln Ala Met Leu Leu Pro Met Leu Ala Asp Tyr PheIle Asn Leu Gly 130 135 140 Phe Glu Leu Asn Leu Asn Asp 145 150 43 504DNA Methylomonas 16a ORF3 43 atggatgcct tggattatcg cttgattgcc gccgtgcaagcgggcttacc gcttaccgcg 60 cggccctatg ccgccatcgc cgcgaaattg gacatggacgaacaggacgt catcgcccga 120 ctgggacgtc tgaaaacgga aggtttgatc aggcgctggggcgtcgtggt caagcaccgg 180 caactaggtt atcgcgccaa tgcgatgatc gtgatggatattcctgatga tcaagttgcg 240 gaaatgggcc ggcgtgtcag ccagcacagc ttcgtcaatctgtgttatcg ccgaccacgt 300 caaggcgagg tttggccgta taacctttat tgcatgatacacggcaaaaa tcgcgagacg 360 gttttgcagc aatgggccga tctgcaacaa agttgcggcctggaagcctg tcggcacgag 420 attttattca gtcgtcgttg tttcaagcag cgtggggctatttataaagc gcccgtgatt 480 gagccattgg agtttagtca tgga 504 44 168 PRTMethylomonas 16a NirL 44 Met Asp Ala Leu Asp Tyr Arg Leu Ile Ala Ala ValGln Ala Gly Leu 1 5 10 15 Pro Leu Thr Ala Arg Pro Tyr Ala Ala Ile AlaAla Lys Leu Asp Met 20 25 30 Asp Glu Gln Asp Val Ile Ala Arg Leu Gly ArgLeu Lys Thr Glu Gly 35 40 45 Leu Ile Arg Arg Trp Gly Val Val Val Lys HisArg Gln Leu Gly Tyr 50 55 60 Arg Ala Asn Ala Met Ile Val Met Asp Ile ProAsp Asp Gln Val Ala 65 70 75 80 Glu Met Gly Arg Arg Val Ser Gln His SerPhe Val Asn Leu Cys Tyr 85 90 95 Arg Arg Pro Arg Gln Gly Glu Val Trp ProTyr Asn Leu Tyr Cys Met 100 105 110 Ile His Gly Lys Asn Arg Glu Thr ValLeu Gln Gln Trp Ala Asp Leu 115 120 125 Gln Gln Ser Cys Gly Leu Glu AlaCys Arg His Glu Ile Leu Phe Ser 130 135 140 Arg Arg Cys Phe Lys Gln ArgGly Ala Ile Tyr Lys Ala Pro Val Ile 145 150 155 160 Glu Pro Leu Glu PheSer His Gly 165 45 441 DNA Methylomonas 16a ORF4 45 atggatgacatcgacaaagc catcatcaac cgtttgcaac agggcttgcc gatttgcgag 60 tcgccttatagatatgtcgc cgagcagctt ggtgtggccg aggcggaatt gctggagagg 120 ctgcaaaccttgttgaacca gggcgtttta tcgcgctttg ggccgatgta tcacgccgag 180 caaatgggcggcgccttgac cttggcggcg atgaaggtgc caggggagcg tttcgacgaa 240 attgcaggcatcgtcaacgg ctttccggag gtggcgcata actatgcgcg taaccacgcc 300 ttgaacatgtggtttgtgtt ggcgaccgaa aagccggaac aagtgcaggc ggtcatcgat 360 gccatcgaacggcaaactgg cttgacggtc tataacatgc cgaaaatcaa ggaatattac 420 gtgggcttgcaactggaggc c 441 46 147 PRT Methylomonas 16a NirG 46 Met Asp Asp Ile AspLys Ala Ile Ile Asn Arg Leu Gln Gln Gly Leu 1 5 10 15 Pro Ile Cys GluSer Pro Tyr Arg Tyr Val Ala Glu Gln Leu Gly Val 20 25 30 Ala Glu Ala GluLeu Leu Glu Arg Leu Gln Thr Leu Leu Asn Gln Gly 35 40 45 Val Leu Ser ArgPhe Gly Pro Met Tyr His Ala Glu Gln Met Gly Gly 50 55 60 Ala Leu Thr LeuAla Ala Met Lys Val Pro Gly Glu Arg Phe Asp Glu 65 70 75 80 Ile Ala GlyIle Val Asn Gly Phe Pro Glu Val Ala His Asn Tyr Ala 85 90 95 Arg Asn HisAla Leu Asn Met Trp Phe Val Leu Ala Thr Glu Lys Pro 100 105 110 Glu GlnVal Gln Ala Val Ile Asp Ala Ile Glu Arg Gln Thr Gly Leu 115 120 125 ThrVal Tyr Asn Met Pro Lys Ile Lys Glu Tyr Tyr Val Gly Leu Gln 130 135 140Leu Glu Ala 145 47 498 DNA Methylomonas 16a ORF5 47 atggactccgagccagtcaa aataatgatc gacactatcg accgtcaaat catccaggcc 60 acccaggccggcttgccgct ggtcgcggaa ccttatcagg ccgtcgccga gcaattgggc 120 atcacggctcaagaattgat gctgcgcatg gccgatatgc tggaagctgg catcattcgg 180 cggattgcggcggtgccgaa tcattacaaa ctgggttatc gtcataacgg catgacggtc 240 tgggatgtcgatgaccggca tgtcgacagc ctggggcagc gcgtcgccga attgccgttc 300 gtcagtcattgctaccaacg gcctcgccat ttgccggatt ggccgtataa cctgttcgcg 360 atggtgcatggcaagacgga acaagacgcc gaaaaacaaa ttgccgtgat cgccgaattg 420 ttgggcgaggattgctaccg gcacgcggtg ctgtacagca ccaagatttt gaagaaaacc 480 ggcttgaggattgcgggg 498 48 166 PRT Methylomonas 16a NirH 48 Met Asp Ser Glu Pro ValLys Ile Met Ile Asp Thr Ile Asp Arg Gln 1 5 10 15 Ile Ile Gln Ala ThrGln Ala Gly Leu Pro Leu Val Ala Glu Pro Tyr 20 25 30 Gln Ala Val Ala GluGln Leu Gly Ile Thr Ala Gln Glu Leu Met Leu 35 40 45 Arg Met ala Asp MetLeu Glu Ala Gly Ile Ile Arg Arg Ile Ala Ala 50 55 60 Val Pro Asn His TyrLys Leu Gly Tyr Arg His Asn Gly Met Thr Val 65 70 75 80 Trp Asp Val AspAsp Arg His Val Asp Ser Leu Gly Gln Arg Val Ala 85 90 95 Glu Leu Pro PheVal Ser His Cys Tyr Gln Arg Pro Arg His Leu Pro 100 105 110 Asp Trp ProTyr Asn Leu Phe Ala Met Val His Gly Lys Thr Glu Gln 115 120 125 Asp AlaGlu Lys Gln Ile Ala Val Ile Ala Glu Leu Leu Gly Glu Asp 130 135 140 CysTyr Arg His Ala Val Leu Tyr Ser Thr Lys Ile Leu Lys Lys Thr 145 150 155160 Gly Leu Arg Ile Ala Gly 165 49 1137 DNA Methylomonas 16a ORF6 49atgtttcgtc tgagtcaata catgcgcgag ctcgtgcatt caacgccgtt gggcaagccg 60cgcaaaccgt ccggcccggt ggtaatctgg aatctgatcc gtcgctgcaa cctgacttgc 120aagcattgct ataccacgtc cgccgacatc gattttccgg gtgaactgac gacgccggaa 180atttatgcgg tgatggacga tttgaaagcc ttcaaggtgc cggtattgat tctgtccggc 240ggagagccgt tgctgcatcc ggatattttt ccgatttcgc aacgcgccag cgacatgggc 300ttttacgtgg ccttgtccag caacggcacg ctgatcgaca aaaacaatat cgagcaaatc 360gccgcgatcg attatcaata tattggcgtc agtctggacg gcatgcgcga ggcgcacgac 420aagttccgcc agaagcaagg ctctttcgac gcctcgctgg ccggcatccg tttatgccgc 480gagcatggca tcaaggccgg cgtgcgcttc acgttgacgc gggacaacgc tcacgatttc 540gatgccttgc tgcagttgat ggacgaggag gacatcgaca aattctatct gtcgcatctg 600aattacggcg gccgcggcaa taaaaaccgg aaagacgatg ccgagtttca gttgacccgc 660aaggtcatgg acgccttgtt cgaaaaggcg ctgagctggg aacagcaagg cctacaccgc 720gaagtggtca ccggcaacaa cgatgccgat gccgtatatt tcctgcattg ggtcaaacgc 780cgctttcccg agcgcgccga gcatatccag gccaagttgc agcaatgggg cggcaatgct 840tccggcgtca acgtagccaa tatcgataat ctgggtaacg tgcatcccga taccttttgg 900tggcattaca acttgggcag tgtccgccag cggccgtttt ccgagatatg gcaggatgtg 960tccgacccat tgatggccgg gctgaaggcc tcgccgcgcc cgctgaaagg ccgctgcggc 1020acctgtcatt atcaaagcat ttgcaacggc aatacccgcg tccgcgccca acaactgacc 1080ggcgattttt gggctgaaga tccaggctgc tacctggatg acgaggaagt tttcagc 1137 50379 PRT Methylomonas 16a Nir J 50 Met Phe Arg Leu Ser Gln Tyr Met ArgGlu Leu Val His Ser Thr Pro 1 5 10 15 Leu Gly Lys Pro Arg Lys Pro SerGly Pro Val Val Ile Trp Asn Leu 20 25 30 Ile Arg Arg Cys Asn Leu Thr CysLys His Cys Tyr Thr Thr Ser Ala 35 40 45 Asp Ile Asp Phe Pro Gly Glu LeuThr Thr Pro Glu Ile Tyr Ala Val 50 55 60 Met Asp Asp Leu Lys Ala Phe LysVal Pro Val Leu Ile Leu Ser Gly 65 70 75 80 Gly Glu Pro Leu Leu His ProAsp Ile Phe Pro Ile Ser Gln Arg Ala 85 90 95 Ser Asp Met Gly Phe Tyr ValAla Leu Ser Ser Asn Gly Thr Leu Ile 100 105 110 Asp Lys Asn Asn Ile GluGln Ile Ala Ala Ile Asp Tyr Gln Tyr Ile 115 120 125 Gly Val Ser Leu AspGly Met Arg Glu Ala His Asp Lys Phe Arg Gln 130 135 140 Lys Gln Gly SerPhe Asp Ala Ser Leu Ala Gly Ile Arg Leu Cys Arg 145 150 155 160 Glu HisGly Ile Lys Ala Gly Val Arg Phe Thr Leu Thr Arg Asp Asn 165 170 175 AlaHis Asp Phe Asp Ala Leu Leu Gln Leu Met Asp Glu Glu Asp Ile 180 185 190Asp Lys Phe Tyr Leu Ser His Leu Asn Tyr Gly Gly Arg Gly Asn Lys 195 200205 Asn Arg Lys Asp Asp Ala Glu Phe Gln Leu Thr Arg Lys Val Met Asp 210215 220 Ala Leu Phe Glu Lys Ala Leu Ser Trp Glu Gln Gln Gly Leu His Arg225 230 235 240 Glu Val Val Thr Gly Asn Asn Asp Ala Asp Ala Val Tyr PheLeu His 245 250 255 Trp Val Lys Arg Arg Phe Pro Glu Arg Ala Glu His IleGln Ala Lys 260 265 270 Leu Gln Gln Trp Gly Gly Asn Ala Ser Gly Val AsnVal Ala Asn Ile 275 280 285 Asp Asn Leu Gly Asn Val His Pro Asp Thr PheTrp Trp His Tyr Asn 290 295 300 Leu Gly Ser Val Arg Gln Arg Pro Phe SerGlu Ile Trp Gln Asp Val 305 310 315 320 Ser Asp Pro Leu Met ala Gly LeuLys Ala Ser Pro Arg Pro Leu Lys 325 330 335 Gly Arg Cys Gly Thr Cys HisTyr Gln Ser Ile Cys Asn Gly Asn Thr 340 345 350 Arg Val Arg Ala Gln GlnLeu Thr Gly Asp Phe Trp Ala Glu Asp Pro 355 360 365 Gly Cys Tyr Leu AspAsp Glu Glu Val Phe Ser 370 375 51 2760 DNA Methylomonas 16a ORF7 51atgtctaaaa ctgccatcaa gacgacttgc ccttattgcg gcgtcggctg cggtatcgaa 60gccagggtgc tcgatgccga aaaccatgtc gtcaatattg ccggcgatcc acagcatcag 120tccaatttcg gccgactgtg ctccaagggc gcggcgctgg gtgataccgt cggtctggaa 180ggccgccttt tatacccgga aatcgatggc cggcgcgtgg attggcccac ggtgctggac 240cggatcgcgg ctaaattcaa tgcgatcatt gccgagcacg gcccggacgc ggtggcgttt 300tatgtgtccg gacagttgtt gaccgaggat tattatgtcg ccaacaaatt gatgaagggc 360ttcatcgggt cggcgaatat cgataccaat tccaggctgt gcatgtcctc ggcggtagtc 420ggttacaagc gtgcgttcgg cgccgatgcg gtgccctgta atttcgagga tctggaacgg 480gcagacttga tcgtgctggt cggttccaac gcggcctggt gccatccgat tgcgtttcag 540cgcatgcgtc aggccaagat agacaatccg gcgctgaaaa tcgtactaat agacccgcgt 600caaaccagca gctgcgatat cgccgatcgg catctggcca tcaagcccgg catggacggc 660ttgttgttca atggcttgct ggtttatctg gccgaaaccg gcgcgttgga tcaggattac 720atcgaacgac actgtgaagg ttttgccgag gccttggcga cggctcgagc gagcgccgcc 780gattttaccg ttctggccaa ccgttgcggc gtggcggcgc acgatctggc gcaactgttt 840gcctggtttg ccggtttgga caaggtcgtg accgtttatt cgcaaggcat caaccagtcc 900agttcaggct ctgataaatg caacgccatc atcaattgcc atctggccag cggcaaaatc 960ggcaaaccgg gctgcggacc gttctcgttt accggccagc ccaacgcgat gggcgggcgc 1020gaggtcggtg ggctggcgaa catgctggcc gcgcacatgg atttggaaaa tccagcgcat 1080gtcgatagag tcgcgcggtt ttggcaaacc gacagcgtcg cccgcaaacc aggcctgaaa 1140gcggtagaaa ttttcgacgc catcgccgac ggtcgcatca aggccttatg gatcatggcg 1200accaaccccg tggtatcgat gccggatgcc gacaaggtaa tcgaagcact taagcaatgc 1260gaatttttgc tggtatcgga ttgcatcgcc aacaccgaca ccgtggagct ggcgcatgtc 1320aaactgccgg ccaccggctg gagcgagaag gacggcaccg tcaccaatct ggaacgtcgc 1380atctcgcggc agcggccatt attccagcct tcgggcgagg cgaaaccgga ttggtggatc 1440gtcagccagg ttgctaagcg catggggttt gccggcttcg attatcgaaa cagcgccgaa 1500atcttcaagg aacacgcggc cttgtccggt tttgaaaatg atgcagcgca ggggggcagg 1560gattttgata tttcaggcct ggcaacgctg gatcaggccc agtttgacgc cttagtgccg 1620atacaatggc ctgtcacagg caagactcaa ggcggaacgg cgcgcctgtt cgaagacggt 1680cgttttttta ccgacaccgg caaggccaga ttcattgcac tcgagccgcg ctcgccaatg 1740cacgccccca caccggatta tccgctggtc ttgaataccg gccgcatccg cgatcaatgg 1800cacacgatga cccgcaccgc gctgtccgcg aagctcaatc aacacaagcc ggaaccgttc 1860gtggagattc atccgcagga tgcgttgcgt tgggggctca aagcaaacgc cctggcccgg 1920atcgaaagcc gttggggcgg catgttggcg cgggtcgacg tcagcgaggc tcagcaaccc 1980ggcagcgtgt tcgtgcccat gcactggacc gcccagctca gcagtcatgg ccgagtcggc 2040gccgtggtca accctgtcgt ggaccctttg tccgggcaac cggaaagcaa gcaaaccccg 2100gtgcgcatcg cggcttgggc accttgctgg caagcgatgg tattgacgaa aatgccattg 2160gacatcgacg attgcgaata ccacgtcaaa ataaggggcc atggcttttg gcgctatcat 2220ttggcggatc aatcccagcg gccagacttg ccggcgtggg gccgcggcat tgtcggcagg 2280ggggcggcca aacccaatga ttgcgtggaa tatctcgacc tggccgctgg cgattaccgc 2340tttgccgaga tgcgggatca aacccttcat gcctgcatgt tcattactca taatggggag 2400ttgccggacc ctggctggct ggccagccta ttcggcaaac cgagattgac ccgcaaggaa 2460cgcttcaacc tgctcagcgg cgtgccgccg caaggggaaa tcgatagcgg caaaacgatc 2520tgctcctgct tcaacgtcgg cgaaaaaacc atcgtgcaag ccattcaaac ccgacatttg 2580agctgtgtaa cagatatagg caactgcctg catgcgggaa cgggttgtgg ttcgtgttta 2640ccggaattaa aaagcatttt ggcccacgcc aaaacgatcg atcctgcctc gctgcccatt 2700cagccaactc aaatcccgcc ggcatcggag gggaaggagg aagccttttt ttcaggtcaa 276052 920 PRT Methylomonas 16a NasA 52 Met Ser Lys Thr Ala Ile Lys Thr ThrCys Pro Tyr Cys Gly Val Gly 1 5 10 15 Cys Gly Ile Glu Ala Arg Val LeuAsp Ala Glu Asn His Val Val Asn 20 25 30 Ile Ala Gly Asp Pro Gln His GlnSer Asn Phe Gly Arg Leu Cys Ser 35 40 45 Lys Gly Ala Ala Leu Gly Asp ThrVal Gly Leu Glu Gly Arg Leu Leu 50 55 60 Tyr Pro Glu Ile Asp Gly Arg ArgVal Asp Trp Pro Thr Val Leu Asp 65 70 75 80 Arg Ile Ala Ala Lys Phe AsnAla Ile Ile Ala Glu His Gly Pro Asp 85 90 95 Ala Val Ala Phe Tyr Val SerGly Gln Leu Leu Thr Glu Asp Tyr Tyr 100 105 110 Val Ala Asn Lys Leu MetLys Gly Phe Ile Gly Ser Ala Asn Ile Asp 115 120 125 Thr Asn Ser Arg LeuCys Met Ser Ser Ala Val Val Gly Tyr Lys Arg 130 135 140 Ala Phe Gly AlaAsp Ala Val Pro Cys Asn Phe Glu Asp Leu Glu Arg 145 150 155 160 Ala AspLeu Ile Val Leu Val Gly Ser Asn Ala Ala Trp Cys His Pro 165 170 175 IleAla Phe Gln Arg Met Arg Gln Ala Lys Ile Asp Asn Pro Ala Leu 180 185 190Lys Ile Val Leu Ile Asp Pro Arg Gln Thr Ser Ser Cys Asp Ile Ala 195 200205 Asp Arg His Leu Ala Ile Lys Pro Gly Met Asp Gly Leu Leu Phe Asn 210215 220 Gly Leu Leu Val Tyr Leu Ala Glu Thr Gly Ala Leu Asp Gln Asp Tyr225 230 235 240 Ile Glu Arg His Cys Glu Gly Phe Ala Glu Ala Leu Ala ThrAla Arg 245 250 255 Ala Ser Ala Ala Asp Phe Thr Val Leu Ala Asn Arg CysGly Val Ala 260 265 270 Ala His Asp Leu Ala Gln Leu Phe Ala Trp Phe AlaGly Leu Asp Lys 275 280 285 Val Val Thr Val Tyr Ser Gln Gly Ile Asn GlnSer Ser Ser Gly Ser 290 295 300 Asp Lys Cys Asn Ala Ile Ile Asn Cys HisLeu Ala Ser Gly Lys Ile 305 310 315 320 Gly Lys Pro Gly Cys Gly Pro PheSer Phe Thr Gly Gln Pro Asn Ala 325 330 335 Met Gly Gly Arg Glu Val GlyGly Leu Ala Asn Met Leu Ala Ala His 340 345 350 Met Asp Leu Glu Asn ProAla His Val Asp Arg Val Ala Arg Phe Trp 355 360 365 Gln Thr Asp Ser ValAla Arg Lys Pro Gly Leu Lys Ala Val Glu Ile 370 375 380 Phe Asp Ala IleAla Asp Gly Arg Ile Lys Ala Leu Trp Ile Met ala 385 390 395 400 Thr AsnPro Val Val Ser Met Pro Asp Ala Asp Lys Val Ile Glu Ala 405 410 415 LeuLys Gln Cys Glu Phe Leu Leu Val Ser Asp Cys Ile Ala Asn Thr 420 425 430Asp Thr Val Glu Leu Ala His Val Lys Leu Pro Ala Thr Gly Trp Ser 435 440445 Glu Lys Asp Gly Thr Val Thr Asn Leu Glu Arg Arg Ile Ser Arg Gln 450455 460 Arg Pro Leu Phe Gln Pro Ser Gly Glu Ala Lys Pro Asp Trp Trp Ile465 470 475 480 Val Ser Gln Val Ala Lys Arg Met Gly Phe Ala Gly Phe AspTyr Arg 485 490 495 Asn Ser Ala Glu Ile Phe Lys Glu His Ala Ala Leu SerGly Phe Glu 500 505 510 Asn Asp Ala Ala Gln Gly Gly Arg Asp Phe Asp IleSer Gly Leu Ala 515 520 525 Thr Leu Asp Gln Ala Gln Phe Asp Ala Leu ValPro Ile Gln Trp Pro 530 535 540 Val Thr Gly Lys Thr Gln Gly Gly Thr AlaArg Leu Phe Glu Asp Gly 545 550 555 560 Arg Phe Phe Thr Asp Thr Gly LysAla Arg Phe Ile Ala Leu Glu Pro 565 570 575 Arg Ser Pro Met His Ala ProThr Pro Asp Tyr Pro Leu Val Leu Asn 580 585 590 Thr Gly Arg Ile Arg AspGln Trp His Thr Met Thr Arg Thr Ala Leu 595 600 605 Ser Ala Lys Leu AsnGln His Lys Pro Glu Pro Phe Val Glu Ile His 610 615 620 Pro Gln Asp AlaLeu Arg Trp Gly Leu Lys Ala Asn Ala Leu Ala Arg 625 630 635 640 Ile GluSer Arg Trp Gly Gly Met Leu Ala Arg Val Asp Val Ser Glu 645 650 655 AlaGln Gln Pro Gly Ser Val Phe Val Pro Met His Trp Thr Ala Gln 660 665 670Leu Ser Ser His Gly Arg Val Gly Ala Val Val Asn Pro Val Val Asp 675 680685 Pro Leu Ser Gly Gln Pro Glu Ser Lys Gln Thr Pro Val Arg Ile Ala 690695 700 Ala Trp Ala Pro Cys Trp Gln Ala Met Val Leu Thr Lys Met Pro Leu705 710 715 720 Asp Ile Asp Asp Cys Glu Tyr His Val Lys Ile Arg Gly HisGly Phe 725 730 735 Trp Arg Tyr His Leu Ala Asp Gln Ser Gln Arg Pro AspLeu Pro Ala 740 745 750 Trp Gly Arg Gly Ile Val Gly Arg Gly Ala Ala LysPro Asn Asp Cys 755 760 765 Val Glu Tyr Leu Asp Leu Ala Ala Gly Asp TyrArg Phe Ala Glu Met 770 775 780 Arg Asp Gln Thr Leu His Ala Cys Met PheIle Thr His Asn Gly Glu 785 790 795 800 Leu Pro Asp Pro Gly Trp Leu AlaSer Leu Phe Gly Lys Pro Arg Leu 805 810 815 Thr Arg Lys Glu Arg Phe AsnLeu Leu Ser Gly Val Pro Pro Gln Gly 820 825 830 Glu Ile Asp Ser Gly LysThr Ile Cys Ser Cys Phe Asn Val Gly Glu 835 840 845 Lys Thr Ile Val GlnAla Ile Gln Thr Arg His Leu Ser Cys Val Thr 850 855 860 Asp Ile Gly AsnCys Leu His Ala Gly Thr Gly Cys Gly Ser Cys Leu 865 870 875 880 Pro GluLeu Lys Ser Ile Leu Ala His Ala Lys Thr Ile Asp Pro Ala 885 890 895 SerLeu Pro Ile Gln Pro Thr Gln Ile Pro Pro Ala Ser Glu Gly Lys 900 905 910Glu Glu Ala Phe Phe Ser Gly Gln 915 920 53 642 DNA Methylomonas 16a ORF853 atggcaacga aaccgaacat tcacatcaac ctggaggtcg tcatgactga gcaagtcccg 60cgctgggcgt cggaaacatt ctggaaaaaa accgcgatct gggtcaccgg cggatcgttc 120gtgttgctgg tgatcttgac cttcgactcg ctggcgaaga tttccgctgg cggccccagg 180gtgccggcct tcgacgtcat caacaaagac gtcagttacc gtttcgacaa ggaaaaacaa 240cgctaccaac cagtgatcgg cgacgacgcc cctctgtttg gcaaaaccct gagcgaggaa 300gaagccgaaa aactggtcga cctgggcaag aaaaccgtgc aggccaagaa ctgcatgaac 360tgccataccc tgctcggcaa tggcgcttat tatgcgcccg acttgaccaa ggcctggctg 420gaccagggct ggatcgccaa ggagtcgcgc gagcaaatga tggtcaattt cctgctcgat 480cccgagaaaa atgcccgcac cttcggctcc aaccgcaaga tgccgaatct cgacatcacg 540caacaggagg ccgagggcat cgtcgccttt ttgaaatgga tggcatccat cgacaccaat 600ggttttccgc ataatttcat cgcgctgggc gaagaggaca aa 642 54 214 PRTMethylomonas 16a NorC 54 Met ala Thr Lys Pro Asn Ile His Ile Asn Leu GluVal Val Met Thr 1 5 10 15 Glu Gln Val Pro Arg Trp Ala Ser Glu Thr PheTrp Lys Lys Thr Ala 20 25 30 Ile Trp Val Thr Gly Gly Ser Phe Val Leu LeuVal Ile Leu Thr Phe 35 40 45 Asp Ser Leu Ala Lys Ile Ser Ala Gly Gly ProArg Val Pro Ala Phe 50 55 60 Asp Val Ile Asn Lys Asp Val Ser Tyr Arg PheAsp Lys Glu Lys Gln 65 70 75 80 Arg Tyr Gln Pro Val Ile Gly Asp Asp AlaPro Leu Phe Gly Lys Thr 85 90 95 Leu Ser Glu Glu Glu Ala Glu Lys Leu ValAsp Leu Gly Lys Lys Thr 100 105 110 Val Gln Ala Lys Asn Cys Met Asn CysHis Thr Leu Leu Gly Asn Gly 115 120 125 Ala Tyr Tyr Ala Pro Asp Leu ThrLys Ala Trp Leu Asp Gln Gly Trp 130 135 140 Ile Ala Lys Glu Ser Arg GluGln Met Met Val Asn Phe Leu Leu Asp 145 150 155 160 Pro Glu Lys Asn AlaArg Thr Phe Gly Ser Asn Arg Lys Met Pro Asn 165 170 175 Leu Asp Ile ThrGln Gln Glu Ala Glu Gly Ile Val Ala Phe Leu Lys 180 185 190 Trp Met alaSer Ile Asp Thr Asn Gly Phe Pro His Asn Phe Ile Ala 195 200 205 Leu GlyGlu Glu Asp Lys 210 55 1503 DNA Methylomonas 16a ORF9 55 atgacgctacaagcctatca agaaaaagcc gctgtctgct gggccggttg caagcaacgc 60 cacgccgacttcatggccaa tccgcatttg accggcggcc aaaagctggc ggtgcattac 120 ttcaccgtcgccatggtgct gttcatggcg caattgctgt tcggcctgct ggccggcctg 180 caattcatcttcccgagttt tttatacgaa atcctggatt tcaacgtcaa ccgcatggtg 240 cacatcaatgccatggtggt gtggatgctg tacggctttt tgggctcggt gtactggttt 300 ctggaagacgagagcggcgt cgagatcgtc ggcttgaaat gggggcaact ggcgttttgg 360 gtgctgaccggtgcggtcgc gctggtcgtg ctggtgtatt tgttcatcca gatcggcgcc 420 ggcaacgacacttcgctgtg gctgatcaac gaaggccgcg aatacatcga agccccgcgc 480 tgggccgacatcggcatcgt cgccgtggta ttgaccttct tttacaacgt cgccgccacc 540 ttcgccaaaggcaaatggtc cggcattgcc ggcgtgttga ccctggatct ggtggccctg 600 gccggcttgtatctggccgg catgttctac gtcaccaata tttcggtcga ccaatactgg 660 tggtggtgggtgatccatct atgggtcgaa gcgacctggg aagtgctggt tggctgcatc 720 atggcctggagcctgatgaa gctgctgggc gtgcgccgcc aggtcgtaca aacttggttg 780 tacatcgaagttgctttgat gttcggctcc ggcattcttg gcctgggtca tcactatttc 840 tggatcggcacgccggaata ctggttcagc atcggcggct tcttctcggc gctggaaccg 900 attccgctggtagcaatggt cgtgcattcc atttacgatt ccggcgtgca caagtttaaa 960 aacagcaatcaccccgccct ggcctggatc atcgcccata ctttcggcaa cttcctgggc 1020 gccggcgtttggggattcat gcacacgctg ccgcaaatca acctgtacac ccacggcacg 1080 caatggtcggcctcgcacgg ccacctggcc ttcttcggcg cctatgcgac catcaacatc 1140 gccttcttctacctggcggc gcagcaggcg cgcggcaacg tctggatggg cggtgacttg 1200 atcaacggctggcgctggaa agcggcggcg attttgctaa atctgggcgt gttgggcatg 1260 accgtggcgctattgatcgc cggttacgag caatcgttta tcgaacgcgc cgtcgaaggc 1320 tcgacctgggccggttactt cgccgcgcaa aaccacccgt ggttcatgca agccatggtc 1380 tggcgcatggtattcggctt gatgacggcc gtcggcggcg gcctgttgtt ctgggacttg 1440 ctggaaatcggcaaaggcga acagcggccc gcggcggtga ttgccggtgg aacggttgcg 1500 gaa 1503 56501 PRT Methylomonas 16a NorB 56 Met Thr Leu Gln Ala Tyr Gln Glu Lys AlaAla Val Cys Trp Ala Gly 1 5 10 15 Cys Lys Gln Arg His Ala Asp Phe Metala Asn Pro His Leu Thr Gly 20 25 30 Gly Gln Lys Leu Ala Val His Tyr PheThr Val Ala Met Val Leu Phe 35 40 45 Met ala Gln Leu Leu Phe Gly Leu LeuAla Gly Leu Gln Phe Ile Phe 50 55 60 Pro Ser Phe Leu Tyr Glu Ile Leu AspPhe Asn Val Asn Arg Met Val 65 70 75 80 His Ile Asn Ala Met Val Val TrpMet Leu Tyr Gly Phe Leu Gly Ser 85 90 95 Val Tyr Trp Phe Leu Glu Asp GluSer Gly Val Glu Ile Val Gly Leu 100 105 110 Lys Trp Gly Gln Leu Ala PheTrp Val Leu Thr Gly Ala Val Ala Leu 115 120 125 Val Val Leu Val Tyr LeuPhe Ile Gln Ile Gly Ala Gly Asn Asp Thr 130 135 140 Ser Leu Trp Leu IleAsn Glu Gly Arg Glu Tyr Ile Glu Ala Pro Arg 145 150 155 160 Trp Ala AspIle Gly Ile Val Ala Val Val Leu Thr Phe Phe Tyr Asn 165 170 175 Val AlaAla Thr Phe Ala Lys Gly Lys Trp Ser Gly Ile Ala Gly Val 180 185 190 LeuThr Leu Asp Leu Val Ala Leu Ala Gly Leu Tyr Leu Ala Gly Met 195 200 205Phe Tyr Val Thr Asn Ile Ser Val Asp Gln Tyr Trp Trp Trp Trp Val 210 215220 Ile His Leu Trp Val Glu Ala Thr Trp Glu Val Leu Val Gly Cys Ile 225230 235 240 Met ala Trp Ser Leu Met Lys Leu Leu Gly Val Arg Arg Gln ValVal 245 250 255 Gln Thr Trp Leu Tyr Ile Glu Val Ala Leu Met Phe Gly SerGly Ile 260 265 270 Leu Gly Leu Gly His His Tyr Phe Trp Ile Gly Thr ProGlu Tyr Trp 275 280 285 Phe Ser Ile Gly Gly Phe Phe Ser Ala Leu Glu ProIle Pro Leu Val 290 295 300 Ala Met Val Val His Ser Ile Tyr Asp Ser GlyVal His Lys Phe Lys 305 310 315 320 Asn Ser Asn His Pro Ala Leu Ala TrpIle Ile Ala His Thr Phe Gly 325 330 335 Asn Phe Leu Gly Ala Gly Val TrpGly Phe Met His Thr Leu Pro Gln 340 345 350 Ile Asn Leu Tyr Thr His GlyThr Gln Trp Ser Ala Ser His Gly His 355 360 365 Leu Ala Phe Phe Gly AlaTyr Ala Thr Ile Asn Ile Ala Phe Phe Tyr 370 375 380 Leu Ala Ala Gln GlnAla Arg Gly Asn Val Trp Met Gly Gly Asp Leu 385 390 395 400 Ile Asn GlyTrp Arg Trp Lys Ala Ala Ala Ile Leu Leu Asn Leu Gly 405 410 415 Val LeuGly Met Thr Val Ala Leu Leu Ile Ala Gly Tyr Glu Gln Ser 420 425 430 PheIle Glu Arg Ala Val Glu Gly Ser Thr Trp Ala Gly Tyr Phe Ala 435 440 445Ala Gln Asn His Pro Trp Phe Met Gln Ala Met Val Trp Arg Met Val 450 455460 Phe Gly Leu Met Thr Ala Val Gly Gly Gly Leu Leu Phe Trp Asp Leu 465470 475 480 Leu Glu Ile Gly Lys Gly Glu Gln Arg Pro Ala Ala Val Ile AlaGly 485 490 495 Gly Thr Val Ala Glu 500 57 2253 DNA Methylomonas 16aORF10 57 atgacaaaaa ctcctgattt gtctccttgg tggctgcgca cggtgctgatcgtgatggtg 60 ctgggctttg caggcctgat cgtcatcaca tcgctggcct atcgtaatgctccgccgatt 120 ccggcccaaa tcgttgatgc acaaggtgtt cgcctgtttt ccggtgacgaaatcaaagaa 180 ggccaggcta tctttctcaa atacgggttg atgaacaacg gcagtatctggggtcatggc 240 gcatacttgg ggccagatta ttcggccgag gcattgcacc gaatcggcgagaacaccgcc 300 actatcattg cccagcagca ataccaacag ccactttcct cactcacgcccggccaattg 360 gccgccgtgt atgcacaaac cgcagtcgag ctaaagacca atcattatgatgccgccagc 420 gcaacgttgc gtctgaccgt gccggagaca tccgcctatc gtaagcagatcgcttattgg 480 acggattatt tcctgaatcc tgaacggaat ggcggactca aacgtggattgatcagcgat 540 ccgactgaac tgcgccagtt taccgccttc atcacatgga ctgcctgggcctcggtggcc 600 aaccgccccg gcgagaacta ctcctacacc aacaattttc catacgaccccagcgtcggg 660 aatatgcccg ttcccggtgc gctgttatgg agtgcgttga gccttatcgtgctgctggcc 720 ggtattggaa tcgtacttct gatgtttgga aaattcgatt atcttggctggattagcaca 780 ggacatcatg tacatcctca tctgttgcct gggcaagcca gtgccggtcaactagcactg 840 gtgaaatttt tcgtggtggt ggcgctgctg tttcttgctc agaccttggtgggcggtgca 900 acggcgcact atcgcgccga tccaggcagt ttttacggcc ttgagctggagaagctattt 960 cccagcaatc tgatgcgcac ctggcatcta caaaccgcgg ttttctggattgccaccgct 1020 tttgtcgccg cagccttgtt tctcggtcgt tcactgcgca atgatgaacctcgctggttc 1080 gcgggctggg ttcatctgct gttcggtgct ttcgccgtgg tcataggcggtagcctgtta 1140 ggcgagtggg cggggatttc acaaatgctg gatcaatggt ggttctggcttggcaaccag 1200 ggttgggaat acctggagct cggccgtctg tggcagtacc tgcttatcgccggtctgctg 1260 gcgtggttta cgcttttatt taagttgcta cagcctgata ccctgaacgactcagaagcg 1320 aaacctttag tcaggctgtt cctgctagct tccttggcga ttccgctgttctacatcccg 1380 gcactcttct tcggcgcaaa gaccaacttc acagtggtcg atacctggcgcttctggatc 1440 attcatttat gggtcgaagg tttctttgaa ttctttgcca ccacgctggtggcgctgctg 1500 ttttatcaac tgggtcttac ccagcgcaac gttgcgcttc gagtgatttacctcgacgcc 1560 atcctctatt tcgtcggcgg cctgattggt accggccatc actggtattttaccggccag 1620 agcagcgtca acatggcgct gtcggcaatg gtctcggtac tggaagtagtgcccttgacg 1680 ctgctgactc tggacgcctg ggatttcgtg cgcaccacac gcgctgactgcgacgtctgc 1740 ggcaaaccgg tagccatacc gcataaatgg acgttctatt tcttgatggccgtcggcttc 1800 tggaatttcg tcggtgccgg catcttcggc tttctaatca acctgcctatcgtcagctat 1860 tatgaagtcg gaacccaact gacacccaac catggccatg ccgcgatgatgggggtattc 1920 ggcatgctgg cactggcact gatggtattc gtgttgcgcc agaccagctccgatttgcgc 1980 tgggtcgaca tcgagaaata cgtaagggtc ggattttggg gctccaatgttggcctggct 2040 ctgatgttaa tcatgagctt gttccccagt ggcgtgttgc aagtttgggatgtcgttcag 2100 catggatact ggcatgcgcg cagccttgat tacatcggca gcgaaaggtcgcgcctgatc 2160 gaatggctac gtctgcccgg tgatctggta tttatcctgt ttggcgccataccgttggca 2220 atcgcatcca tcaaaggctg gctggatgtg cat 2253 58 751 PRTMethylomonas 16a NorZ 58 Met Thr Lys Thr Pro Asp Leu Ser Pro Trp Trp LeuArg Thr Val Leu 1 5 10 15 Ile Val Met Val Leu Gly Phe Ala Gly Leu IleVal Ile Thr Ser Leu 20 25 30 Ala Tyr Arg Asn Ala Pro Pro Ile Pro Ala GlnIle Val Asp Ala Gln 35 40 45 Gly Val Arg Leu Phe Ser Gly Asp Glu Ile LysGlu Gly Gln Ala Ile 50 55 60 Phe Leu Lys Tyr Gly Leu Met Asn Asn Gly SerIle Trp Gly His Gly 65 70 75 80 Ala Tyr Leu Gly Pro Asp Tyr Ser Ala GluAla Leu His Arg Ile Gly 85 90 95 Glu Asn Thr Ala Thr Ile Ile Ala Gln GlnGln Tyr Gln Gln Pro Leu 100 105 110 Ser Ser Leu Thr Pro Gly Gln Leu AlaAla Val Tyr Ala Gln Thr Ala 115 120 125 Val Glu Leu Lys Thr Asn His TyrAsp Ala Ala Ser Ala Thr Leu Arg 130 135 140 Leu Thr Val Pro Glu Thr SerAla Tyr Arg Lys Gln Ile Ala Tyr Trp 145 150 155 160 Thr Asp Tyr Phe LeuAsn Pro Glu Arg Asn Gly Gly Leu Lys Arg Gly 165 170 175 Leu Ile Ser AspPro Thr Glu Leu Arg Gln Phe Thr Ala Phe Ile Thr 180 185 190 Trp Thr AlaTrp Ala Ser Val Ala Asn Arg Pro Gly Glu Asn Tyr Ser 195 200 205 Tyr ThrAsn Asn Phe Pro Tyr Asp Pro Ser Val Gly Asn Met Pro Val 210 215 220 ProGly Ala Leu Leu Trp Ser Ala Leu Ser Leu Ile Val Leu Leu Ala 225 230 235240 Gly Ile Gly Ile Val Leu Leu Met Phe Gly Lys Phe Asp Tyr Leu Gly 245250 255 Trp Ile Ser Thr Gly His His Val His Pro His Leu Leu Pro Gly Gln260 265 270 Ala Ser Ala Gly Gln Leu Ala Leu Val Lys Phe Phe Val Val ValAla 275 280 285 Leu Leu Phe Leu Ala Gln Thr Leu Val Gly Gly Ala Thr AlaHis Tyr 290 295 300 Arg Ala Asp Pro Gly Ser Phe Tyr Gly Leu Glu Leu GluLys Leu Phe 305 310 315 320 Pro Ser Asn Leu Met Arg Thr Trp His Leu GlnThr Ala Val Phe Trp 325 330 335 Ile Ala Thr Ala Phe Val Ala Ala Ala LeuPhe Leu Gly Arg Ser Leu 340 345 350 Arg Asn Asp Glu Pro Arg Trp Phe AlaGly Trp Val His Leu Leu Phe 355 360 365 Gly Ala Phe Ala Val Val Ile GlyGly Ser Leu Leu Gly Glu Trp Ala 370 375 380 Gly Ile Ser Gln Met Leu AspGln Trp Trp Phe Trp Leu Gly Asn Gln 385 390 395 400 Gly Trp Glu Tyr LeuGlu Leu Gly Arg Leu Trp Gln Tyr Leu Leu Ile 405 410 415 Ala Gly Leu LeuAla Trp Phe Thr Leu Leu Phe Lys Leu Leu Gln Pro 420 425 430 Asp Thr LeuAsn Asp Ser Glu Ala Lys Pro Leu Val Arg Leu Phe Leu 435 440 445 Leu AlaSer Leu Ala Ile Pro Leu Phe Tyr Ile Pro Ala Leu Phe Phe 450 455 460 GlyAla Lys Thr Asn Phe Thr Val Val Asp Thr Trp Arg Phe Trp Ile 465 470 475480 Ile His Leu Trp Val Glu Gly Phe Phe Glu Phe Phe Ala Thr Thr Leu 485490 495 Val Ala Leu Leu Phe Tyr Gln Leu Gly Leu Thr Gln Arg Asn Val Ala500 505 510 Leu Arg Val Ile Tyr Leu Asp Ala Ile Leu Tyr Phe Val Gly GlyLeu 515 520 525 Ile Gly Thr Gly His His Trp Tyr Phe Thr Gly Gln Ser SerVal Asn 530 535 540 Met ala Leu Ser Ala Met Val Ser Val Leu Glu Val ValPro Leu Thr 545 550 555 560 Leu Leu Thr Leu Asp Ala Trp Asp Phe Val ArgThr Thr Arg Ala Asp 565 570 575 Cys Asp Val Cys Gly Lys Pro Val Ala IlePro His Lys Trp Thr Phe 580 585 590 Tyr Phe Leu Met ala Val Gly Phe TrpAsn Phe Val Gly Ala Gly Ile 595 600 605 Phe Gly Phe Leu Ile Asn Leu ProIle Val Ser Tyr Tyr Glu Val Gly 610 615 620 Thr Gln Leu Thr Pro Asn HisGly His Ala Ala Met Met Gly Val Phe 625 630 635 640 Gly Met Leu Ala LeuAla Leu Met Val Phe Val Leu Arg Gln Thr Ser 645 650 655 Ser Asp Leu ArgTrp Val Asp Ile Glu Lys Tyr Val Arg Val Gly Phe 660 665 670 Trp Gly SerAsn Val Gly Leu Ala Leu Met Leu Ile Met Ser Leu Phe 675 680 685 Pro SerGly Val Leu Gln Val Trp Asp Val Val Gln His Gly Tyr Trp 690 695 700 HisAla Arg Ser Leu Asp Tyr Ile Gly Ser Glu Arg Ser Arg Leu Ile 705 710 715720 Glu Trp Leu Arg Leu Pro Gly Asp Leu Val Phe Ile Leu Phe Gly Ala 725730 735 Ile Pro Leu Ala Ile Ala Ser Ile Lys Gly Trp Leu Asp Val His 740745 750 59 1581 DNA Methylomonas 16a ORF11 59 atgatgaaaa caacaactaaaagacgactg aatcaatccc ttctggcgag tgctatcgcc 60 gcgttactgt cgtccggtgcggtgctggcg aaatccgaca gcccacacga catctacatg 120 gataattgcg ccagctgccacggcgcggat cacggtggct atctggcgcc agccttgaat 180 gccgatacct tgaaaggtcgtagccctacg gcgttgcgta ccatcgtcat ggccggcagc 240 ttcgatacgc tgatgcctcccttctacggc aaactgagcg acgacgagat tcgcggcgtg 300 atcaagcatt tgcaggaaaccccgaaacag ccgaatccgg cctggaccat cgacgacatg 360 aagaagtcct tgaaggtttacgtcaaggat gagagcaccc tgcctggcaa gccgactttc 420 caaatcgata acatcgataatctgatcggc gtggcggcac gcggcaaata cggccgtggc 480 gaaggctcca aagctattttcatcaacagc accaaccatc aaaaagtcgg cgaagtggct 540 accggcaccg ccgcgcatatcatcgacttc aatcctgcca acccgcgctg ggcttacgta 600 aaaaccgaca ccgccgagattttcaaggta gatttgtatt cgatgcaggc ggtccgcagc 660 atcaagacag gttacaacggccccggcatg ggggtatccc gcgacggcaa atacatcatg 720 gccggctcct tcgtgccgcataacgccgta atcctggatg ccgaaaccct ggaaccgttg 780 aaaaccttcg aactggaaggcatcgatccc gacggtaaac atgtttcttc cgactcgggc 840 atgatcatcg gtaccccttatgccgacgtg ttcgcgattg cgctggaaaa tgccggccag 900 gtctggatcg tcgattacaacaaagaaggc ttcccggtca ccaaaatcga gaaagtgggc 960 cgtcacttgc acgacgccttcctgacgcat ggcggcaaga aactgatggt ggcgtcttat 1020 gacgacagca tcgtcgccgcgatcgatctg gaaaaacgcg aactgatcaa gcaattgcca 1080 gcgggttgtg tgccgcacgtcggtggcggc gcggcggtcg tggttgatgg tcgtaccttg 1140 ggcttcggta ccaactttggcgattgcgac aagatggtcg tcagcgtttg ggatttggac 1200 aaaatggaag tcgtcaaacaagtaccggtt tcaggtggca ctgaatcgcc tgcggctcat 1260 gccaacgcac cttatgtcgcggttgacatc atcagcaaag acagacgtgc acgcaccatt 1320 cagttgatcg acaagaaaaccctggaagtt gccaaaactc tggatgtcgg cggccacgcc 1380 tacttcccgg aatatagcgccgacggcaaa ttcctctatg tcagtgccgg ctacaatggc 1440 gacgaagtcg tggtttacgattccaatacc ttgcaaaaag tggcgaccgt gccgatggaa 1500 agtcctgctg gtatcttctccagaggccgt gtcaaataca tgactcgcgg tctgtcacct 1560 gacgaaatgg agcaaggcaa a1581 60 527 PRT Methylomonas 16a NorS 60 Met Met Lys Thr Thr Thr Lys ArgArg Leu Asn Gln Ser Leu Leu Ala 1 5 10 15 Ser Ala Ile Ala Ala Leu LeuSer Ser Gly Ala Val Leu Ala Lys Ser 20 25 30 Asp Ser Pro His Asp Ile TyrMet Asp Asn Cys Ala Ser Cys His Gly 35 40 45 Ala Asp His Gly Gly Tyr LeuAla Pro Ala Leu Asn Ala Asp Thr Leu 50 55 60 Lys Gly Arg Ser Pro Thr AlaLeu Arg Thr Ile Val Met ala Gly Ser 65 70 75 80 Phe Asp Thr Leu Met ProPro Phe Tyr Gly Lys Leu Ser Asp Asp Glu 85 90 95 Ile Arg Gly Val Ile LysHis Leu Gln Glu Thr Pro Lys Gln Pro Asn 100 105 110 Pro Ala Trp Thr IleAsp Asp Met Lys Lys Ser Leu Lys Val Tyr Val 115 120 125 Lys Asp Glu SerThr Leu Pro Gly Lys Pro Thr Phe Gln Ile Asp Asn 130 135 140 Ile Asp AsnLeu Ile Gly Val Ala Ala Arg Gly Lys Tyr Gly Arg Gly 145 150 155 160 GluGly Ser Lys Ala Ile Phe Ile Asn Ser Thr Asn His Gln Lys Val 165 170 175Gly Glu Val Ala Thr Gly Thr Ala Ala His Ile Ile Asp Phe Asn Pro 180 185190 Ala Asn Pro Arg Trp Ala Tyr Val Lys Thr Asp Thr Ala Glu Ile Phe 195200 205 Lys Val Asp Leu Tyr Ser Met Gln Ala Val Arg Ser Ile Lys Thr Gly210 215 220 Tyr Asn Gly Pro Gly Met Gly Val Ser Arg Asp Gly Lys Tyr IleMet 225 230 235 240 Ala Gly Ser Phe Val Pro His Asn Ala Val Ile Leu AspAla Glu Thr 245 250 255 Leu Glu Pro Leu Lys Thr Phe Glu Leu Glu Gly IleAsp Pro Asp Gly 260 265 270 Lys His Val Ser Ser Asp Ser Gly Met Ile IleGly Thr Pro Tyr Ala 275 280 285 Asp Val Phe Ala Ile Ala Leu Glu Asn AlaGly Gln Val Trp Ile Val 290 295 300 Asp Tyr Asn Lys Glu Gly Phe Pro ValThr Lys Ile Glu Lys Val Gly 305 310 315 320 Arg His Leu His Asp Ala PheLeu Thr His Gly Gly Lys Lys Leu Met 325 330 335 Val Ala Ser Tyr Asp AspSer Ile Val Ala Ala Ile Asp Leu Glu Lys 340 345 350 Arg Glu Leu Ile LysGln Leu Pro Ala Gly Cys Val Pro His Val Gly 355 360 365 Gly Gly Ala AlaVal Val Val Asp Gly Arg Thr Leu Gly Phe Gly Thr 370 375 380 Asn Phe GlyAsp Cys Asp Lys Met Val Val Ser Val Trp Asp Leu Asp 385 390 395 400 LysMet Glu Val Val Lys Gln Val Pro Val Ser Gly Gly Thr Glu Ser 405 410 415Pro Ala Ala His Ala Asn Ala Pro Tyr Val Ala Val Asp Ile Ile Ser 420 425430 Lys Asp Arg Arg Ala Arg Thr Ile Gln Leu Ile Asp Lys Lys Thr Leu 435440 445 Glu Val Ala Lys Thr Leu Asp Val Gly Gly His Ala Tyr Phe Pro Glu450 455 460 Tyr Ser Ala Asp Gly Lys Phe Leu Tyr Val Ser Ala Gly Tyr AsnGly 465 470 475 480 Asp Glu Val Val Val Tyr Asp Ser Asn Thr Leu Gln LysVal Ala Thr 485 490 495 Val Pro Met Glu Ser Pro Ala Gly Ile Phe Ser ArgGly Arg Val Lys 500 505 510 Tyr Met Thr Arg Gly Leu Ser Pro Asp Glu MetGlu Gln Gly Lys 515 520 525 61 1860 DNA Methylomonas 16a DXS 61atgaaactga ccaccgacta tcccttgctt aaaaacatcc acacgccggc ggacatacgc 60gcgctgtcca aggaccagct ccagcaactg gctgacgagg tgcgcggcta tctgacccac 120acggtcagca tttccggcgg ccattttgcg gccggcctcg gcaccgtgga actgaccgtg 180gccttgcatt atgtgttcaa tacccccgtc gatcagttgg tctgggacgt gggccatcag 240gcctatccgc acaagattct gaccggtcgc aaggagcgca tgccgaccat tcgcaccctg 300ggcggggtgt cagcctttcc ggcgcgggac gagagcgaat acgatgcctt cggcgtcggc 360cattccagca cctcgatcag cgcggcactg ggcatggcca ttgcgtcgca gctgcgcggc 420gaagacaaga agatggtagc catcatcggc gacggttcca tcaccggcgg catggcctat 480gaggcgatga atcatgccgg cgatgtgaat gccaacctgc tggtgatctt gaacgacaac 540gatatgtcga tctcgccgcc ggtcggggcg atgaacaatt atctgaccaa ggtgttgtcg 600agcaagtttt attcgtcggt gcgggaagag agcaagaaag ctctggccaa gatgccgtcg 660gtgtgggaac tggcgcgcaa gaccgaggaa cacgtgaagg gcatgatcgt gcccggtacc 720ttgttcgagg aattgggctt caattatttc ggcccgatcg acggccatga tgtcgagatg 780ctggtgtcga ccctggaaaa tctgaaggat ttgaccgggc cggtattcct gcatgtggtg 840accaagaagg gcaaaggcta tgcgccagcc gagaaagacc cgttggccta ccatggcgtg 900ccggctttcg atccgaccaa ggatttcctg cccaaggcgg cgccgtcgcc gcatccgacc 960tataccgagg tgttcggccg ctggctgtgc gacatggcgg ctcaagacga gcgcttgctg 1020ggcatcacgc cggcgatgcg cgaaggctct ggtttggtgg aattctcaca gaaatttccg 1080aatcgctatt tcgatgtcgc catcgccgag cagcatgcgg tgaccttggc cgccggccag 1140gcctgccagg gcgccaagcc ggtggtggcg atttattcca ccttcctgca acgcggttac 1200gatcagttga tccacgacgt ggccttgcag aacttagata tgctctttgc actggatcgt 1260gccggcttgg tcggcccgga tggaccgacc catgctggcg cctttgatta cagctacatg 1320cgctgtattc cgaacatgct gatcatggct ccagccgacg agaacgagtg caggcagatg 1380ctgaccaccg gcttccaaca ccatggcccg gcttcggtgc gctatccgcg cggcaaaggg 1440cccggggcgg caatcgatcc gaccctgacc gcgctggaga tcggcaaggc cgaagtcaga 1500caccacggca gccgcatcgc cattctggcc tggggcagca tggtcacgcc tgccgtcgaa 1560gccggcaagc agctgggcgc gacggtggtg aacatgcgtt tcgtcaagcc gttcgatcaa 1620gccttggtgc tggaattggc caggacgcac gatgtgttcg tcaccgtcga ggaaaacgtc 1680atcgccggcg gcgctggcag tgcgatcaac accttcctgc aggcgcagaa ggtgctgatg 1740ccggtctgca acatcggcct gcccgaccgc ttcgtcgagc aaggtagtcg cgaggaattg 1800ctcagcctgg tcggcctcga cagcaagggc atcctcgcca ccatcgaaca gttttgcgct 186062 620 PRT Methylomonas 16a Amino acid sequences encoded by DXS 62 MetLys Leu Thr Thr Asp Tyr Pro Leu Leu Lys Asn Ile His Thr Pro 1 5 10 15Ala Asp Ile Arg Ala Leu Ser Lys Asp Gln Leu Gln Gln Leu Ala Asp 20 25 30Glu Val Arg Gly Tyr Leu Thr His Thr Val Ser Ile Ser Gly Gly His 35 40 45Phe Ala Ala Gly Leu Gly Thr Val Glu Leu Thr Val Ala Leu His Tyr 50 55 60Val Phe Asn Thr Pro Val Asp Gln Leu Val Trp Asp Val Gly His Gln 65 70 7580 Ala Tyr Pro His Lys Ile Leu Thr Gly Arg Lys Glu Arg Met Pro Thr 85 9095 Ile Arg Thr Leu Gly Gly Val Ser Ala Phe Pro Ala Arg Asp Glu Ser 100105 110 Glu Tyr Asp Ala Phe Gly Val Gly His Ser Ser Thr Ser Ile Ser Ala115 120 125 Ala Leu Gly Met ala Ile Ala Ser Gln Leu Arg Gly Glu Asp LysLys 130 135 140 Met Val Ala Ile Ile Gly Asp Gly Ser Ile Thr Gly Gly Metala Tyr 145 150 155 160 Glu Ala Met Asn His Ala Gly Asp Val Asn Ala AsnLeu Leu Val Ile 165 170 175 Leu Asn Asp Asn Asp Met Ser Ile Ser Pro ProVal Gly Ala Met Asn 180 185 190 Asn Tyr Leu Thr Lys Val Leu Ser Ser LysPhe Tyr Ser Ser Val Arg 195 200 205 Glu Glu Ser Lys Lys Ala Leu Ala LysMet Pro Ser Val Trp Glu Leu 210 215 220 Ala Arg Lys Thr Glu Glu His ValLys Gly Met Ile Val Pro Gly Thr 225 230 235 240 Leu Phe Glu Glu Leu GlyPhe Asn Tyr Phe Gly Pro Ile Asp Gly His 245 250 255 Asp Val Glu Met LeuVal Ser Thr Leu Glu Asn Leu Lys Asp Leu Thr 260 265 270 Gly Pro Val PheLeu His Val Val Thr Lys Lys Gly Lys Gly Tyr Ala 275 280 285 Pro Ala GluLys Asp Pro Leu Ala Tyr His Gly Val Pro Ala Phe Asp 290 295 300 Pro ThrLys Asp Phe Leu Pro Lys Ala Ala Pro Ser Pro His Pro Thr 305 310 315 320Tyr Thr Glu Val Phe Gly Arg Trp Leu Cys Asp Met ala Ala Gln Asp 325 330335 Glu Arg Leu Leu Gly Ile Thr Pro Ala Met Arg Glu Gly Ser Gly Leu 340345 350 Val Glu Phe Ser Gln Lys Phe Pro Asn Arg Tyr Phe Asp Val Ala Ile355 360 365 Ala Glu Gln His Ala Val Thr Leu Ala Ala Gly Gln Ala Cys GlnGly 370 375 380 Ala Lys Pro Val Val Ala Ile Tyr Ser Thr Phe Leu Gln ArgGly Tyr 385 390 395 400 Asp Gln Leu Ile His Asp Val Ala Leu Gln Asn LeuAsp Met Leu Phe 405 410 415 Ala Leu Asp Arg Ala Gly Leu Val Gly Pro AspGly Pro Thr His Ala 420 425 430 Gly Ala Phe Asp Tyr Ser Tyr Met Arg CysIle Pro Asn Met Leu Ile 435 440 445 Met ala Pro Ala Asp Glu Asn Glu CysArg Gln Met Leu Thr Thr Gly 450 455 460 Phe Gln His His Gly Pro Ala SerVal Arg Tyr Pro Arg Gly Lys Gly 465 470 475 480 Pro Gly Ala Ala Ile AspPro Thr Leu Thr Ala Leu Glu Ile Gly Lys 485 490 495 Ala Glu Val Arg HisHis Gly Ser Arg Ile Ala Ile Leu Ala Trp Gly 500 505 510 Ser Met Val ThrPro Ala Val Glu Ala Gly Lys Gln Leu Gly Ala Thr 515 520 525 Val Val AsnMet Arg Phe Val Lys Pro Phe Asp Gln Ala Leu Val Leu 530 535 540 Glu LeuAla Arg Thr His Asp Val Phe Val Thr Val Glu Glu Asn Val 545 550 555 560Ile Ala Gly Gly Ala Gly Ser Ala Ile Asn Thr Phe Leu Gln Ala Gln 565 570575 Lys Val Leu Met Pro Val Cys Asn Ile Gly Leu Pro Asp Arg Phe Val 580585 590 Glu Gln Gly Ser Arg Glu Glu Leu Leu Ser Leu Val Gly Leu Asp Ser595 600 605 Lys Gly Ile Leu Ala Thr Ile Glu Gln Phe Cys Ala 610 615 62063 1182 DNA Methylomonas 16a DXR 63 atgaaaggta tttgcatatt gggcgctaccggttcgatcg gtgtcagcac gctggatgtc 60 gttgccaggc atccggataa atatcaagtcgttgcgctga ccgccaacgg caatatcgac 120 gcattgtatg aacaatgcct ggcccaccatccggagtatg cggtggtggt catggaaagc 180 aaggtagcag agttcaaaca gcgcattgccgcttcgccgg tagcggatat caaggtcttg 240 tcgggtagcg aggccttgca acaggtggccacgctggaaa acgtcgatac ggtgatggcg 300 gctatcgtcg gcgcggccgg attgttgccgaccttggccg cggccaaggc cggcaaaacc 360 gtgctgttgg ccaacaagga agccttggtgatgtcgggac aaatcttcat gcaggccgtc 420 agcgattccg gcgctgtgtt gctgccgatagacagcgagc acaacgccat ctttcagtgc 480 atgccggcgg gttatacgcc aggccatacagccaaacagg cgcgccgcat tttattgacc 540 gcttccggtg gcccatttcg acggacgccgatagaaacgt tgtccagcgt cacgccggat 600 caggccgttg cccatcctaa atgggacatggggcgcaaga tttcggtcga ttccgccacc 660 atgatgaaca aaggtctcga actgatcgaagcctgcttgt tgttcaacat ggagcccgac 720 cagattgaag tcgtcattca tccgcagagcatcattcatt cgatggtgga ctatgtcgat 780 ggttcggttt tggcgcagat gggtaatcccgacatgcgca cgccgatagc gcacgcgatg 840 gcctggccgg aacgctttga ctctggtgtggcgccgctgg atattttcga agtagggcac 900 atggatttcg aaaaacccga cttgaaacggtttccttgtc tgagattggc ttatgaagcc 960 atcaagtctg gtggaattat gccaacggtattgaacgcag ccaatgaaat tgctgtcgaa 1020 gcgtttttaa atgaagaagt caaattcactgacatcgcgg tcatcatcga gcgcagcatg 1080 gcccagttta aaccggacga tgccggcagcctcgaattgg ttttgcaggc cgatcaagat 1140 gcgcgcgagg tggctagaga catcatcaagaccttggtag ct 1182 64 394 PRT Methylomonas 16a Amino acid sequencesencoded by DXR 64 Met Lys Gly Ile Cys Ile Leu Gly Ala Thr Gly Ser IleGly Val Ser 1 5 10 15 Thr Leu Asp Val Val Ala Arg His Pro Asp Lys TyrGln Val Val Ala 20 25 30 Leu Thr Ala Asn Gly Asn Ile Asp Ala Leu Tyr GluGln Cys Leu Ala 35 40 45 His His Pro Glu Tyr Ala Val Val Val Met Glu SerLys Val Ala Glu 50 55 60 Phe Lys Gln Arg Ile Ala Ala Ser Pro Val Ala AspIle Lys Val Leu 65 70 75 80 Ser Gly Ser Glu Ala Leu Gln Gln Val Ala ThrLeu Glu Asn Val Asp 85 90 95 Thr Val Met ala Ala Ile Val Gly Ala Ala GlyLeu Leu Pro Thr Leu 100 105 110 Ala Ala Ala Lys Ala Gly Lys Thr Val LeuLeu Ala Asn Lys Glu Ala 115 120 125 Leu Val Met Ser Gly Gln Ile Phe MetGln Ala Val Ser Asp Ser Gly 130 135 140 Ala Val Leu Leu Pro Ile Asp SerGlu His Asn Ala Ile Phe Gln Cys 145 150 155 160 Met Pro Ala Gly Tyr ThrPro Gly His Thr Ala Lys Gln Ala Arg Arg 165 170 175 Ile Leu Leu Thr AlaSer Gly Gly Pro Phe Arg Arg Thr Pro Ile Glu 180 185 190 Thr Leu Ser SerVal Thr Pro Asp Gln Ala Val Ala His Pro Lys Trp 195 200 205 Asp Met GlyArg Lys Ile Ser Val Asp Ser Ala Thr Met Met Asn Lys 210 215 220 Gly LeuGlu Leu Ile Glu Ala Cys Leu Leu Phe Asn Met Glu Pro Asp 225 230 235 240Gln Ile Glu Val Val Ile His Pro Gln Ser Ile Ile His Ser Met Val 245 250255 Asp Tyr Val Asp Gly Ser Val Leu Ala Gln Met Gly Asn Pro Asp Met 260265 270 Arg Thr Pro Ile Ala His Ala Met ala Trp Pro Glu Arg Phe Asp Ser275 280 285 Gly Val Ala Pro Leu Asp Ile Phe Glu Val Gly His Met Asp PheGlu 290 295 300 Lys Pro Asp Leu Lys Arg Phe Pro Cys Leu Arg Leu Ala TyrGlu Ala 305 310 315 320 Ile Lys Ser Gly Gly Ile Met Pro Thr Val Leu AsnAla Ala Asn Glu 325 330 335 Ile Ala Val Glu Ala Phe Leu Asn Glu Glu ValLys Phe Thr Asp Ile 340 345 350 Ala Val Ile Ile Glu Arg Ser Met ala GlnPhe Lys Pro Asp Asp Ala 355 360 365 Gly Ser Leu Glu Leu Val Leu Gln AlaAsp Gln Asp Ala Arg Glu Val 370 375 380 Ala Arg Asp Ile Ile Lys Thr LeuVal Ala 385 390 65 471 DNA Methylomonas 16a ISPF 65 atgatacgcgtaggcatggg ttacgacgtg caccgtttca acgacggcga ccacatcatt 60 ttgggcggcgtcaaaatccc ttatgaaaaa ggcctggaag cccattccga cggcgacgtg 120 gtgctgcacgcattggccga cgccatcttg ggagccgccg ctttgggcga catcggcaaa 180 catttcccggacaccgaccc caatttcaag ggcgccgaca gcagggtgct actgcgccac 240 gtgtacggcatcgtcaagga aaaaggctat aaactggtca acgccgacgt gaccatcatc 300 gctcaggcgccgaagatgct gccacacgtg cccggcatgc gcgccaacat tgccgccgat 360 ctggaaaccgatgtcgattt cattaatgta aaagccacga cgaccgagaa actgggcttt 420 gagggccgtaaggaaggcat cgccgtgcag gctgtggtgt tgatagaacg c 471 66 157 PRTMethylomonas 16a Amino acid sequences encoded by ISPF 66 Met Ile Arg ValGly Met Gly Tyr Asp Val His Arg Phe Asn Asp Gly 1 5 10 15 Asp His IleIle Leu Gly Gly Val Lys Ile Pro Tyr Glu Lys Gly Leu 20 25 30 Glu Ala HisSer Asp Gly Asp Val Val Leu His Ala Leu Ala Asp Ala 35 40 45 Ile Leu GlyAla Ala Ala Leu Gly Asp Ile Gly Lys His Phe Pro Asp 50 55 60 Thr Asp ProAsn Phe Lys Gly Ala Asp Ser Arg Val Leu Leu Arg His 65 70 75 80 Val TyrGly Ile Val Lys Glu Lys Gly Tyr Lys Leu Val Asn Ala Asp 85 90 95 Val ThrIle Ile Ala Gln Ala Pro Lys Met Leu Pro His Val Pro Gly 100 105 110 MetArg Ala Asn Ile Ala Ala Asp Leu Glu Thr Asp Val Asp Phe Ile 115 120 125Asn Val Lys Ala Thr Thr Thr Glu Lys Leu Gly Phe Glu Gly Arg Lys 130 135140 Glu Gly Ile Ala Val Gln Ala Val Val Leu Ile Glu Arg 145 150 155 67693 DNA Methylomonas 16a ISPD 67 atgaacccaa ccatccaatg ctgggccgtcgtgcccgcag ccggcgtcgg caaacgcatg 60 caagccgatc gccccaaaca atatttaccgcttgccggta aaacggtcat cgaacacaca 120 ctgactcgac tacttgagtc cgacgccttccaaaaagttg cggtggcgat ttccgtcgaa 180 gacccttatt ggcctgaact gtccatagccaaacaccccg acatcatcac cgcgcctggc 240 ggcaaggaac gcgccgactc ggtgctgtctgcactgaagg ctttagaaga tatagccagc 300 gaaaatgatt gggtgctggt acacgacgccgcccgcccct gcttgacggg cagcgacatc 360 caccttcaaa tcgatacctt aaaaaatgacccggtcggcg gcatcctggc cttgagttcg 420 cacgacacat tgaaacacgt ggatggtgacacgatcaccg caaccataga cagaaagcac 480 gtctggcgcg ccttgacgcc gcaaatgttcaaatacggca tgttgcgcga cgcgttgcaa 540 cgaaccgaag gcaatccggc cgtcaccgacgaagccagtg cgctggaact tttgggccat 600 aaacccaaaa tcgtggaagg ccgcccggacaacatcaaaa tcacccgccc ggaagatttg 660 gccctggcac aattttatat ggagcaacaagca 693 68 231 PRT Methylomonas 16a Amino acid sequences encoded by ISPD68 Met Asn Pro Thr Ile Gln Cys Trp Ala Val Val Pro Ala Ala Gly Val 1 510 15 Gly Lys Arg Met Gln Ala Asp Arg Pro Lys Gln Tyr Leu Pro Leu Ala 2025 30 Gly Lys Thr Val Ile Glu His Thr Leu Thr Arg Leu Leu Glu Ser Asp 3540 45 Ala Phe Gln Lys Val Ala Val Ala Ile Ser Val Glu Asp Pro Tyr Trp 5055 60 Pro Glu Leu Ser Ile Ala Lys His Pro Asp Ile Ile Thr Ala Pro Gly 6570 75 80 Gly Lys Glu Arg Ala Asp Ser Val Leu Ser Ala Leu Lys Ala Leu Glu85 90 95 Asp Ile Ala Ser Glu Asn Asp Trp Val Leu Val His Asp Ala Ala Arg100 105 110 Pro Cys Leu Thr Gly Ser Asp Ile His Leu Gln Ile Asp Thr LeuLys 115 120 125 Asn Asp Pro Val Gly Gly Ile Leu Ala Leu Ser Ser His AspThr Leu 130 135 140 Lys His Val Asp Gly Asp Thr Ile Thr Ala Thr Ile AspArg Lys His 145 150 155 160 Val Trp Arg Ala Leu Thr Pro Gln Met Phe LysTyr Gly Met Leu Arg 165 170 175 Asp Ala Leu Gln Arg Thr Glu Gly Asn ProAla Val Thr Asp Glu Ala 180 185 190 Ser Ala Leu Glu Leu Leu Gly His LysPro Lys Ile Val Glu Gly Arg 195 200 205 Pro Asp Asn Ile Lys Ile Thr ArgPro Glu Asp Leu Ala Leu Ala Gln 210 215 220 Phe Tyr Met Glu Gln Gln Ala225 230 69 1632 DNA Methylomonas 16a PYRG 69 atgacaaaat tcatctttatcaccggcggc gtggtgtcat ccttgggaaa agggatagcc 60 gcctcctccc tggcggcgattctggaagac cgcggcctca aagtcactat cacaaaactc 120 gatccctaca tcaacgtcgaccccggcacc atgagcccgt ttcaacacgg cgaggtgttc 180 gtgaccgaag acggtgccgaaaccgatttg gaccttggcc attacgaacg gtttttgaaa 240 accacgatga ccaagaaaaacaacttcacc accggtcagg tttacgagca ggtattacgc 300 aacgagcgca aaggtgattatcttggcgcg accgtgcaag tcattccaca tatcaccgac 360 gaaatcaaac gccgggtgtatgaaagcgcc gaagggaaag atgtggcatt gatcgaagtc 420 ggcggcacgg tgggcgacatcgaatcgtta ccgtttctgg aaaccatacg ccagatgggc 480 gtggaactgg gtcgtgaccgcgccttgttc attcatttga cgctggtgcc ttacatcaaa 540 tcggccggcg aactgaaaaccaagcccacc cagcattcgg tcaaagaact gcgcaccatc 600 gggattcagc cggacattttgatctgtcgt tcagaacaac cgatcccggc cagtgaacgc 660 cgcaagatcg cgctatttaccaatgtcgcc gaaaaggcgg tgatttccgc gatcgatgcc 720 gacaccattt accgcattccgctattgctg cgcgaacaag gcctggacga cctggtggtc 780 gatcagttgc gcctggacgtaccagcggcg gatttatcgg cctgggaaaa ggtcgtcgat 840 ggcctgactc atccgaccgacgaagtcagc attgcgatcg tcggtaaata tgtcgaccac 900 accgatgcct acaaatcgctgaatgaagcc ctgattcatg ccggcattca cacgcgccac 960 aaggtgcaaa tcagctacatcgactccgaa accatagaag ccgaaggcac cgccaaattg 1020 aaaaacgtcg atgcgatcctggtgccgggt ggtttcggcg aacgcggcgt ggaaggcaag 1080 atttctaccg tgcgttttgcccgcgagaac aaaatcccgt atttgggcat ttgcttgggc 1140 atgcaatcgg cggtaatcgaattcgcccgc aacgtggttg gcctggaagg cgcgcacagc 1200 accgaattcc tgccgaaatcgccacaccct gtgatcggct tgatcaccga atggatggac 1260 gaagccggcg aactggtcacacgcgacgaa gattccgatc tgggcggcac gatgcgtctg 1320 ggcgcgcaaa aatgccgcctgaaggctgat tccttggctt ttcagttgta tcaaaaagac 1380 gtcatcaccg agcgtcaccgccaccgctac gaattcaaca atcaatattt aaaacaactg 1440 gaagcggccg gcatgaaattttccggtaaa tcgctggacg gccgcctggt ggagatcatc 1500 gagctacccg aacacccctggttcctggcc tgccagttcc atcccgaatt cacctcgacg 1560 ccgcgtaacg gccacgccctattttcgggc ttcgtcgaag cggccgccaa acacaaaaca 1620 caaggcacag ca 1632 70544 PRT Methylomonas 16a Amino acid sequences encoded by ORF6 - PYRG 70Met Thr Lys Phe Ile Phe Ile Thr Gly Gly Val Val Ser Ser Leu Gly 1 5 1015 Lys Gly Ile Ala Ala Ser Ser Leu Ala Ala Ile Leu Glu Asp Arg Gly 20 2530 Leu Lys Val Thr Ile Thr Lys Leu Asp Pro Tyr Ile Asn Val Asp Pro 35 4045 Gly Thr Met Ser Pro Phe Gln His Gly Glu Val Phe Val Thr Glu Asp 50 5560 Gly Ala Glu Thr Asp Leu Asp Leu Gly His Tyr Glu Arg Phe Leu Lys 65 7075 80 Thr Thr Met Thr Lys Lys Asn Asn Phe Thr Thr Gly Gln Val Tyr Glu 8590 95 Gln Val Leu Arg Asn Glu Arg Lys Gly Asp Tyr Leu Gly Ala Thr Val100 105 110 Gln Val Ile Pro His Ile Thr Asp Glu Ile Lys Arg Arg Val TyrGlu 115 120 125 Ser Ala Glu Gly Lys Asp Val Ala Leu Ile Glu Val Gly GlyThr Val 130 135 140 Gly Asp Ile Glu Ser Leu Pro Phe Leu Glu Thr Ile ArgGln Met Gly 145 150 155 160 Val Glu Leu Gly Arg Asp Arg Ala Leu Phe IleHis Leu Thr Leu Val 165 170 175 Pro Tyr Ile Lys Ser Ala Gly Glu Leu LysThr Lys Pro Thr Gln His 180 185 190 Ser Val Lys Glu Leu Arg Thr Ile GlyIle Gln Pro Asp Ile Leu Ile 195 200 205 Cys Arg Ser Glu Gln Pro Ile ProAla Ser Glu Arg Arg Lys Ile Ala 210 215 220 Leu Phe Thr Asn Val Ala GluLys Ala Val Ile Ser Ala Ile Asp Ala 225 230 235 240 Asp Thr Ile Tyr ArgIle Pro Leu Leu Leu Arg Glu Gln Gly Leu Asp 245 250 255 Asp Leu Val ValAsp Gln Leu Arg Leu Asp Val Pro Ala Ala Asp Leu 260 265 270 Ser Ala TrpGlu Lys Val Val Asp Gly Leu Thr His Pro Thr Asp Glu 275 280 285 Val SerIle Ala Ile Val Gly Lys Tyr Val Asp His Thr Asp Ala Tyr 290 295 300 LysSer Leu Asn Glu Ala Leu Ile His Ala Gly Ile His Thr Arg His 305 310 315320 Lys Val Gln Ile Ser Tyr Ile Asp Ser Glu Thr Ile Glu Ala Glu Gly 325330 335 Thr Ala Lys Leu Lys Asn Val Asp Ala Ile Leu Val Pro Gly Gly Phe340 345 350 Gly Glu Arg Gly Val Glu Gly Lys Ile Ser Thr Val Arg Phe AlaArg 355 360 365 Glu Asn Lys Ile Pro Tyr Leu Gly Ile Cys Leu Gly Met GlnSer Ala 370 375 380 Val Ile Glu Phe Ala Arg Asn Val Val Gly Leu Glu GlyAla His Ser 385 390 395 400 Thr Glu Phe Leu Pro Lys Ser Pro His Pro ValIle Gly Leu Ile Thr 405 410 415 Glu Trp Met Asp Glu Ala Gly Glu Leu ValThr Arg Asp Glu Asp Ser 420 425 430 Asp Leu Gly Gly Thr Met Arg Leu GlyAla Gln Lys Cys Arg Leu Lys 435 440 445 Ala Asp Ser Leu Ala Phe Gln LeuTyr Gln Lys Asp Val Ile Thr Glu 450 455 460 Arg His Arg His Arg Tyr GluPhe Asn Asn Gln Tyr Leu Lys Gln Leu 465 470 475 480 Glu Ala Ala Gly MetLys Phe Ser Gly Lys Ser Leu Asp Gly Arg Leu 485 490 495 Val Glu Ile IleGlu Leu Pro Glu His Pro Trp Phe Leu Ala Cys Gln 500 505 510 Phe His ProGlu Phe Thr Ser Thr Pro Arg Asn Gly His Ala Leu Phe 515 520 525 Ser GlyPhe Val Glu Ala Ala Ala Lys His Lys Thr Gln Gly Thr Ala 530 535 540 71891 DNA Methylomonas 16a ORF7 ISPa 71 atgagtaaat tgaaagccta cctgaccgtctgccaagaac gcgtcgagcg cgcgctggac 60 gcccgtctgc ctgccgaaaa catactgccacaaaccttgc atcaggccat gcgctattcc 120 gtattgaacg gcggcaaacg cacccggcccttgttgactt atgcgaccgg tcaggctttg 180 ggcttgccgg aaaacgtgct ggatgcgccggcttgcgcgg tagaattcat ccatgtgtat 240 tcgctgattc acgacgatct gccggccatggacaacgatg atctgcgccg cggcaaaccg 300 acctgtcaca aggcttacga cgaggccaccgccattttgg ccggcgacgc actgcaggcg 360 ctggcctttg aagttctggc caacgaccccggcatcaccg tcgatgcccc ggctcgcctg 420 aaaatgatca cggctttgac ccgcgccagcggctctcaag gcatggtggg cggtcaagcc 480 atcgatctcg gctccgtcgg ccgcaaattgacgctgccgg aactcgaaaa catgcatatc 540 cacaagactg gcgccctgat ccgcgccagcgtcaatctgg cggcattatc caaacccgat 600 ctggatactt gcgtcgccaa gaaactggatcactatgcca aatgcatagg cttgtcgttc 660 caggtcaaag acgacattct cgacatcgaagccgacaccg cgacactcgg caagactcag 720 ggcaaggaca tcgataacga caaaccgacctaccctgcgc tattgggcat ggctggcgcc 780 aaacaaaaag cccaggaatt gcacgaacaagcagtcgaaa gcttaacggg atttggcagc 840 gaagccgacc tgctgcgcga actatcgctttacatcatcg agcgcacgca c 891 72 297 PRT Methylomonas 16a Amino acidsequences encoded by ORF7 - ISPa 72 Met Ser Lys Leu Lys Ala Tyr Leu ThrVal Cys Gln Glu Arg Val Glu 1 5 10 15 Arg Ala Leu Asp Ala Arg Leu ProAla Glu Asn Ile Leu Pro Gln Thr 20 25 30 Leu His Gln Ala Met Arg Tyr SerVal Leu Asn Gly Gly Lys Arg Thr 35 40 45 Arg Pro Leu Leu Thr Tyr Ala ThrGly Gln Ala Leu Gly Leu Pro Glu 50 55 60 Asn Val Leu Asp Ala Pro Ala CysAla Val Glu Phe Ile His Val Tyr 65 70 75 80 Ser Leu Ile His Asp Asp LeuPro Ala Met Asp Asn Asp Asp Leu Arg 85 90 95 Arg Gly Lys Pro Thr Cys HisLys Ala Tyr Asp Glu Ala Thr Ala Ile 100 105 110 Leu Ala Gly Asp Ala LeuGln Ala Leu Ala Phe Glu Val Leu Ala Asn 115 120 125 Asp Pro Gly Ile ThrVal Asp Ala Pro Ala Arg Leu Lys Met Ile Thr 130 135 140 Ala Leu Thr ArgAla Ser Gly Ser Gln Gly Met Val Gly Gly Gln Ala 145 150 155 160 Ile AspLeu Gly Ser Val Gly Arg Lys Leu Thr Leu Pro Glu Leu Glu 165 170 175 AsnMet His Ile His Lys Thr Gly Ala Leu Ile Arg Ala Ser Val Asn 180 185 190Leu Ala Ala Leu Ser Lys Pro Asp Leu Asp Thr Cys Val Ala Lys Lys 195 200205 Leu Asp His Tyr Ala Lys Cys Ile Gly Leu Ser Phe Gln Val Lys Asp 210215 220 Asp Ile Leu Asp Ile Glu Ala Asp Thr Ala Thr Leu Gly Lys Thr Gln225 230 235 240 Gly Lys Asp Ile Asp Asn Asp Lys Pro Thr Tyr Pro Ala LeuLeu Gly 245 250 255 Met ala Gly Ala Lys Gln Lys Ala Gln Glu Leu His GluGln Ala Val 260 265 270 Glu Ser Leu Thr Gly Phe Gly Ser Glu Ala Asp LeuLeu Arg Glu Leu 275 280 285 Ser Leu Tyr Ile Ile Glu Arg Thr His 290 29573 855 DNA Methylomonas 16a ISPE 73 atggattatg cggctgggtg gggcgaaagatggcctgctc cggcaaaatt gaacttaatg 60 ttgaggatta ccggtcgcag gccagatggctatcatctgt tgcaaacggt gtttcaaatg 120 ctcgatctat gcgattggtt gacgtttcatccggttgatg atggccgcgt gacgctgcga 180 aatccaatct ccggcgttcc agagcaggatgacttgactg ttcgggcggc taatttgttg 240 aagtctcata ccggctgtgt gcgcggagtttgtatcgata tcgagaaaaa tctgcctatg 300 ggtggtggtt tgggtggtgg aagttccgatgctgctacaa ccttggtagt tctaaatcgg 360 ctttggggct tgggcttgtc gaagcgtgagttgatggatt tgggcttgag gcttggtgcc 420 gatgtgcctg tgtttgtgtt tggttgttcggcctggggcg aaggtgtgag cgaggatttg 480 caggcaataa cgttgccgga acaatggtttgtcatcatta aaccggattg ccatgtgaat 540 actggagaaa ttttttctgc agaaaatttgacaaggaata gtgcagtcgt tacaatgagc 600 gactttcttg caggggataa tcggaatgattgttcggaag tggtttgcaa gttatatcga 660 ccggtgaaag atgcaatcga tgcgttgttatgctatgcgg aagcgagatt gacggggacc 720 ggtgcatgtg tgttcgctca gttttgtaacaaggaagatg ctgagagtgc gttagaagga 780 ttgaaagatc ggtggctggt gttcttggctaaaggcttga atcagtctgc gctctacaag 840 aaattagaac aggga 855 74 285 PRTMethylomonas 16a Amino acid sequences encoded by ISPE 74 Met Asp Tyr AlaAla Gly Trp Gly Glu Arg Trp Pro Ala Pro Ala Lys 1 5 10 15 Leu Asn LeuMet Leu Arg Ile Thr Gly Arg Arg Pro Asp Gly Tyr His 20 25 30 Leu Leu GlnThr Val Phe Gln Met Leu Asp Leu Cys Asp Trp Leu Thr 35 40 45 Phe His ProVal Asp Asp Gly Arg Val Thr Leu Arg Asn Pro Ile Ser 50 55 60 Gly Val ProGlu Gln Asp Asp Leu Thr Val Arg Ala Ala Asn Leu Leu 65 70 75 80 Lys SerHis Thr Gly Cys Val Arg Gly Val Cys Ile Asp Ile Glu Lys 85 90 95 Asn LeuPro Met Gly Gly Gly Leu Gly Gly Gly Ser Ser Asp Ala Ala 100 105 110 ThrThr Leu Val Val Leu Asn Arg Leu Trp Gly Leu Gly Leu Ser Lys 115 120 125Arg Glu Leu Met Asp Leu Gly Leu Arg Leu Gly Ala Asp Val Pro Val 130 135140 Phe Val Phe Gly Cys Ser Ala Trp Gly Glu Gly Val Ser Glu Asp Leu 145150 155 160 Gln Ala Ile Thr Leu Pro Glu Gln Trp Phe Val Ile Ile Lys ProAsp 165 170 175 Cys His Val Asn Thr Gly Glu Ile Phe Ser Ala Glu Asn LeuThr Arg 180 185 190 Asn Ser Ala Val Val Thr Met Ser Asp Phe Leu Ala GlyAsp Asn Arg 195 200 205 Asn Asp Cys Ser Glu Val Val Cys Lys Leu Tyr ArgPro Val Lys Asp 210 215 220 Ala Ile Asp Ala Leu Leu Cys Tyr Ala Glu AlaArg Leu Thr Gly Thr 225 230 235 240 Gly Ala Cys Val Phe Ala Gln Phe CysAsn Lys Glu Asp Ala Glu Ser 245 250 255 Ala Leu Glu Gly Leu Lys Asp ArgTrp Leu Val Phe Leu Ala Lys Gly 260 265 270 Leu Asn Gln Ser Ala Leu TyrLys Lys Leu Glu Gln Gly 275 280 285 75 1533 DNA Methylomonas 16a CRTN175 atggccaaca ccaaacacat catcatcgtc ggcgcgggtc ccggcggact ttgcgccggc 60atgttgctga gccagcgcgg cttcaaggta tcgattttcg acaaacatgc agaaatcggc 120ggccgcaacc gcccgatcaa catgaacggc tttaccttcg ataccggtcc gacattcttg 180ttgatgaaag gcgtgctgga cgaaatgttc gaactgtgcg agcgccgtag cgaggattat 240ctggaattcc tgccgctaag cccgatgtac cgcctgctgt acgacgaccg cgacatcttc 300gtctattccg accgcgagaa catgcgcgcc gaattgcaac gggtattcga cgaaggcacg 360gacggctacg aacagttcat ggaacaggaa cgcaaacgct tcaacgcgct gtatccctgc 420atcacccgcg attattccag cctgaaatcc tttttgtcgc tggacttgat caaggccctg 480ccgtggctgg cttttccgaa aagcgtgttc aataatctcg gccagtattt caaccaggaa 540aaaatgcgcc tggccttttg ctttcagtcc aagtatctgg gcatgtcgcc gtgggaatgc 600ccggcactgt ttacgatgct gccctatctg gagcacgaat acggcattta tcacgtcaaa 660ggcggcctga accgcatcgc ggcggcgatg gcgcaagtga tcgcggaaaa cggcggcgaa 720attcacttga acagcgaaat cgagtcgctg atcatcgaaa acggcgctgc caagggcgtc 780aaattacaac atggcgcgga gctgcgcggc gacgaagtca tcatcaacgc ggattttgcc 840cacgcgatga cgcatctggt caaaccgggc gtcttgaaaa aatacacccc ggaaaacctg 900aagcagcgcg agtattcctg ttcgaccttc atgctgtatc tgggtttgga caagatttac 960gatctgccgc accataccat cgtgtttgcc aaggattaca ccaccaatat ccgcaacatt 1020ttcgacaaca aaaccctgac ggacgatttt tcgttttacg tgcaaaacgc cagcgccagc 1080gacgacagcc tagcgccagc cggcaaatcg gcgctgtacg tgctggtgcc gatgcccaac 1140aacgacagcg gcctggactg gcaggcgcat tgccaaaacg tgcgcgaaca ggtgttggac 1200acgctgggcg cgcgactggg attgagcgac atcagagccc atatcgaatg cgaaaaaatc 1260atcacgccgc aaacctggga aacggacgaa cacgtttaca agggcgccac tttcagtttg 1320tcgcacaagt tcagccaaat gctgtactgg cggccgcaca accgtttcga ggaactggcc 1380aattgctatc tggtcggcgg cggcacgcat cccggtagcg gtttgccgac catctacgaa 1440tcggcgcgga tttcggccaa gctgatttcc cagaaacatc gggtgaggtt caaggacata 1500gcacacagcg cctggctgaa aaaagccaaa gcc 1533 76 511 PRT Methylomonas 16aAmino acid sequences encoded by CRTN1 76 Met ala Asn Thr Lys His Ile IleIle Val Gly Ala Gly Pro Gly Gly 1 5 10 15 Leu Cys Ala Gly Met Leu LeuSer Gln Arg Gly Phe Lys Val Ser Ile 20 25 30 Phe Asp Lys His Ala Glu IleGly Gly Arg Asn Arg Pro Ile Asn Met 35 40 45 Asn Gly Phe Thr Phe Asp ThrGly Pro Thr Phe Leu Leu Met Lys Gly 50 55 60 Val Leu Asp Glu Met Phe GluLeu Cys Glu Arg Arg Ser Glu Asp Tyr 65 70 75 80 Leu Glu Phe Leu Pro LeuSer Pro Met Tyr Arg Leu Leu Tyr Asp Asp 85 90 95 Arg Asp Ile Phe Val TyrSer Asp Arg Glu Asn Met Arg Ala Glu Leu 100 105 110 Gln Arg Val Phe AspGlu Gly Thr Asp Gly Tyr Glu Gln Phe Met Glu 115 120 125 Gln Glu Arg LysArg Phe Asn Ala Leu Tyr Pro Cys Ile Thr Arg Asp 130 135 140 Tyr Ser SerLeu Lys Ser Phe Leu Ser Leu Asp Leu Ile Lys Ala Leu 145 150 155 160 ProTrp Leu Ala Phe Pro Lys Ser Val Phe Asn Asn Leu Gly Gln Tyr 165 170 175Phe Asn Gln Glu Lys Met Arg Leu Ala Phe Cys Phe Gln Ser Lys Tyr 180 185190 Leu Gly Met Ser Pro Trp Glu Cys Pro Ala Leu Phe Thr Met Leu Pro 195200 205 Tyr Leu Glu His Glu Tyr Gly Ile Tyr His Val Lys Gly Gly Leu Asn210 215 220 Arg Ile Ala Ala Ala Met ala Gln Val Ile Ala Glu Asn Gly GlyGlu 225 230 235 240 Ile His Leu Asn Ser Glu Ile Glu Ser Leu Ile Ile GluAsn Gly Ala 245 250 255 Ala Lys Gly Val Lys Leu Gln His Gly Ala Glu LeuArg Gly Asp Glu 260 265 270 Val Ile Ile Asn Ala Asp Phe Ala His Ala MetThr His Leu Val Lys 275 280 285 Pro Gly Val Leu Lys Lys Tyr Thr Pro GluAsn Leu Lys Gln Arg Glu 290 295 300 Tyr Ser Cys Ser Thr Phe Met Leu TyrLeu Gly Leu Asp Lys Ile Tyr 305 310 315 320 Asp Leu Pro His His Thr IleVal Phe Ala Lys Asp Tyr Thr Thr Asn 325 330 335 Ile Arg Asn Ile Phe AspAsn Lys Thr Leu Thr Asp Asp Phe Ser Phe 340 345 350 Tyr Val Gln Asn AlaSer Ala Ser Asp Asp Ser Leu Ala Pro Ala Gly 355 360 365 Lys Ser Ala LeuTyr Val Leu Val Pro Met Pro Asn Asn Asp Ser Gly 370 375 380 Leu Asp TrpGln Ala His Cys Gln Asn Val Arg Glu Gln Val Leu Asp 385 390 395 400 ThrLeu Gly Ala Arg Leu Gly Leu Ser Asp Ile Arg Ala His Ile Glu 405 410 415Cys Glu Lys Ile Ile Thr Pro Gln Thr Trp Glu Thr Asp Glu His Val 420 425430 Tyr Lys Gly Ala Thr Phe Ser Leu Ser His Lys Phe Ser Gln Met Leu 435440 445 Tyr Trp Arg Pro His Asn Arg Phe Glu Glu Leu Ala Asn Cys Tyr Leu450 455 460 Val Gly Gly Gly Thr His Pro Gly Ser Gly Leu Pro Thr Ile TyrGlu 465 470 475 480 Ser Ala Arg Ile Ser Ala Lys Leu Ile Ser Gln Lys HisArg Val Arg 485 490 495 Phe Lys Asp Ile Ala His Ser Ala Trp Leu Lys LysAla Lys Ala 500 505 510 77 1491 DNA Methylomonas 16a CRTN2 77 atgaactcaaatgacaacca acgcgtgatc gtgatcggcg ccggcctcgg cggcctgtcc 60 gccgctatttcgctggccac ggccggcttt tccgtgcaac tcatcgaaaa aaacgacaag 120 gtcggcggcaagctcaacat catgaccaaa gacggcttta ccttcgatct ggggccgtcc 180 attttgacgatgccgcacat ctttgaggcc ttgttcacag gggccggcaa aaacatggcc 240 gattacgtgcaaatccagaa agtcgaaccg cactggcgca atttcttcga ggacggtagc 300 gtgatcgacttgtgcgaaga cgccgaaacc cagcgccgcg agctggataa acttggcccc 360 ggcacttacgcgcaattcca gcgctttctg gactattcga aaaacctctg cacggaaacc 420 gaagccggttacttcgccaa gggcctggac ggcttttggg atttactcaa gttttacggc 480 ccgctccgcagcctgctgag tttcgacgtc ttccgcagca tggaccaggg cgtgcgccgc 540 tttatttccgatcccaagtt ggtcgaaatc ctgaattact tcatcaaata cgtcggctcc 600 tcgccttacgatgcgcccgc cttgatgaac ctgctgcctt acattcaata tcattacggc 660 ctgtggtacgtgaaaggcgg catgtatggc atggcgcagg ccatggaaaa actggccgtg 720 gaattgggcgtcgagattcg tttagatgcc gaggtgtcgg aaatccaaaa acaggacggc 780 agagcctgcgccgtaaagtt ggcgaacggc gacgtgctgc cggccgacat cgtggtgtcg 840 aacatggaagtgattccggc gatggaaaaa ctgctgcgca gcccggccag cgaactgaaa 900 aaaatgcagcgcttcgagcc tagctgttcc ggcctggtgc tgcacttggg cgtggacagg 960 ctgtatccgcaactggcgca ccacaatttc ttttattccg atcatccgcg cgaacatttc 1020 gatgcggtattcaaaagcca tcgcctgtcg gacgatccga ccatttatct ggtcgcgccg 1080 tgcaagaccgaccccgccca ggcgccggcc ggctgcgaga tcatcaaaat cctgccccat 1140 atcccgcacctcgaccccga caaactgctg accgccgagg attattcagc cttgcgcgag 1200 cgggtgctggtcaaactcga acgcatgggc ctgacggatt tacgccaaca catcgtgacc 1260 gaagaatactggacgccgct ggatattcag gccaaatatt attcaaacca gggctcgatt 1320 tacggcgtggtcgccgaccg cttcaaaaac ctgggtttca aggcacctca acgcagcagc 1380 gaattatccaatctgtattt cgtcggcggc agcgtcaatc ccggcggcgg catgccgatg 1440 gtgacgctgtccgggcaatt ggtgagggac aagattgtgg cggatttgca a 1491 78 497 PRTMethylomonas 16a Amino acid sequences encoded by CRTN2 78 Met Asn SerAsn Asp Asn Gln Arg Val Ile Val Ile Gly Ala Gly Leu 1 5 10 15 Gly GlyLeu Ser Ala Ala Ile Ser Leu Ala Thr Ala Gly Phe Ser Val 20 25 30 Gln LeuIle Glu Lys Asn Asp Lys Val Gly Gly Lys Leu Asn Ile Met 35 40 45 Thr LysAsp Gly Phe Thr Phe Asp Leu Gly Pro Ser Ile Leu Thr Met 50 55 60 Pro HisIle Phe Glu Ala Leu Phe Thr Gly Ala Gly Lys Asn Met ala 65 70 75 80 AspTyr Val Gln Ile Gln Lys Val Glu Pro His Trp Arg Asn Phe Phe 85 90 95 GluAsp Gly Ser Val Ile Asp Leu Cys Glu Asp Ala Glu Thr Gln Arg 100 105 110Arg Glu Leu Asp Lys Leu Gly Pro Gly Thr Tyr Ala Gln Phe Gln Arg 115 120125 Phe Leu Asp Tyr Ser Lys Asn Leu Cys Thr Glu Thr Glu Ala Gly Tyr 130135 140 Phe Ala Lys Gly Leu Asp Gly Phe Trp Asp Leu Leu Lys Phe Tyr Gly145 150 155 160 Pro Leu Arg Ser Leu Leu Ser Phe Asp Val Phe Arg Ser MetAsp Gln 165 170 175 Gly Val Arg Arg Phe Ile Ser Asp Pro Lys Leu Val GluIle Leu Asn 180 185 190 Tyr Phe Ile Lys Tyr Val Gly Ser Ser Pro Tyr AspAla Pro Ala Leu 195 200 205 Met Asn Leu Leu Pro Tyr Ile Gln Tyr His TyrGly Leu Trp Tyr Val 210 215 220 Lys Gly Gly Met Tyr Gly Met ala Gln AlaMet Glu Lys Leu Ala Val 225 230 235 240 Glu Leu Gly Val Glu Ile Arg LeuAsp Ala Glu Val Ser Glu Ile Gln 245 250 255 Lys Gln Asp Gly Arg Ala CysAla Val Lys Leu Ala Asn Gly Asp Val 260 265 270 Leu Pro Ala Asp Ile ValVal Ser Asn Met Glu Val Ile Pro Ala Met 275 280 285 Glu Lys Leu Leu ArgSer Pro Ala Ser Glu Leu Lys Lys Met Gln Arg 290 295 300 Phe Glu Pro SerCys Ser Gly Leu Val Leu His Leu Gly Val Asp Arg 305 310 315 320 Leu TyrPro Gln Leu Ala His His Asn Phe Phe Tyr Ser Asp His Pro 325 330 335 ArgGlu His Phe Asp Ala Val Phe Lys Ser His Arg Leu Ser Asp Asp 340 345 350Pro Thr Ile Tyr Leu Val Ala Pro Cys Lys Thr Asp Pro Ala Gln Ala 355 360365 Pro Ala Gly Cys Glu Ile Ile Lys Ile Leu Pro His Ile Pro His Leu 370375 380 Asp Pro Asp Lys Leu Leu Thr Ala Glu Asp Tyr Ser Ala Leu Arg Glu385 390 395 400 Arg Val Leu Val Lys Leu Glu Arg Met Gly Leu Thr Asp LeuArg Gln 405 410 415 His Ile Val Thr Glu Glu Tyr Trp Thr Pro Leu Asp IleGln Ala Lys 420 425 430 Tyr Tyr Ser Asn Gln Gly Ser Ile Tyr Gly Val ValAla Asp Arg Phe 435 440 445 Lys Asn Leu Gly Phe Lys Ala Pro Gln Arg SerSer Glu Leu Ser Asn 450 455 460 Leu Tyr Phe Val Gly Gly Ser Val Asn ProGly Gly Gly Met Pro Met 465 470 475 480 Val Thr Leu Ser Gly Gln Leu ValArg Asp Lys Ile Val Ala Asp Leu 485 490 495 Gln 497 79 1455 DNAMETHYLOMONAS SP. 79 agacgttgct cctgtatcag cgttcttctc cggcttcgtttctatcatca tttacttctt 60 gtggcacttc ttcggccgtt ggttctcaaa aaccgacttcatcgccgacg atgcttctta 120 attgaagatt tgagaaaaat gacggctggc atgacttgcagtagattgcc agaagatctt 180 aattacaaat tctctagtaa tagaggagga aatatgaaaataattaaaga cagagttgca 240 aaactgtcct ttgtcgcact gctgatcact atgacagcagcgatgttcta cgctccaaca 300 gcatctgctc acggtgaaaa gtctcaagcg gctttcatgcgtatgcgtac cattcactgg 360 tttgacttga actggtcagc cgatgaagtt gctgtaaacgataccatgac aatttccggt 420 aaattccacg ttttcgctgg atggcctgaa actgttgataaaccagaagt ttctttcttg 480 aacatcggta ttcctggccc tgtatttatc cgtgcaggttcttggatcgg tggtcaattg 540 gttcctcgtt ctgtatcttt ggaactgggc gaaacttacgagtttaaagt actgttgaaa 600 gcacgtcgcc caggcgactg gcacgttcac accatgatgaacgttcaagg cggtggtcct 660 atcattggac caggtaaatg ggtaaccatt actggtaaaatgagcgattt cgttaaccca 720 gttactactc tgactggcca aacgatcgac ctcgaagactacgcgctgga caacgtttac 780 ttctggcacg cagtatggtt tgcaattggc tttgcttggttgattttctg gatcaaacgt 840 ccaatctttg ttccacgtca catcgctgtt agcactggtaaagcagactc tctgatctct 900 gctggcgaca aaaaagtagc catgatcttc ggtgttggtactctggttat cgttgctgct 960 tctatgggcg caaccaacga aaaataccca gtaaccactcctctgcaagc tggtttgttg 1020 cgtggtatga agccttatca aatgccagaa tctactgtttctgttaaagt tgatgacgct 1080 acctaccgtg taccaggtcg tgctatgcaa atgactctgaccatcaccaa caatggtgac 1140 tcagctgttc gtttgggtga gttcaacact gctggcgttcgtttcctgga ctcttctgtt 1200 catgaagatg aaaccggtta tccagatgac ttgttggctgaagatggttt gtctgttagc 1260 gacaacagcc cgattgctcc aggtgagact cgtactgttgaagttacagc ttctgacgct 1320 gcttgggaag tatatcgtct ggctgacttg atctatgacccagacagccg cttcgcaggt 1380 ctgatgttct tctgggacga aaacggcaac cgtcaaatgactatggttga cgctcctctg 1440 atcccaactt tcatc 1455 80 485 PRT METHYLOMONASSP. 80 Arg Arg Cys Ser Cys Ile Ser Val Leu Leu Arg Leu Arg Phe Tyr His 15 10 15 His Leu Leu Leu Val Ala Leu Leu Arg Pro Leu Val Leu Lys Asn Arg20 25 30 Leu His Arg Arg Arg Cys Phe Leu Ile Glu Asp Leu Arg Lys Met Thr35 40 45 Ala Gly Met Thr Cys Ser Arg Leu Pro Glu Asp Leu Asn Tyr Lys Phe50 55 60 Ser Ser Asn Arg Gly Gly Asn Met Lys Ile Ile Lys Asp Arg Val Ala65 70 75 80 Lys Leu Ser Phe Val Ala Leu Leu Ile Thr Met Thr Ala Ala MetPhe 85 90 95 Tyr Ala Pro Thr Ala Ser Ala His Gly Glu Lys Ser Gln Ala AlaPhe 100 105 110 Met Arg Met Arg Thr Ile His Trp Phe Asp Leu Asn Trp SerAla Asp 115 120 125 Glu Val Ala Val Asn Asp Thr Met Thr Ile Ser Gly LysPhe His Val 130 135 140 Phe Ala Gly Trp Pro Glu Thr Val Asp Lys Pro GluVal Ser Phe Leu 145 150 155 160 Asn Ile Gly Ile Pro Gly Pro Val Phe IleArg Ala Gly Ser Trp Ile 165 170 175 Gly Gly Gln Leu Val Pro Arg Ser ValSer Leu Glu Leu Gly Glu Thr 180 185 190 Tyr Glu Phe Lys Val Leu Leu LysAla Arg Arg Pro Gly Asp Trp His 195 200 205 Val His Thr Met Met Asn ValGln Gly Gly Gly Pro Ile Ile Gly Pro 210 215 220 Gly Lys Trp Val Thr IleThr Gly Lys Met Ser Asp Phe Val Asn Pro 225 230 235 240 Val Thr Thr LeuThr Gly Gln Thr Ile Asp Leu Glu Asp Tyr Ala Leu 245 250 255 Asp Asn ValTyr Phe Trp His Ala Val Trp Phe Ala Ile Gly Phe Ala 260 265 270 Trp LeuIle Phe Trp Ile Lys Arg Pro Ile Phe Val Pro Arg His Ile 275 280 285 AlaVal Ser Thr Gly Lys Ala Asp Ser Leu Ile Ser Ala Gly Asp Lys 290 295 300Lys Val Ala Met Ile Phe Gly Val Gly Thr Leu Val Ile Val Ala Ala 305 310315 320 Ser Met Gly Ala Thr Asn Glu Lys Tyr Pro Val Thr Thr Pro Leu Gln325 330 335 Ala Gly Leu Leu Arg Gly Met Lys Pro Tyr Gln Met Pro Glu SerThr 340 345 350 Val Ser Val Lys Val Asp Asp Ala Thr Tyr Arg Val Pro GlyArg Ala 355 360 365 Met Gln Met Thr Leu Thr Ile Thr Asn Asn Gly Asp SerAla Val Arg 370 375 380 Leu Gly Glu Phe Asn Thr Ala Gly Val Arg Phe LeuAsp Ser Ser Val 385 390 395 400 His Glu Asp Glu Thr Gly Tyr Pro Asp AspLeu Leu Ala Glu Asp Gly 405 410 415 Leu Ser Val Ser Asp Asn Ser Pro IleAla Pro Gly Glu Thr Arg Thr 420 425 430 Val Glu Val Thr Ala Ser Asp AlaAla Trp Glu Val Tyr Arg Leu Ala 435 440 445 Asp Leu Ile Tyr Asp Pro AspSer Arg Phe Ala Gly Leu Met Phe Phe 450 455 460 Trp Asp Glu Asn Gly AsnArg Gln Met Thr Met Val Asp Ala Pro Leu 465 470 475 480 Ile Pro Thr PheIle 485 81 1429 DNA METHYLOMONAS SP. 81 cggtatgctt aacacatgca agtcgaacgctgaagggtgc ttgcacctgg atgagtggcg 60 gacgggtgag taatgcatag gaatctgcctattagtgggg gataacgtgg ggaaactcac 120 gctaataccg catacgctct acggaggaaagccggggacc ttcgggcctg gcgctaatag 180 atgagcctat gtcggattag ctagttggtggggtaaaggc ctaccaaggc gacgatccgt 240 agctggtctg agaggatgat cagccacactgggactgaga cacggcccag actcctacgg 300 gaggcagcag tggggaatat tggacaatgggcgcaagcct gatccagcaa taccgcgtgt 360 gtgaagaagg cctgagggtt gtaaagcactttcaatggga aggaacacct atcggttaat 420 acccggtaga ctgacattac ccatacaagaagcaccggct aactccgtgc cagcagccgc 480 ggtaatacgg agggtgcaag cgttaatcggaattactggg cgtaaagcgt gcgtaggcgg 540 ttttttaagt cagatgtgaa agccctgggcttaacctggg aactgcattt gatactgggg 600 aactagagtt gagtagagga gagtggaatttcaggtgtag cggtgaaatg cgtagagatc 660 tgaaggaaca ccagtggcga aggcggctctctggactcaa actgacgctg aggtacgaaa 720 gcgtgggtag caaacaggat tagataccctggtagtccac gccgtaaacg atgtcaacta 780 accgttgggt tcttaaagaa cttagtggtggagctaacgt attaagttga ccgcctgggg 840 agtacggccg caaggctaaa actcaaatgaattgacgggg gcccgcacaa gcggtggagc 900 atgtggttta attcgatgca acgcgaagaaccttacctac ccttgacatc ctcggaactt 960 gtcagagatg acttggtgcc ttcgggaaccgagagacagg tgctgcatgg ctgtcgtcag 1020 ctcgtgtcgt gagatgttgg gttaagtcccgtaacgagcg caacccttat ccttagttgc 1080 cagcgcgtca tggcgggaac tctagggagactgccggtga taaaccggag gaaggtgggg 1140 acgacgtcaa gtcatcatgg cccttatgggtagggctaca cacgtgctac aatggtcggt 1200 acagagggtt gcgaactcgc gagagccagccaatcccaaa aagccgatcc tagtccggat 1260 tgcagtctgc aactcgactt gcatgaagtcggaatcgcta gtaatcgcgg atcagaatgc 1320 cgcggtgaat acgttcccgg gccttgtacacaccgcccgt cacaccatgg gagtgggttg 1380 caaaagaagt aggtagttta accttcgggagggcgcttac cactttgtg 1429

What is claimed is:
 1. A high growth methanotrophic bacterial strainwhich: (a) grows on a C1 carbon substrate selected from the groupconsisting of methane and methanol; and (b) comprises a functionalEmbden-Meyerhof carbon pathway, said pathway comprising a gene encodinga pyrophosphate dependent phosphofructokinase enzyme, the gene selectedfrom the group consisting of: (a) an isolated nucleic acid moleculeencoding the amino acid sequence as set forth in SEQ ID NO:6; (b) anisolated nucleic acid molecule that hybridizes with (a) under thefollowing hybridization conditions: 0.1×SSC, 0.1% SDS, 65° C. and washedwith 2×SSC, 0.1% SDS followed by 0.1×SSC, 0.1% SDS; (c) an isolatednucleic acid molecule comprising a first nucleotide sequence encoding apolypeptide of at least 437 amino acids that has at least 63% identitybased on the Smith-Waterman method of alignment when compared to apolypeptide having the sequence as set forth in SEQ ID NO:6; and (d) anisolated nucleic acid molecule that is complementary to (a), (b) or (c).2. A high growth methanotrophic bacterial strain according to claim 1wherein the strain optionally contains a functional Entner-Douderoffcarbon pathway.
 3. A bacterial strain according to claim 1 having atleast one gene encoding a fructose bisphosphate aldolase enzyme.
 4. Abacterial strain according to claim 3 wherein at least one gene encodesa fructose bisphosphate aldolase enzyme having the amino acid sequenceselected from the group consisting of SEQ ID NO:16 and SEQ ID NO:18. 5.A bacterial strain according to claim 2 having at least one geneencoding a keto-deoxy phosphogluconate aldolase.
 6. A bacterial strainaccording to claim 5 wherein at least one gene encodes a keto-deoxyphosphogluconate aldolase enzyme is selected from the group consistingof: (a) an isolated nucleic acid molecule encoding the amino acidsequence as set forth in SEQ ID NO:20; (b) an isolated nucleic acidmolecule that hybridizes with (a) under the following hybridizationconditions: 0.1×SSC, 0.1% SDS, 65° C. and washed with 2×SSC, 0.1% SDSfollowed by 0.1×SSC, 0.1% SDS; (c) an isolated nucleic acid moleculecomprising a first nucleotide sequence encoding a polypeptide of atleast 212 amino acids that has at least 59% identity based on theSmith-Waterman method of alignment when compared to a polypeptide havingthe sequence as set forth in SEQ ID NO:20; and (d) an isolated nucleicacid molecule that is complementary to (a), (b) or (c).
 7. A bacterialstrain according to any of claims 1 or 2 having a gene encoding apolypeptide involved in carbon flux wherein the polypeptide is selectedfrom the group consisting of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18and
 20. 8. A bacterial strain according to any of claims 1 or 2optionally comprising a denitrifying enzymatic pathway.
 9. The bacterialstrain of claim 8 wherein the enzymes of the denitrifying pathway arepolypeptides having the amino acid sequences selected from the groupconsisting of SEQ ID NO:40, 42, 44, 46, 48, 50, 52, 54, 56, 58 and 60.10. The bacterial strain of any of claims 1 or 2 having genes encodingexopolysaccharide synthesizing enzymes, the enzymes selected from thegroup consisting of SEQ ID NO:22, 24, 26, 28, 30, 32, 34, 36, and 38.11. The bacterial strain of any of claims 1 or 2 having genes encodingisoprenoid synthesizing enzymes, the enzymes selected from the groupconsisting of SEQ ID NO:62, 64, 66, 68, 70, 72, 74, 86, and
 78. 12. Thebacterial strain of claim 1 wherein the strain is a Methylomonas sp. 13.The bacterial strain of claim 12 having a 16s RNA profile as set forthin SEQ ID NO:81.
 14. The bacterial strain of claim 1 wherein, when theC1 carbon substrate is methanol, the strain produces glycogen comprisingat least about 50% dry weight of biomass. 15 The bacterial strain ofeither claim 1 or claim 14 wherein the methanol concentration in themedium is about 2.5% (vol/vol).
 16. The bacterial strain of any ofclaims 1 or 2 having a yield of greater than 1.0 grams of cell mass pergram of methane consumed.
 17. The bacterial strain of any of claims 1 or2 having a yield of greater than 0.5 grams of cell mass per gram ofmethane consumed.
 18. The bacterial strain of any of claims 1 or 2having a carbon conversion efficiency of greater than 40 g/molmethane/g/mol biomass.
 19. The bacterial strain of any of claims 1 or 2having a carbon conversion efficiency of greater than 65 g/molmethane/g/mol biomass.
 20. The bacterial strain of any of claims 1 or 2having a carbon conversion efficiency of greater than 70 g/molmethane/g/mol biomass.
 21. A high growth methanotrophic bacterial strainwhich grows on a C1 carbon substrate selected from the group consistingof methanol and methane, comprising the 16s RNA sequence as set forth inSEQ ID NO:81 and having at least one gene encoding a pyrophosphatedependent Phosphofructokinase enzyme.
 22. A high growth methanotrophicbacterial strain according to claim 20 optionally having at least onegene encoding a keto-deoxy phosphogluconate aldolase.
 23. A high growthmethanotrophic bacterial strain having the ATCC designation PTA 2402.24. A method for the production of single cell protein comprising: a)contacting the bacterial strains of any of the claims 1, 2, 3, 5, or 18with C1 carbon substrate, selected from the group consisting of methaneand methanol, in a suitable medium for a time sufficient to permit theexpression and accumulation of single cell protein; and b) optionallyrecovering the single cell protein.
 25. The method of claim 23 whereinthe C1 carbon substrate is contacted with the bacterial strain underanaerobic conditions.
 26. The method of claim 23 wherein the C1 carbonsubstrate is contacted with the bacterial strain under aerobicconditions.
 27. A method for the biotransformation of a nitrogencontaining compound selected from the group consisting of ammonia,nitrate, nitrite, and dinitrogen, comprising contacting the bacterialstrain of any of the claims 8 or 9 with a C1 carbon substrate selectedfrom the group consisting of methane or methanol, in the presence of thenitrogen containing compound, in a suitable medium for a time sufficientto permit the biotransformation of the nitrogen containing compound. 28.A method for the production of a feed product comprising protein,carbohydrates and pigment comprising the steps of: a) contacting thebacterial strain of any of claims 1, 2, 3, 5 or 18 with a C1 carbonsubstrate in a suitable medium for a time sufficient to permit theexpression and accumulation of the feed product; and b) optionallyrecovering the feed product.
 29. A method according to claim 28 whereinthe relative compositions of protein, carbohydrate and pigment arealtered through the up-regulation or down-regulation of any one of thegenes encoding the proteins selected from the group consisting of SEQ IDNO:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35,37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, and 69.30. A method of identifying the high growth methanotrophic bacterialstrain of claim 1 comprising: (a) growing a sample suspected ofcontaining a high growth methanotrophic bacterial strain on a suitablegrowth medium in the presence of methane as a sole carbon source; (b)identifying colonies that grow on the conditions of step (a); (c)analyzing the colonies identified in step (b) for the presence ofpyrophosphate dependent phosphofructokinase activity.
 31. A methodaccording to claim 30 wherein the colonies of step (b) are additionallyanalyzed for the presence of a gene selected from the group consistingof: (a) an isolated nucleic acid molecule encoding the amino acidsequence as set forth in SEQ ID NO:6; (b) an isolated nucleic acidmolecule that hybridizes with (a) under the following hybridizationconditions: 0.1×SSC, 0.1% SDS, 65° C. and washed with 2×SSC, 0.1% SDSfollowed by 0.1×SSC, 0.1% SDS; (c) an isolated nucleic acid moleculecomprising a first nucleotide sequence encoding a polypeptide of atleast 437 amino acids that has at least 63% identity based on theSmith-Waterman method of alignment when compared to a polypeptide havingthe sequence as set forth in SEQ ID NO:6; and (d) an isolated nucleicacid molecule that is complementary to (a), (b) or (c).